Mesenchymal Stem Cell Isolation from Pulp Tissue and Co-Culture with Cancer Cells to Study Their Interactions

Doğan, Ayşegül; Demirci, Selami; Apdik, Hüseyin; Apdik, Ezgi Avşar; Şahın, Fikrettin

doi:10.3791/58825

Cited by 10 publications

(8 citation statements)

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“…Interestingly, another study found fresh Panax ginseng leaves, in association with spherical gold nanoparticles, to have suppressive effects on adipogenesis, simultaneously downregulating PPARγ/CEBPα signaling in 3T3-L1 mature adipocytes [55]. Boron was also found to inhibit adipogenesis in progenitor cells, by regulating; the β-catenin and AKT in Wnt/β-catenin pathways, the expression of adipogenesis related proteins, including PPARγ and cEBPα and regulation of cell cycle genes [56]. Wei et al demonstrated that the gradually decreasing age-dependent expression of the Hedgehog interacting protein, responsible for the interaction with hedgehog family and the modulation of hedgehog signaling, is involved in the down-regulation of the expression of Cyclin B, Cyclin D and Cyclin E, suppressing preadipocyte proliferation and increasing the expression of Glut4 and PPARγ [57].…”

Section: External Stimuli Regulating Proliferation Of Adipocytesmentioning

confidence: 99%

The Proliferation and Differentiation of Adipose-Derived Stem Cells in Neovascularization and Angiogenesis

Hutchings

Janowicz

Moncrieff

et al. 2020

IJMS

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Neovascularization and angiogenesis are vital processes in the repair of damaged tissue, creating new blood vessel networks and increasing oxygen and nutrient supply for regeneration. The importance of Adipose-derived Mesenchymal Stem Cells (ASCs) contained in the adipose tissue surrounding blood vessel networks to these processes remains unknown and the exact mechanisms responsible for directing adipogenic cell fate remain to be discovered. As adipose tissue contains a heterogenous population of partially differentiated cells of adipocyte lineage; tissue repair, angiogenesis and neovascularization may be closely linked to the function of ASCs in a complex relationship. This review aims to investigate the link between ASCs and angiogenesis/neovascularization, with references to current studies. The molecular mechanisms of these processes, as well as ASC differentiation and proliferation are described in detail. ASCs may differentiate into endothelial cells during neovascularization; however, recent clinical trials have suggested that ASCs may also stimulate angiogenesis and neovascularization indirectly through the release of paracrine factors.

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Section: External Stimuli Regulating Proliferation Of Adipocytesmentioning

confidence: 99%

The Proliferation and Differentiation of Adipose-Derived Stem Cells in Neovascularization and Angiogenesis

Hutchings

Janowicz

Moncrieff

et al. 2020

IJMS

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“…De Souza and colleagues have shown that mesenchymal stem cells (MSC) increase viability and hormone production capacity of islets cells [19] and Jiang and colleagues reported a regulatory effect by endothelial cells on microglial cells [20]. A stimulatory effect by co-culturing on cell migration of cancer cells and on ovarian differentiation was reported by Dogan et al [21] and Mackay et al [22]. Our data are in line with results of other authors that co-cultivation of different cell types can have a signi cant effect on hormone production.…”

Section: Discussionmentioning

confidence: 99%

Co-cultivation of human granulosa cells with ovarian cancer cells leads to a significant increase in progesterone production

