Merthiolate As a Preservative for Biological Products

Abstract

Introduction. Previous to the fall of 1931 this laboratory produced diphtheria toxoid by formolizing diphtheria toxin having the usual range in potency values. The minimum values of such toxin was that set forth by the National Institute of Health, namely, of an L + value of 0.2 cc. and an m.l.d. value of 0.0025 cc., and maximum values were in the range of 25 to 50 per cent greater than these. Following detoxification the toxoid was subjected to safety and antigenic tests in the usual manner. In addition to testing the toxoid undiluted, various dilutions were prepared with saline and subjected to antigenic and safety tests. It was noted that in some cases dilutions of 50 per cent or even 33⅓ per cent toxoid in saline possessed satisfactory antigenic qualities, namely, immunization of 8 out of 10 guinea pigs in six weeks against 5 m.l.d. of diphtheria toxin, the latter test being based on observation for ten days.

Experiences with a Merthiolate Bone Bank

“…Morgan, Jamieson and Powell (1933) reported the use of merthiolate solution as a preservative. O'Connor in 1939, and Brown and De Mere (1948) preserved cartilage in merthiolate.…”

“…On the other hand a merthiolate bone bank costs practically nothing, the onl y requirements being suitable containers and a supply of merthiolate. Morgan,jamieson and Powell (1933 ) reported the use ofmerthiolate solution as a preservative. O'Connor in 1939, andBrown andDe Mere (1948) preserved cartilage in merthiolate.…”