Mesenchymal stem cells can be easily isolated from bone marrow of patients with various haematological malignancies but the surface antigens expression may be changed after prolonged ex vivo culture

Yeh, Shih-Peng; Lo, Wen; Lee, C. C.; Lin, Chun-Cheng; Chiu, Chang‐Fang

doi:10.1038/sj.leu.2403795

Cited by 11 publications

(11 citation statements)

References 7 publications

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“…The morphology of CD45 þ MSCs is similar to that of CD45 À MSCs and the multilineage differentiation potential of CD45 þ MSCs is well preserved (1). However, the expression of CD45 was lost after prolonged ex vivo culture (14,15). MSCs can express CD45 under certain conditions.…”

Section: Discussionmentioning

confidence: 90%

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The effects of clinically used MRI contrast agents on the biological properties of human mesenchymal stem cells

Kim

Joo

et al. 2010

NMR in Biomedicine

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This study was undertaken to compare the labeling efficiencies of three iron-oxide based MRI contrast agents [Feridex, Resovist and monocrystalline iron oxide (MION)] and to evaluate their effects on the biological properties of human mesenchymal stem cells (hMSCs). The hMSCs were cultivated for 1 and 7 days after 24-h labeling with iron oxide nanoparticles (12.5 microg Fe/mL) in the presence of poly-L-lysine (0.75 microg/mL). The hMSCs were labeled more efficiently with use of Feridex, Resovist as compared to MION. No significant differences were observed in terms of viability and proliferation of labeled hMSCs. The level of Oct-4 mRNA increased in labeled hMSCs at day 1 and the cellular phenotype changed from CD45-/CD44+/CD29+ to CD45low/CD44+/CD29+ at day 7, which closely resembles the phenotype of fresh bone marrow-derived hMSCs. Our study has demonstrated that the Feridex or Resovist is the preferred labeling agent for hMSCs. There was a change in Oct-4 and CD45 expression after labeling.

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Section: Discussionmentioning

confidence: 90%

“…The hMSC line used in the present study had a CD45-/CD44þ/CD29þ phenotype. Magnetically labeled hMSCs had a CD45low/CD44þ/CD29þ phenotype, which closely resembles the phenotype of fresh bone marrow-derived MSCs (14,15).…”

Section: Biological Properties Of Hmscs Labeled With Iron Oxide Nanopmentioning

confidence: 94%

The effects of clinically used MRI contrast agents on the biological properties of human mesenchymal stem cells

Kim

Joo

et al. 2010

NMR in Biomedicine

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“…Also, Le Blanc et al showed IFN-c-treated MSCs expresses MHC-II [30]. Yeh et al have been illustrated that BM-MSCs isolated from patients with various hematological malignancies were CD45 + [31]. They have hypothesized that the abnormal marrow microenvironment of patients with hematological malignancies may have some influence on the surface antigens expression of MSCs.…”

Section: Discussionmentioning

confidence: 94%

The effect of pro-inflammatory cytokines on immunophenotype, differentiation capacity and immunomodulatory functions of human mesenchymal stem cells

et al. 2016

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“…In addition, a subpopulation of HPB-AML-I expressed CD45, even though most of HPB-AML-I cells were negative for CD45. Generally, CD45 is negative in MSCs, but CD45 expression has been detected in bone marrow MSCs from cases with multiple myeloma [30,31]. It is therefore not surprising that neoplastic MSC line, such as HPB-AML-I, shows the aberrant expression of this antigen.…”

Section: Discussionmentioning

confidence: 99%

The HPB-AML-I cell line possesses the properties of mesenchymal stem cells

Ardianto

Sugimoto

Kasagi

et al. 2010

J Exp Clin Cancer Res

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BackgroundIn spite of its establishment from the peripheral blood of a case with acute myeloid leukemia (AML)-M1, HPB-AML-I shows plastic adherence with spindle-like morphology. In addition, lipid droplets can be induced in HPB-AML-I cells by methylisobutylxanthine, hydrocortisone, and indomethacin. These findings suggest that HPB-AML-I is similar to mesenchymal stem cells (MSCs) or mesenchymal stromal cells rather than to hematopoietic cells.MethodsTo examine this possibility, we characterized HPB-AML-I by performing cytochemical, cytogenetic, and phenotypic analyses, induction of differentiation toward mesenchymal lineage cells, and mixed lymphocyte culture analysis.ResultsHPB-AML-I proved to be negative for myeloperoxidase, while surface antigen analysis disclosed that it was positive for MSC-related antigens, such as CD29, CD44, CD55, CD59, and CD73, but not for CD14, CD19, CD34, CD45, CD90, CD105, CD117, and HLA-DR. Karyotypic analysis showed the presence of complicated abnormalities, but no reciprocal translocations typically detected in AML cases. Following the induction of differentiation toward adipocytes, chondrocytes, and osteocytes, HPB-AML-I cells showed, in conjunction with extracellular matrix formation, lipid accumulation, proteoglycan synthesis, and alkaline phosphatase expression. Mixed lymphocyte culture demonstrated that CD3+ T-cell proliferation was suppressed in the presence of HPB-AML-I cells.ConclusionsWe conclude that HPB-AML-I cells appear to be unique neoplastic cells, which may be derived from MSCs, but are not hematopoietic progenitor cells.

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Mesenchymal stem cells can be easily isolated from bone marrow of patients with various haematological malignancies but the surface antigens expression may be changed after prolonged ex vivo culture

Cited by 11 publications

References 7 publications

The effects of clinically used MRI contrast agents on the biological properties of human mesenchymal stem cells

The effects of clinically used MRI contrast agents on the biological properties of human mesenchymal stem cells

The effect of pro-inflammatory cytokines on immunophenotype, differentiation capacity and immunomodulatory functions of human mesenchymal stem cells

The HPB-AML-I cell line possesses the properties of mesenchymal stem cells

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