Mesenchymal and trophoblast immunophenotype of multipotent stromal cells from human placenta

Shablii, Volodymyr; Кучма, М. Д.; Кyryk, V.; Svitina, Hanna; Shablii, Yu.; Лукаш, Л. Л.; Лобинцева, Г. С.

doi:10.7124/bc.000889

Cited by 3 publications

(4 citation statements)

References 10 publications

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“…In the present study, rat and human PDMCs were characterized and it was demonstrated that they share similar surface immunophenotypes, with the exception of a lower expression of CD44 in rPDMCs. Furthermore, it has previously been demonstrated that hPDMCs express CD73 and CD105, but not CD34 or CD14 ( 40 ) and differentiated into osteogenic, adipogenic and chondrogenic lineages ( 41 ), thus fulfilling the minimal criteria for multipotent MSCs ( 42 ). Despite the absence of a classic mesenchymal surface immunophenotype ( 42 ), rPDMCs possessed the ability to differentiate into mesodermal cell lineages (adipogenic and osteogenic).…”

Section: Discussionmentioning

confidence: 99%

Placenta-derived multipotent cells have no effect on the size and number of DMH-induced colon tumors in rats

Svitina

Кyryk

Skrypkina

et al. 2017

Experimental and Therapeutic Medicine

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Transplantation of placenta-derived multipotent cells (PDMCs) is a promising approach for cell therapy to treat inflammation-associated colon diseases. However, the effect of PDMCs on colon cancer cells remains unknown. The aim of the present study was to characterize PDMCs obtained from human (hPDMCs) and rat (rPDMCs) placentas and to evaluate their impact on colon cancer progression in rats. PDMCs were obtained from human and rat placentas by tissue explant culturing. Stemness- and trophoblast-related gene expression was studied using reverse transcription-polymerase chain reaction (RT-PCR), and surface markers and intracellular proteins were detected using flow cytometry and immunofluorescence, respectively. Experimental colon carcinogenesis was induced in male albino Wistar rats by injecting 20 mg/kg dimethylhydrazine (DMH) once a week for 20 consecutive weeks. The administration of rPDMCs and hPDMC was performed at week 22 after the initial DMH-injection. All animals were sacrificed through carbon dioxide asphyxiation at week 5 after cell transplantation. The number and size of each tumor lesion was calculated. The type of tumor was determined by standard histological methods. Cell engraftment was determined by PCR and immunofluorescence. Results demonstrated that rPDMCs possessed the immunophenotype and differentiation potential inherent in MSCs; however, hPDMCs exhibited a lower expression of cluster of differentiation 44 and did not express trophoblast-associated genes. The data of the present study indicated that PDMCs may engraft in different tissues but do not significantly affect DMH-induced tumor growth during short-term observations.

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Section: Discussionmentioning

confidence: 99%

Placenta-derived multipotent cells have no effect on the size and number of DMH-induced colon tumors in rats

Svitina

Кyryk

Skrypkina

et al. 2017

Experimental and Therapeutic Medicine

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“…Various sources of obtaining human MSCs are indicated in the literature [61][62][63][64][65]. MSCs obtained from adipose tissue are the most studied [11], although stem cells of other origins are also used.…”

Section: Table 1 Examples Of Experimental Wound Healing Activity Stud...mentioning

confidence: 99%

“…MSCs obtained from adipose tissue are the most studied [11], although stem cells of other origins are also used. A new perspective source of MSCs is human placental tissue [58,[61][62][63]. In general, the positive effect of preparations with the inclusion of MSCs and their secretomes of various origins on the healing of skin lesions, including burn wounds, has been shown.…”

Section: Table 1 Examples Of Experimental Wound Healing Activity Stud...mentioning

confidence: 99%

From mesenchymal stem cells to their secretoms as basic components of dermal coverings for the treatment of massive burns

2023

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In the course of our long term research, we have created new biotechnological productsdermal coverings (dermis equivalents) with the inclusion of human MSCs or their secretomes (cells conditioned mediums which contain a complex of biologically active substances synthesized by them). Preclinical studies on the model animals and clinical trials of new dermal equivalents on the limited group of patients with massive burns have been conducted to determine their therapeutic effectiveness and well as safety. We proved at special in vivo experiments that new dermal coverings contribute positively to the healing severe deep burn wounds when applied to the wound surface and don't reveal any toxic effect in the studied organisms. The developed method of obtaining new biotechnological products has been patented.K e y w o r d s: cellular biotechnology, dermal covering or dermis equivalent, mesenchymal stem cell (MSC), conditioned media (CM), secretome, burns.

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“…Зараз з'являються відомості, що МСК не обов'язково повинні бути позитивними за маркером CD90 [25]. Показано наявність двох популяцій клітин С90+ і CD90-серед мультипотентних мезенхімальних стовбурових клітин плаценти [26]. У 99,6 % випадків клітини були одночасно негативні за маркерами CD45 i CD34.…”

Section: матеріали і методиunclassified

Stem potential of the new human cell line 4BL

Kushniruk¹,

Shablii²,

Shpylevaya³

et al. 2016

Visn. ukr. tov. genet. sel.

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During generation of new cell line the researchers are primarily interested in such characteristics as morphology and immunophenotype, which allows attributing beloning of the cell to that or other type and accordingly outlining branches of its application. The aim of this research was to test the stem potential of the new cell line 4BL, which was derived from the peripheral blood of healthy donor and to investigate it`s immunophenotype. Methods. The standard cell cultivation and the soft agar test were used. Stem potential was checked by differentiation in the adypogenic, osteogenic and myogenic directions. Results. The 4BL cell lines form colonies similar to embryoid bodies when grown in semisolid agar and capable to differentiate into osteogenic, myogenic and adypohenic direction when grown in induction media. Over 90 % of the 4BL line cell population expressed stem cell markers CD105 and C73 and were negative for the hemopoietic cell markers CD90, CD45, C34 and CD14. Also these cells didn’t express Oct 4. Conclusions. The new human cell line 4BL has stem potential and, most likely, belongs to non-hemopoietic multipotent stem cells.Keywords: cell line, stem cells, embryoid bodies, differentiation, flow cytometry.

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Mesenchymal and trophoblast immunophenotype of multipotent stromal cells from human placenta

Cited by 3 publications

References 10 publications

Placenta-derived multipotent cells have no effect on the size and number of DMH-induced colon tumors in rats

Placenta-derived multipotent cells have no effect on the size and number of DMH-induced colon tumors in rats

From mesenchymal stem cells to their secretoms as basic components of dermal coverings for the treatment of massive burns

Stem potential of the new human cell line 4BL

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