Mesorhizobium thiogangeticum sp. nov., a novel sulfur-oxidizing chemolithoautotroph from rhizosphere soil of an Indian tropical leguminous plant

Abstract

The bacterial strain SJT T , along with 15 other mesophilic, neutrophilic and facultatively sulfur-oxidizing chemolithotrophic isolates, was isolated by enrichment on reduced sulfur compounds as the sole energy and electron source from soils immediately adjacent to the roots of Clitoria ternatea, a slender leguminous herb of the Lower Gangetic plains of India. Strain SJT T was able to oxidize thiosulfate and elemental sulfur for chemolithoautotrophic growth. 16S rRNA and recA gene sequence-based phylogenetic analyses showed that the Gram-negative rod-shaped bacterium belonged to the genus Mesorhizobium and was most closely related to Mesorhizobium loti, Mesorhizobium plurifarium, Mesorhizobium amorphae and Mesorhizobium chacoense. Unequivocally low 16S rRNA (<97 %) and recA (¡88 %) gene sequence similarities to all existing species of the most closely related genera, a unique fatty acid profile, a distinct G+C content (59?6 mol%) and phenotypic characteristics all suggested that strain SJT T represents a novel species. DNA-DNA hybridization and SDS-PAGE analysis of whole-cell proteins also confirmed the taxonomic uniqueness of SJT T . It is therefore proposed that isolate SJT T (=LMG 22697 T =MTCC 7001 T ) be classified as the type strain of a novel species, Mesorhizobium thiogangeticum sp. nov.

Changes in bacterial community of anthracene bioremediation in municipal solid waste composting soil

“…Recently, some species of Mesorhizobium have been linked to sulfur oxidization (Ghosh and Roy, 2006), nitrile biotransformation (Feng et al, 2008), oxamyl degradation (Osborn et al, 2010), high arsenic resistance (Huang A.H. et al, 2010), chromium reducing (Wani et al, 2009), and acetonitrile biodegradation (Feng and Lee, 2009). However, little is known about the environmental significances of Terrimonas.…”

Mesorhizobium metallidurans sp. nov., a metal-resistant symbiont of Anthyllis vulneraria growing on metallicolous soil in Languedoc, France

“…Some of these species belong to the genus Mesorhizobium, which was first described by Jarvis et al (1997). At the time of writing, this genus comprises 12 recognized species, namely Mesorhizobium loti (Jarvis et al, 1982), M. huakuii (Chen et al, 1991), M. ciceri (Nour et al, 1994), M. mediterraneum (Nour et al, 1995), M. tianshanense (Chen et al, 1995), M. plurifarium (de Lajudie et al, 1998), M. amorphae (Wang et al, 1999), M. chacoense (Velazquez et al, 2001), M. septentrionale (Gao et al, 2004), M. temperatum (Gao et al, 2004), M. thiogangeticum (Ghosh & Roy, 2006) and M. albiziae (Wang et al, 2007).…”

Mesorhizobium ciceri biovar biserrulae, a novel biovar nodulating the pasture legume Biserrula pelecinus L.

“…At present, 11 species of Mesorhizobium have been proposed, ten of which, Mesorhizobium amorphae, M. chacoense, M. ciceri, M. huakuii, M. loti, M. mediterraneum, M. plurifarium, M. septentrionale, M. temperatum and M. tianshanense, have been shown to form nitrogen-fixing symbiotic associations with leguminous plants (Gao et al, 2004;Chen et al, 2005;Ghosh & Roy, 2006). All ten species share >97.7 % sequence similarity for the 16S rRNA gene and it is recommended that DNA-DNA hybridization values be included when describing novel species within this genus (Chen et al, 2005).…”