Mesoporous bioactive glass embedding propolis and cranberry antibiofilm compounds

Abstract

The aim of this study was to evaluate the chemical reactivity of 58S mesoporous bioactive glass (MBG) particles in as-synthesized condition and after embedding propolis and cranberry antibiofilm compounds at different concentrations. MBG 58S was synthesized by alkali sol-gel method with the addition of the triblock pluronic copolymer P123 as surfactant. Samples were characterized by physicochemical properties measurement, N adsorption/desorption analysis, and field emission gun scanning electron microscopy (FEGSEM) observations. MBG powders were immersed into 5 and 10 µg/mL propolis or cranberry solutions for 24 h. The chemical reactivity of the specimens was evaluated by FEGSEM, EDX, FTIR, Ca/P ratio, XRD, and sample weight gain analysis after being immersed in simulated body fluid (SBF) for 8, 24, and 72 h. MBG particles exhibited the expected chemical composition with a particle size distribution ranging from 1.44 to 955 µm, and a mean particle size of 154 µm. MBG particles exhibited a pore volume of 0.8 cc/g, pore radius of ∼2 nm, and surface area of 350.2 m /g, according to BJH and BET analyses. A hydroxyl-carbonate apatite (HCAp) layer was formed on all samples after SBF immersion for 72 h. Pure MBG showed the highest chemical reactivity after 72 h, with the resulting apatite layer exhibiting a Ca/P ratio of ∼1.6 in accordance to stoichiometric biological apatite. MBG embedding propolis and cranberry can be considered for future microbiological analysis since the presence of propolis or cranberry did not interfere with MBG's ability to develop a HCAp layer, which is an essential feature for bone regeneration applications. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1614-1625, 2018.

Effect of microwave and probe sonication processes on sol–gel‐derived bioactive glass and its structural and biocompatible investigations

“…In accordance with International Union of Pure and Applied Chemistry (IUPAC), pore sizes of the materials are classified into three types depending on the porosity. Mainly six types of adsorption/desorption isotherms are present, in which our results reveal the type IV characteristics with a hysteresis loop of mesoporous property for all the three bioactive glass . This mesoporous behavior of the bioglass enhances bioactivity and improves osteogenic and angiogenic properties …”

Synthesis and Characterization of a Highly Ordered Mesoporous Bio-glass

“…The BJH analysis showed the pore size distribution of the synthetic bioglass centered at 6.5 nm as seen in Figure 2. The specific surface area (SBET) and pore volume value (Vp) of the synthetic bio-glass were 395.6 (m 2 /g) and 0.52 (cm 2 /g), which are significantly higher than those achieved for conventional solgel bio-glasses of similar compositions [9,10,13].…”

“…This leads to forming the HA layer on the surface of the bioglasses [3,4]. In comparison with the previous studies [4,9,13], the synthetic bio-glass expressed the high bioactivity via a quick formation of a new-apatite layer after only 24 hours of the ''in vitro'' experiment. Figure 6 presents the ionic behaviors in the SBF solution as a function of immersion time, identified by the ICP-OES method.…”

“…Especially, the obtained bioglasses can have porous structures and high values of specific surface area that improve the bioactivity of the synthetic materials [7]. To enhance the porous structure and specific surface area of the bio-glasses synthesized by sol-gel method, Pluronic P123-a non-ionic block copolymer of poly(ethylene oxide)-poly (propylene oxide) -poly(ethylene oxide) [HO(CH2CH2O)20 (CH2CH(CH3)O)70 (CH2CH2O)20H] has often been used as a structure-creating template [8][9][10]. The selfassembly property of this organic compound leads to the highly ordered mesostructure of the synthetic bio-glasses.…”

“…The selfassembly property of this organic compound leads to the highly ordered mesostructure of the synthetic bio-glasses. Depending on the composition of the synthetic bio-glasses and the added amount of the structure-directing agent, the obtained bio-glasses have different properties of pore size, pore-volume, and specific surface area, which influenced the bioactivity ability of the final bio-glasses [9][10]. In this work, the bio-glass 58S with the composition of 58SiO2-33CaO-9P2O5 (wt.%) has been synthesized by the modified sol-gel method.…”