Membrane traffic from the endoplasmic reticulum to the Golgi apparatus is disturbed by an inhibitor of the Ca2+-ATPase in the ER

Busch, Marion B.; Sievers, Andreas

doi:10.1007/bf01403395

Cited by 18 publications

(4 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This apparent similarity between animal and plant cells further supports the notion of comparable control mechanisms for the regulation ofexo-and endocytosis among eukaryotic cells (e.g., Zorec and Tester 1992;Busch and Sievers 1993).…”

Section: Auxin Does Not Affect the CM Of Patch-clamped Protoplastssupporting

confidence: 54%

Raising the cytosolic Ca2+ concentration increases the membrane capacitance of maize coleoptile protoplasts: Evidence for Ca2+-stimulated exocytosis

Thiel

Rupnik

Zorec

1994

Planta

View full text Add to dashboard Cite

Abstract. Enhanced elongation of coleoptile cells hasbeen proposed to be related to a rise in secretory activity. Therefore, to obtain a direct measurement of exocytotic events in maize (Zea mays L.) coleoptile protoplasts we used the patch-clamp method to record changes in membrane capacitance (Cm) as a parameter proportional to fluctuations of the membrane surface area. The secretory activity of protoplasts was correlated with the cytosolic free Ca 2 + concentration ([Ca 2 +]cyt): dialyzing protoplasts with 1 laM [Ca2+]cy t caused a steady rise in Cm of 3.3_ pF-s 1. In contrast, dialysis with a solution containing <20 nM Ca 2+ produced a small and persistent decrease in Cm. This demonstrates that secretory activity in coleoptile cells can be controlled by factors which modulate [Ca2+]cy t.

show abstract

Section: Auxin Does Not Affect the CM Of Patch-clamped Protoplastssupporting

confidence: 54%

Raising the cytosolic Ca2+ concentration increases the membrane capacitance of maize coleoptile protoplasts: Evidence for Ca2+-stimulated exocytosis

Thiel

Rupnik

Zorec

1994

Planta

View full text Add to dashboard Cite

show abstract

“…Recently, Hwang et al (1997), working with cultured carrot cells, have suggested that the ER-type is mainly associated with ER and the plasma membrane type with the plasma membrane, tonoplast, and ER and that the ERtype is sensitive to CPA, whereas the plasma membrane type is not. Several lines of evidence have also indicated that Ca 2 + -ATPase is localized in the ER and sensitive to CPA in other plant species as well as carrot cells (Buckout 1984, Briskin 1990, Hsieh et al 1991, Busch and Sievers 1993, Bush and Wang 1995. The ER-type Ca 2 + -ATPase of Arabidopsis thaliana, when heterologously expressed in yeast is sensitive to CPA but insensitive to thapsigargin (Liang et al 1997).…”

Section: Discussionmentioning

confidence: 99%

Involvement of intracellular Ca²⁺ in blue light‐dependent proton pumping in guard cell protoplasts from Vicia faba

Shimazaki

Goh

Kinoshita

1999

Physiologia Plantarum

View full text Add to dashboard Cite

intracellular stores, and by 10 mM 2,5-di-(tert-butyl)-1,4-ben-Recent studies have suggested that Ca 2 + /calmodulin (CaM) or CaM-like proteins may be involved in blue light (BL)-de-zohydroquinone (BHQ) and 10 mM cyclopiazonic acid (CPA), inhibitors of Ca 2 + -ATPase in the sarcoplasmic and endoplas-pendent proton pumping in guard cells. As the increase in cytosolic concentration of Ca 2 + is required for the activation mic reticulum (ER). By contrast, the inhibitions were not observed by 10 mM thapsigargin, an inhibitor of animal of CaM and CaM-like proteins, the origin of the Ca 2 + was ER-type Ca 2 + -ATPase. The inhibitions by caffeine and BHQ investigated by measuring BL-dependent proton pumping with were reversible. Light-dependent stomatal opening in the epi-various treatments using guard cell protoplasts (GCPs) from Vicia faba. BL-dependent proton pumping was affected nei-dermis of Vicia was inhibited by caffeine, BHQ, and CPA. ther by Ca 2 + channel blockers nor by changes of Ca 2 + From these results, we conclude that the Ca 2 + thought to be concentration in the medium used for the GCPs. Addition of required for BL-dependent proton pumping may originate Ca 2 + ionophores and an agonist to GCPs did not induce from intracellular Ca 2 + stores, most likely from ER in guard cells, and that this origin of Ca 2 + may generate a stimulus-proton pumping. However, BL-dependent proton pumping was inhibited by 10 mM caffeine, which releases Ca 2 + from the specific Ca 2 + signal for stomatal opening.

