Mesenchymal stem cell therapy of hepatocellular carcinoma in rats: Detection of cell homing and tumor mass by magnetic resonance imaging using iron oxide nanoparticles

Faidah, Mamdooh; Noorwali, Abdulwahab; Atta, Hazem; Ahmed, Naushad; Habıb, Hamid; Damiati, Laila A.; Filimban, Najlaa; Al-Qriqri, Mihal; Mahfouz, Soheir; Khabaz, Mohamad Nidal

doi:10.17219/acem/67563

Cited by 13 publications

(9 citation statements)

References 19 publications

(23 reference statements)

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“…In vivo tracking of stem cells using SPIONs has already been extensively studied in several animal models. For example, labeled MSCs were tracked in liver having hepatocellular carcinoma using MRI [42]. In another study, after transplantation of SPION-labeled MSCs in rats via the portal veins, they were visualized by MRI showing the improvement in the hepatic pathology and diminishing the cirrhosis [43].…”

Section: Discussionmentioning

confidence: 99%

MRI-Tracking of Dental Pulp Stem Cells In Vitro and In Vivo Using Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles

Zare

Mehrabani

Jalli

et al. 2019

JCM

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The aim of this study was to track dental pulp stem cells (DPSCs) labeled with dextran-coated superparamagnetic iron oxide nanoparticles (SPIONs) using magnetic resonance imaging (MRI). Dental pulp was isolated from male Sprague Dawley rats and cultured in Dulbecco’s modified Eagle’s medium F12 (DMEM-F12) and 10% fetal bovine serum. Effects of SPIONs on morphology, viability, apoptosis, stemness, and osteogenic and adipogenic differentiation of DPSCs were assessed. Prussian blue staining and MRI were conducted to determine in vitro efficiency of SPIONs uptake by the cells. Both non-labeled and labeled DPSCs were adherent to culture plates and showed spindle-shape morphologies, respectively. They were positive for osteogenic and adipogenic induction and expression of cluster of differentiation (CD) 73 and CD90 biomarkers, but negative for expression of CD34 and CD45 biomarkers. The SPIONs were non-toxic and did not induce apoptosis in doses less than 25 mg/mL. Internalization of the SPIONs within the DPSCs was confirmed by Prussian blue staining and MRI. Our findings revealed that the MRI-based method could successfully monitor DPSCs labeled with dextran-coated SPIONs without any significant effect on osteogenic and adipogenic differentiation, viability, and stemness of DPSCs. We provided the in vitro evidence supporting the feasibility of an MRI-based method to monitor DPSCs labeled with SPIONs without any significant reduction in viability, proliferation, and differentiation properties of labeled cells, showing that internalization of SPIONs within DPSCs were not toxic at doses less than 25 mg/mL. In general, the SPION labeling does not seem to impair cell survival or differentiation. SPIONs are biocompatible, easily available, and cost effective, opening a new avenue in stem cell labeling in regenerative medicine.

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Section: Discussionmentioning

confidence: 99%

MRI-Tracking of Dental Pulp Stem Cells In Vitro and In Vivo Using Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles

Zare

Mehrabani

Jalli

et al. 2019

JCM

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“…The procedure was performed in the Stem Cell Unit according to a previously published method [17]. MSCs were extracted and suspended in 25 cm 2 cell culture flasks (Corning, Corning, NY, USA) in DMEM media containing 10% FBS and supplemented with antibiotics (100 U/mL penicillin/streptomycin (Gibco, Life Technology, Carlsbad, CA, USA)) [15].…”

Section: Methodsmentioning

confidence: 99%

“…These cells were confirmed to be MSCs by flow cytometry (FACSAria III cell sorter model no. 6482-35, BD Bioscience, Erembodegem, Belgium) for detection of CD29 + /CD45 − cells [17]. For the gene expression studies, stem cells were seeded at a density of 3 × 10 5 cells/well in a 6-well cell culture plate.…”

