Mesenchymal stem cell–conditioned medium reduces liver injury and enhances regeneration in reduced-size rat liver transplantation

Du, Zhifeng; Wei, Cuifeng; Cheng, Kun; Han, Baosan; Yan, Jiqi; Zhang, Mingjun; Peng, Chenghong; Liu, Yingbin

doi:10.1016/j.jss.2013.02.009

Cited by 84 publications

(73 citation statements)

References 37 publications

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“…In the MSC-CM group the authors observed a significantly lower release of liver injury biomarkers, a reduction in hepatocyte apoptosis, an increased proliferation of hepatocytes and sinusoidal endothelial cells, and reduced signs of inflammation in the graft as assessed by lower expression of several proinflammatory cytokines, reduced neutrophil infiltration and Kupffer cell activation and increased expression of vascular endothelial growth factor and matrix metallopeptidase (or metalloproteinase) 9. These obser vations were associated with increased survival in the MSC-CM group compared with the control group [18]. (Kuppfer cells were first observed by Karl Wilhelm von Kupffer in 1876 and are hepatic macrophages.…”

Section: Animal Studiesmentioning

confidence: 92%

“…For example, allogeneic human MSCs are able to restore alveolar fluid clearance in human lungs through the secretion of keratinocyte growth factor [17]. The importance of these soluble factors secreted by MSCs is also illustrated by the fact that MSC-conditioned medium (MSC CM) has been found effective in reducing inflammation and improving survival in an animal model of LT [18]. Further information on the MSC secretome is given in Chapters 18 and 19.…”

Section: Tissue and Organ Regenerationmentioning

confidence: 99%

See 1 more Smart Citation

The use of mesenchymal stromal cells in solid organ transplantation

Delens

Jouret

Detry

et al. 2016

The Biology and Therapeutic Application of Mesenchymal Cells

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Section: Animal Studiesmentioning

confidence: 92%

Section: Tissue and Organ Regenerationmentioning

confidence: 99%

The use of mesenchymal stromal cells in solid organ transplantation

Delens

Jouret

Detry

et al. 2016

The Biology and Therapeutic Application of Mesenchymal Cells

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“…(15) Some studies in rodents clearly shown a good results of MSC in promoting regeneration of damaged tissues without any significance engraftment (48,49), and these support the idea of MSC's paracrine activity in mediating tissue repair by secretes bioactive molecules such as interleukins (IL) and tumor growth factors (TGF) that promote regenerative microenvirontment in the injury vicinity. (15,16,50,51) To date, therapeutic response of CM has been tested in disease models for lung injury (15), chronic kidney disease (50) and liver injury (16,51), among others, and demonstrate that MSC-CM alone is sufficient to mediate lasting therapeutic effects.…”

Section: Msc As Medicinal Signaling Cellmentioning

confidence: 99%

Mesenchymal Stem Cells Manage Endogenous Tissue Regeneration

2016

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BACKGROUND: Current findings set a new understanding that every adult tissue has its own intrinsic progenitor or stem cell, give a potency for their innate turnover dynamics. This broke the old assumption that adult tissues cannot regenerate themselves. Localized tissue regeneration was regulatory oversight by a separate class of local cells originating as perivascular cells, suggested a profound influence on using specific cells for cell therapies as a health care delivery tool set.CONTENT: The mesenchymal stem cell (MSC) could be mobilized from the marrow or other depots or can be culture-expanded MSCs which are delivered to the damage site either by direct or systemic injection. MSCs act paracrine and autocrine by inducing a variety of cytokines and growth factors which suppress local immune system, inhibit fibrosis (scar formation) and apoptosis, enhanceangiogenesis, and stimulate mitosis and differentiation of tissue, intrinsic reparative or stem cells. These referred a trophic effects, different from the direct differentiation of MSCs into repair tissue. Thus, MSC suggested as a multidrug delivery vehicles in response of injury. In this regard, the trophic effects of MSCs may have profound clinical use.SUMMARY: Managing the body’s natural repair and regeneration capacities is the new frontier for modern medicine and the basis for the science of cell therapies. Study of MSCs become one avenue that being pursued to explore the endogenous tissue regeneration management, so that people have a great expectation to solve many severe diseases.KEYWORDS: mesenchymal stromal/stem cell, paracrine or autocrine activities, trophic mediator, inflammation, wound healing

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“…[9][10][11] However, the efficacy of such conditioned medium has been debated. Previous studies have argued that injection of the MSC supernatant could mediate only the early steps of the tissue repair process due to its massive, uncontrollable, and short-term effects after transplantation in vivo.…”

Section: Introductionmentioning

confidence: 99%

Freeze-Dried Rat Bone Marrow Mesenchymal Stem Cell Paracrine Factors: A Simplified Novel Material for Skin Wound Therapy

Peng

Xuan

Zou

et al. 2015

Tissue Engineering Part A

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The mesenchymal stem cell (MSC) supernatant is well known as a rich source of autologous cytokines and universally used for tissue regeneration in current clinical medicine. However, the limitation of conditioned medium used in open-wound repair compels the need to find a more sophisticated way to take advantage of the trophic factors of MSCs. We have now fabricated a three-dimensional membrane from freeze-dried bone marrow mesenchymal stem cells-conditioned medium (FBMSC-CM) using a simple freeze-dried protocol. Scanning electron microscopy images showed the microstructure of the FBMSC-CM membrane (FBMSC-CMM) resembling a mesh containing growth factors. ELISA was used to test the paracrine factors retained in the FBMSC-CMM, and the results indicated that FBMSC-CMM withheld over 80% of the paracrine factors. Live/dead assays were adopted to test the toxicity of the FBMSC-CMM on cultured rat dermal fibroblasts, and the results confirmed its biological safety with low toxicity. Moreover, the FBMSC-CMM could significantly accelerate wound healing and enhance the neovascularization as well as epithelialization through strengthening the trophic factors in the wound bed as determined by immunohistochemical staining. Thus, the ability to maintain paracrine factors and enhance the effectiveness of these growth factors in the wound as well as the simple procedure and economical materials required for production qualifies the FBMSC-CMM to be a candidate biomaterial for open-wound regeneration.

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Mesenchymal stem cell–conditioned medium reduces liver injury and enhances regeneration in reduced-size rat liver transplantation

Cited by 84 publications

References 37 publications

The use of mesenchymal stromal cells in solid organ transplantation

The use of mesenchymal stromal cells in solid organ transplantation

Mesenchymal Stem Cells Manage Endogenous Tissue Regeneration

Freeze-Dried Rat Bone Marrow Mesenchymal Stem Cell Paracrine Factors: A Simplified Novel Material for Skin Wound Therapy

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