show abstract

“…All treatments resulted in similar responses in terms of both cellular growth and CMTs distribution. CPA is an inhibitor of Ca 2+-ATPase in the endoplasmic reticulum (Busch and Sievers, 1993) and such a treatment is expected to deregulate the cytosolic Ca concentration essential for most cellular functions (Bush,1993). Consequently, disturbed vesicle trafficking between components of the endomembrane system, as described by Buschand Sievers (1993), might be of relevance for Ca-mediated effects on the post-mitotic cell expansion.…”

Section: Discussionmentioning

confidence: 99%

“…CPA is an inhibitor of Ca 2+-ATPase in the endoplasmic reticulum (Busch and Sievers, 1993) and such a treatment is expected to deregulate the cytosolic Ca concentration essential for most cellular functions (Bush,1993). Consequently, disturbed vesicle trafficking between components of the endomembrane system, as described by Buschand Sievers (1993), might be of relevance for Ca-mediated effects on the post-mitotic cell expansion. High external potassium concentration affects the electrophysiological properties of the plasma membrane (Spanswick, 1970).…”

Section: Discussionmentioning

confidence: 99%

Root Cap Mucilage and Extracellular Calcium as Modulators of Cellular Growth in Postmitotic Growth Zones of the Maize Root Apex*

et al. 1996

View full text Add to dashboard Cite

The control of maize root growth by root cap mucilage and extracellular calcium (Ca) was examined. Special attention was paid to the influence of these factors on cellular aspects of root growth, such as cell shape and organization of the microtubular (MT) cytoskeleton. Externally supplied Ca impaired the transition of early post-mitotic cells from a more-or-Iess apolar mode of expansion to a strictly anisotropic mode of elongation accompanied by their more rapid growth. However, this inhibitory effect of Ca was not associated with any re-arrangement of the cortical MTs, their transverse arrays, with respect to the root axis, being maintained under these conditions. Root mucilage, collected from donor root caps and placed around root tips, exerted a similar effect on cell shapes as did externally supplied Ca. In contrast, roots grown in a medium of low Ca content, or from which the root cap mucilage was continually removed, had more elongated cell shapes in their post-mitotic growth regions when compared to the control roots. These findings are consistent with a notion that Ca is present in the root cap mucilage in physiologically relevant amounts and can mediate growth responses in both the PIG region and the apical part of the elongation zone.Integrating several known effects of Ca ions on growth at the root apex, a hypothesis is proposed that a Ca-mediated and MTindependent control of cell growth in the PIG region might be involved in morphogenetic root movements (e.g. gravitropism), and that root growth responses could be initiated by an asymmetric distribution of extracellular calcium, or root cap slime, around the growing root tip.

show abstract

Membrane traffic from the endoplasmic reticulum to the Golgi apparatus is disturbed by an inhibitor of the Ca2+-ATPase in the ER

Cited by 18 publications

References 34 publications

Raising the cytosolic Ca2+ concentration increases the membrane capacitance of maize coleoptile protoplasts: Evidence for Ca2+-stimulated exocytosis

Raising the cytosolic Ca2+ concentration increases the membrane capacitance of maize coleoptile protoplasts: Evidence for Ca2+-stimulated exocytosis

Involvement of intracellular Ca²⁺ in blue light‐dependent proton pumping in guard cell protoplasts from Vicia faba

Root Cap Mucilage and Extracellular Calcium as Modulators of Cellular Growth in Postmitotic Growth Zones of the Maize Root Apex*

Contact Info

Product

Resources

About

Membrane traffic from the endoplasmic reticulum to the Golgi apparatus is disturbed by an inhibitor of the Ca2+-ATPase in the ER

Cited by 18 publications

References 34 publications

Raising the cytosolic Ca2+ concentration increases the membrane capacitance of maize coleoptile protoplasts: Evidence for Ca2+-stimulated exocytosis

Raising the cytosolic Ca2+ concentration increases the membrane capacitance of maize coleoptile protoplasts: Evidence for Ca2+-stimulated exocytosis

Involvement of intracellular Ca2+ in blue light‐dependent proton pumping in guard cell protoplasts from Vicia faba

Root Cap Mucilage and Extracellular Calcium as Modulators of Cellular Growth in Postmitotic Growth Zones of the Maize Root Apex*

Contact Info

Product

Resources

About

Involvement of intracellular Ca²⁺ in blue light‐dependent proton pumping in guard cell protoplasts from Vicia faba