Menadione stress in Saccharomyces cerevisiae strains deficient in the glutathione transferases

Castro, Frederico Augusto Vieira de; Herdeiro, R.S.; Panek, Anita D.; Eleuthério, Elis C. A.; Pereira, Marcos D.

doi:10.1016/j.bbagen.2006.10.013

Cited by 34 publications

(31 citation statements)

References 39 publications

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“…However, information on the yeast's defenses against adverse environments would shed light on its biology and assist the engineering of stress-resistant strains. This study obtained evidence for differential expression of cell rescue proteins in S. cerevisiae KNU5377 after treatment with the oxidant menadione, a quinone extensively used in studies of cellular oxidative stress (Zadzinski et al, 1998;Castro et al, 2007).…”

Section: ƍ ƍmentioning

confidence: 93%

“…ROS may also be generated by environmental changes such as heat, ethanol, hydrogen peroxide, superoxide-generating agents [e.g., menadione (MD) and paraquat], and irradiation. ROS damages a variety of cellular components such as DNA fragmentation, protein or enzyme inactivation, modification of carbohydrate compounds, and change of membrane fluidity by lipid peroxidation (Jamieson, 1998;Le Moan et al, 2006;Castro et al, 2007). In order to overcome transient or continuous ROS challenges, cells have evolved a variety of enzymatic (catalase, superoxide dismutase, thioredoxin-dependent thiol peroxidases) and non-enzymatic (trehalose) antioxidant defense systems, which are capable of protecting them from free radicals and their by-products, leading to the repair of stress-induced cellular damage, and therefore can protect the cellular constituents (MoradasFerreira and Costa, 2000;Querol et al, 2003;Fernandes et al, 2007;Herrero et al, 2008;Lopez-Mirabal and Winther, 2008).…”

Section: ƍ ƍmentioning

confidence: 99%

“…Second, antioxidant enzymes are activated during oxidative stress in S. cerevisiae (Estruch, 2000;Castro et al, 2007;Fernandes et al, 2007;Herrero et al, 2008;Lopez-Mirabal and Winther, 2008). S. cerevisiae KNU5377 cells induced a large number of antioxidant proteins involved in internal redox homeostasis (Fig.…”

Section: ƍ ƍmentioning

confidence: 99%

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Adaptive stress response to menadione-induced oxidative stress in Saccharomyces cerevisiae KNU5377

2011

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The molecular mechanisms involved in the ability of yeast cells to adapt and respond to oxidative stress are of great interest to the pharmaceutical, medical, food, and fermentation industries. In this study, we investigated the time-dependent, cellular redox homeostasis ability to adapt to menadione-induced oxidative stress, using biochemical and proteomic approaches in Saccharomyces cerevisiae KNU5377. Time-dependent cell viability was inversely proportional to endogenous amounts of ROS measured by a fluorescence assay with 2',7'-dichlorofluorescin diacetate (DCFHDA), and was hypersensitive when cells were exposed to the compound for 60 min. Morphological changes, protein oxidation and lipid peroxidation were also observed. To overcome the unfavorable conditions due to the presence of menadione, yeast cells activated a variety of cell rescue proteins including antioxidant enzymes, molecular chaperones, energy-generating metabolic enzymes, and antioxidant molecules such as trehalose. Thus, these results show that menadione causes ROS generation and high accumulation of cellular ROS levels, which affects cell viability and cell morphology and there is a correlation between resistance to menadione and the high induction of cell rescue proteins after cells enter into this physiological state, which provides a clue about the complex and dynamic stress response in yeast cells.