Section: Methodsmentioning

confidence: 99%

Induction of Osteogenic Differentiation of Mesenchymal Stem Cells by Bioceramic Root Repair Material

et al. 2019

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This study aimed to evaluate the osteogenic activity of Endosequence Root Repair Material (ERRM) putty using rat mesenchymal stem cells (MSCs). The extract of set ERRM and ProRoot-mineral trioxide aggregate (MTA) (control) was cocultured with rat MSCs and incubated for one, three, and seven days. The cell viability and proliferation were assessed. A quantitative real-time polymerase chain reaction for bone morphogenetic protein-2 (BMP-2), alkaline phosphatase, bone sialoprotein, and osteocalcin gene expression was performed. Both materials enhanced cell viability and proliferation, which increased over time. On day seven, the cells treated with either material exhibited significantly greater cell viability compared with control untreated cells. MSCs treated with either material showed deeper alkaline phosphatase staining after three days compared to control untreated cells. Treated MSCs also exhibited upregulation of the gene expression of bone morphogenetic protein-2, alkaline phosphatase, bone sialoprotein, and osteocalcin. Both ERRM and ProRoot-MTA enhance the osteogenic differentiation of MSCs.

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“…Additionally, similar results by Lai and co-authors have shown that MSCs that overexpress human hepatocyte growth factor (HGF) promote liver recovery in a rat liver fibrosis model [120]. Further, labeling of MSC with NP led to an accumulation of the cells in MRI-based imaging [119,120], which seems to confirm the idea that magnetic NPs in liver fibrosis can be used as a diagnostic agent for MRI.…”

Section: Theoretical Background Of Magnetismmentioning

confidence: 52%

“…Labeling of MCS with MNPs can be used as a supplementary diagnostic approach. Recently, Faidah at al., proved that application of MNPs have not changed the viability and proliferative capabilities of MSCs in a rat cirrhosis model based on a carbon tetrachloride (CCl 4 ) model for toxic liver injury [119]. Additionally, similar results by Lai and co-authors have shown that MSCs that overexpress human hepatocyte growth factor (HGF) promote liver recovery in a rat liver fibrosis model [120].…”

Section: Theoretical Background Of Magnetismmentioning

confidence: 99%

Magnetic-Assisted Treatment of Liver Fibrosis

Levada

Omelyanchik

Rodionova

et al. 2019

Cells

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Chronic liver injury can be induced by viruses, toxins, cellular activation, and metabolic dysregulation and can lead to liver fibrosis. Hepatic fibrosis still remains a major burden on the global health systems. Nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH) are considered the main cause of liver fibrosis. Hepatic stellate cells are key targets in antifibrotic treatment, but selective engagement of these cells is an unresolved issue. Current strategies for antifibrotic drugs, which are at the critical stage 3 clinical trials, target metabolic regulation, immune cell activation, and cell death. Here, we report on the critical factors for liver fibrosis, and on prospective novel drugs, which might soon enter the market. Apart from the current clinical trials, novel perspectives for anti-fibrotic treatment may arise from magnetic particles and controlled magnetic forces in various different fields. Magnetic-assisted techniques can, for instance, enable cell engineering and cell therapy to fight cancer, might enable to control the shape or orientation of single cells or tissues mechanically. Furthermore, magnetic forces may improve localized drug delivery mediated by magnetism-induced conformational changes, and they may also enhance non-invasive imaging applications.

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Mesenchymal stem cell therapy of hepatocellular carcinoma in rats: Detection of cell homing and tumor mass by magnetic resonance imaging using iron oxide nanoparticles

Abstract: Background. Bone marrow-derived mesenchymal stem cells (MSCs) are reported to improve hepatic fibrosis, and may impact the signaling mechanisms leading to the induction of hepatocellular carcinoma (HCC) in animal models of liver cirrhosis.

Cited by 13 publications

References 19 publications

MRI-Tracking of Dental Pulp Stem Cells In Vitro and In Vivo Using Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles

MRI-Tracking of Dental Pulp Stem Cells In Vitro and In Vivo Using Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles

Induction of Osteogenic Differentiation of Mesenchymal Stem Cells by Bioceramic Root Repair Material

Magnetic-Assisted Treatment of Liver Fibrosis

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