1961
DOI: 10.1152/ajplegacy.1961.200.3.545
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Abstract: Sulfobromophthalein (BSP) was injected into rats, and the livers were excised at timed intervals. The ratio of free to mercaptide-conjugated BSP in the liver was determined after extraction and chromatographic separation. After 2 minutes, 12.5% of the total dose was concentrated in the liver; of this, 65% was free BSP. At 5 minutes, 33% of the total dose appeared in the liver, of which approximately 50% was still in the free form. Increasing concentrations of BSP were infused or administered as a single inject… Show more

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Cited by 32 publications
(9 citation statements)
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“…It is currently believed that hepatic removal of BSP from plasma depends on the simultaneous operation of at least two separate processes: 1) uptake of BSP in liver cells in an amount proportional to plasma concentration, and 2) active secretion into bile (21)(22)(23)(24)(25)(26)(27)(28). Conjugation of BSP with glutathione is not obligatory for hepatic BSP uptake, since appreciable quantities of free BSP may be found in the liver within minutes after intravenous administration (29). In addition, since free BSP has been regularly identified in bile (8)(9)(10)(11)(12)(13), it is apparent that conjugation of BSP is not a prerequisite for biliary excretion.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…It is currently believed that hepatic removal of BSP from plasma depends on the simultaneous operation of at least two separate processes: 1) uptake of BSP in liver cells in an amount proportional to plasma concentration, and 2) active secretion into bile (21)(22)(23)(24)(25)(26)(27)(28). Conjugation of BSP with glutathione is not obligatory for hepatic BSP uptake, since appreciable quantities of free BSP may be found in the liver within minutes after intravenous administration (29). In addition, since free BSP has been regularly identified in bile (8)(9)(10)(11)(12)(13), it is apparent that conjugation of BSP is not a prerequisite for biliary excretion.…”
Section: Methodsmentioning
confidence: 99%
“…In addition, since free BSP has been regularly identified in bile (8)(9)(10)(11)(12)(13), it is apparent that conjugation of BSP is not a prerequisite for biliary excretion. Nevertheless, once taken up in the liver, a major portion of the BSP is metabolized; over 50 per cent of the BSP recovered in liver 5 to 20 minutes after administration was shown to be conjugated (29). Furthermore, metabolized BSP accounts for most of the BSP excreted into bile in various species (8)(9)(10)29).…”
Section: Methodsmentioning
confidence: 99%
“…BSP conjugates and free BSP are then excreted into bile. In man (5-7), the rat (6,8,9), and dog (2,5), BSP conjugates account for most of the BSP appearing in bile, approximately 75 to 85%ob in some studies. The preponderance of conjugated BSP in bile suggests that metabolized BSP is preferentially excreted.…”
mentioning
confidence: 99%
“…This makes it unlikely that diminished uptake is the cause of the increased BSP retention in newborn infants. The substantial amount of conjugated BSP often found in the serum shortly after BSP injection also suggests that uptake is unimpaired, because hepatic uptake presumably has to precede conjugation and seems to be relatively independent of this step (24). Theoretically some BSP could have been conjugated in extrahepatic tissues (25), but the enzyme activity in other organs is only a small fraction of that in the liver (26).…”
Section: Discussionmentioning
confidence: 99%
“…Why some of the BSP is conjugated prior to excretion still is not clear. There is evidence that conjugated BSP can be secreted at a higher rate than is the free dye (24). On the other hand, a mixture of BSP metabolites disappeared more slowly from the plasma than did free BSP (4).…”
Section: Discussionmentioning
confidence: 99%