Pietrowski

Grgic

Haslinger

et al. 2023

Arch Gynecol Obstet

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Purpose In humans, granulosa cells (GCs) are part of the follicle and nourish the growing oocyte. GCs produce estrogen and, after ovulation, progesterone. They are embedded in a multicellular tissue structure of the ovary, which consists of a variety of different cell types that are essential for the physiological function of the ovary. However, the extent to which individual ovarian cell types contribute to overall functionality has not yet been fully elucidated. In this study, we aim to investigate the effects of co-culturing human granulosa cells with ovarian cancer cells on their progesterone and estrogen production in an in vitro model. Methods After seeding, the cells were stimulated with 200 µM forskolin in DMEM for 72 h and the medium of the different cell culture experiments was collected. Subsequently, progesterone and oestradiol concentrations were determined using an Elisa assay. Results Morphologically, it was striking that the cells self-organize and form spatially separated areas. Compared to culturing granulosa cells alone, co-culturing human granulosa cells together with the ovarian cancer cell line OvCar-3 resulted in a significant increase in progesterone production (20.3 ng/ml versus 50.2 ng/ml; p < 0.01). Conclusions Using a simple in vitro model, we highlight the importance of cellular crosstalk between different ovarian cells in a complex cellular network and that it strongly influences granulosa cell hormone production. This could have potential implications for the procedure of transplanting endocrine tissues after cryopreservation, as it highlights the importance of survival of all cells for the functionality of the transplanted tissue.

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“…In this experiment, we determined the effect of soluble mediators on Treg/Th17 cells polarization through conditioned medium application, which is the simplest method for cell interaction studies. The advantage of conditioned medium is that it can avoid the influence of target cells on conditioned cells, focus on the effect of conditioned cells on target cells, and allow observation of the activity of soluble factors ( 56 , 57 ). As one of the methods of indirect co-culture, conditioned medium is very effective to observe the interaction between cells in vitro .…”

Section: Discussionmentioning

confidence: 99%

IL-7-Treated Periodontal Ligament Cells Regulate Local Immune Homeostasis by Modulating Treg/Th17 Cell Polarization

Zhang

Huang

et al. 2022

Front. Med.

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Both interleukin (IL)-7 and human periodontal ligament cells (hPDLCs) have immunomodulatory properties. However, their combined effect on CD4+T cells has never been studied. In this study, we aimed to investigate the effect of conditioned medium of hPDLCs treated with rhIL-7 on the differentiation of CD4+T cells into regulatory T cells/T helper 17 cells (Treg/Th17 cells) and observe the effect of IL-7 on the immunomodulatory properties of PDLCs. After hPDLCs were treated with different concentrations of rhIL-7 for 24 h, the collected supernatants were used to incubate CD4+T cells for 3 days. A gamma-secretase inhibitor (DAPT) was used to suppress the activation of the Notch1 signaling pathway. Cell proliferation, apoptosis, and necrosis were determined using the cell counting kit-8 (CCK-8) and flow cytometry (FCM). The expressions of forkhead box P3 (Foxp3) in CD4+T cells and transforming growth factor (TGF-β) and IL-6 in the supernatants were determined by ELISA. Reverse transcription-quantitative PCR (RT-qPCR), and the Western blot (WB) determined the mRNA levels and protein expression of various target factors. FCM was used to detect the mean fluorescence intensity of PD-L1 in hPDLCs and to analyze the differentiation of Treg/Th17 cells. Our results showed that IL-7 promoted proliferation and inhibited apoptosis in hPDLCs, promoted the expression of TGF-β, PD-L1, Notch1, Jagged1, and Hes1, and inhibited the levels of hypoxia-inducible factor (HIF)-1α and TCF7, whereas the addition of DAPT effectively reversed these effects. Importantly, we found that the conditioned medium of hPDLCs treated with rhIL-7 promoted the polarization of CD4+T cells into Treg cells but had no significant effect on the differentiation of Th17 cells. Our study indicated that treatment of PDLCs with IL-7 can promote the polarization of CD4+T cells into Treg cells by modulating the expression of inflammatory factors and signaling molecules through activating the Notch1 signaling pathway, thus participating in the regulation of immune homeostasis in the periodontal microenvironment.

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Mesenchymal Stem Cell Isolation from Pulp Tissue and Co-Culture with Cancer Cells to Study Their Interactions

Cited by 10 publications

References 0 publications

The Proliferation and Differentiation of Adipose-Derived Stem Cells in Neovascularization and Angiogenesis

The Proliferation and Differentiation of Adipose-Derived Stem Cells in Neovascularization and Angiogenesis

Co-cultivation of human granulosa cells with ovarian cancer cells leads to a significant increase in progesterone production

IL-7-Treated Periodontal Ligament Cells Regulate Local Immune Homeostasis by Modulating Treg/Th17 Cell Polarization

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