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Section: ƍ ƍmentioning

confidence: 93%

Section: ƍ ƍmentioning

confidence: 99%

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Adaptive stress response to menadione-induced oxidative stress in Saccharomyces cerevisiae KNU5377

2011

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“…Menadione is known to produce reactive oxygen species inside the yeast cells that can cause oxidative cell damage [23,24]. To avoid the detrimental effect and to obtain a stable electrochemical response at electrodes modified with Saccharomyces cerevisiae, menadione concentration should not exceed 100 μmol L -1 [8].…”

Section: Discussionmentioning

confidence: 99%

Mediated amperometry reveals two distinct modes of yeast responses to glucose

Garjonytė

Melvydas²,

Paškevičius³

et al. 2013

Open Life Sciences

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Mediated amperometry was exploited to monitor intracellular redox activity without cell disruption. Continuous measurements of menadione-mediated glucose currents at carbon paste electrodes with various immobilized intact wild type yeasts (Saccharomyces cerevisiae, Candida pulcherrima, Clavispora lusitaniae, Wickerhamomyces anomalus, Pichia guilliermondii, Kluyveromyces lactis var. lactis, Debaryomyces hansenii, Candida zeymolaydes and Candida tropicalis) revealed two distinct and previously unreported modes of development of the currents during the first 2 to 3 min. after subjection to glucose. A correlation among the values of the currents and the capacities of wild type yeasts to secrete various substances was observed.

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“…Menadione (MD; 2-methyl-1,4-naphtoquinone; vitamin K3) has been used as a model for studies coupled with oxidative stress (Castro et al 2007). Oxidative stress is the result of an imbalance between oxidant and antioxidant systems (Jamieson 1998).…”

Section: Introductionmentioning

confidence: 99%

Decarbonylated cyclophilin A Cpr1 protein protects KNU5377Y when exposed to stress induced by menadione

Kim

Jin

Yoon

2011

Cell Stress and Chaperones

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Cyclophilins are conserved cis-trans peptidyl-prolyl isomerase that are implicated in protein folding and function as molecular chaperones. The accumulation of Cpr1 protein to menadione in Saccharomyces cerevisiae KNU5377Y suggests a possibility that this protein may participate in the mechanism of stress tolerance. Stress response of S. cerevisiae KNU5377Y cpr1Δ mutant strain was investigated in the presence of menadione (MD). The growth ability of the strain was confirmed in an oxidant-supplemented medium, and a relationship was established between diminishing levels of cell rescue enzymes and MD sensitivity. The results demonstrate the significant effect of CPR1 disruption in the cellular growth rate, cell viability and morphology, and redox state in the presence of MD and suggest the possible role of Cpr1p in acquiring sensitivity to MD and its physiological role in cellular stress tolerance. The in vivo importance of Cpr1p for antioxidant-mediated reactive oxygen species (ROS) neutralization and chaperone-mediated protein folding was confirmed by analyzing the expression changes of a variety of cell rescue proteins in a CPR1-disrupted strain. The cpr1Δ to the exogenous MD showed reduced expression level of antioxidant enzymes, molecular chaperones, and metabolic enzymes such as nicotinamide adenine dinucleotide phosphate (NADPH)- or adenosine triphosphate (ATP)-generating systems. More importantly, it was shown that cpr1Δ mutant caused imbalance in the cellular redox homeostasis and increased ROS levels in the cytosol as well as mitochondria and elevated iron concentrations. As a result of excess ROS production, the cpr1Δ mutant provoked an increase in oxidative damage and a reduction in antioxidant activity and free radical scavenger ability. However, there was no difference in the stress responses between the wild-type and the cpr1Δ mutant strains derived from S. cerevisiae BY4741 as a control strain under the same stress. Unlike BY4741, KNU5377Y Cpr1 protein was decarbonylated during MD stress. Decarbonylation of Cpr1 protein in KNU5377Y strain seems to be caused by a rapid and efficient gene expression program via stress response factors Hsf1, Yap1, and Msn2. Hence, the decarbonylated Cpr1 protein may be critical in cellular redox homeostasis and may be a potential chaperone to menadione.

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Menadione stress in Saccharomyces cerevisiae strains deficient in the glutathione transferases

Cited by 34 publications

References 39 publications

Adaptive stress response to menadione-induced oxidative stress in Saccharomyces cerevisiae KNU5377

Adaptive stress response to menadione-induced oxidative stress in Saccharomyces cerevisiae KNU5377

Mediated amperometry reveals two distinct modes of yeast responses to glucose

Decarbonylated cyclophilin A Cpr1 protein protects KNU5377Y when exposed to stress induced by menadione

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