Membrane proteins of Mycoplasma bovis and their role in pathogenesis

Adamu, JY; Wawegama, Nadeeka K.; Browning, Glenn F.; Markham, Philip F.

doi:10.1016/j.rvsc.2013.05.016

Cited by 24 publications

(19 citation statements)

References 44 publications

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“…in adult ones (Adamu et al, 2013). Drop in milk production and calf mortality resulting from the disease produces huge economic impact to the dairy industry.…”

Section: Introductionmentioning

confidence: 99%

Effect of Mycoplasma bovis on Production of Pro-Inflammatory Cytokines by Peripheral Blood Mononuclear Cells

Valsala¹,

Rana²,

Remesh³

et al. 2017

Adv. Anim. Vet. Sci.

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| Mycoplasma bovis is an important bovine pathogen associated with calf pneumonia, mastitis, arthritis and sepicemia. It is known to persist in phagocytic and nonphagocytic cells and disseminate to various organs causing systemic infection. In this study, the effect of live M. bovis on pro-inflammatory cytokine production by bovine peripheral blood mononuclear cells was assessed by relative quantification. Temporal change in cytokine levels was assessed at 8 hourly intervals. IL-6 was upregulated (9.32 fold at 24h) by live M. bovis at MOI of 1: 10, while TNF-α levels remained unchanged over time. IL-1 and TNF-α levels were not altered by M. bovis as compared to the control group. This showed that pro-inflammatory cytokines are not much upregulated at low multiplicity of infection.

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“…in adult ones (Adamu et al, 2013). Drop in milk production and calf mortality resulting from the disease produces huge economic impact to the dairy industry.…”

Section: Introductionmentioning

confidence: 99%

Effect of Mycoplasma bovis on Production of Pro-Inflammatory Cytokines by Peripheral Blood Mononuclear Cells

Valsala¹,

Rana²,

Remesh³

et al. 2017

Adv. Anim. Vet. Sci.

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“…The specific mechanisms involved in these processes is still incompletely understood (van der Merwe et al, 2010). The pathogenicity of M. bovis is highly dependent on its cytoplasmic membrane, and in particular the proteins within or tethered to the outer surface of it (Adamu et al, 2013). Variation in mycoplasma membrane proteins affords M. bovis antigenic plasticity and assists in evasion of the host immune response to infection.…”

Section: Mycoplasma Bovis Pathogenicity and Virulence Factorsmentioning

confidence: 99%

“…Variation in surface proteins is a virulence attribute that allows M. bovis to rapidly adapt to the host environment and evade the developing host immune response (Quinn et al, 2011). Mycoplasma membrane proteins influence cell shape, cell division, motility and adhesion to host cells and are thought to be integrally involved in the pathogenesis of MbAD (Adamu et al, 2013).…”

Section: Antigenic Variation Of Membrane Proteinsmentioning

confidence: 99%

“…Aside from the Vsps, there are few other M. bovis membrane proteins that have been identified and as extensively characterised (Adamu et al, 2013). The M. bovis type strain PG45 membrane protein pMB67 was first described by Behrens et al (1996).…”

Section: Membrane Protein Pmb67mentioning

confidence: 99%

“…The protein was found to share the conserved selective lipoproteinassociated motifs of the MALP-related lipoproteins (Lysnyansky et al, 2008). The associated P68 gene was only detected in some strains of M. bovis, all of which also contained the P48 gene (Adamu et al, 2013).…”

Section: Lipoprotein P68mentioning

confidence: 99%

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Mycoplasma bovis in Australian feeder cattle

Schibrowski¹

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Mycoplasma bovis can be a commensal bacterial inhabitant of the upper respiratory tract of healthy bovines. However, under certain circumstances and conditions that are incompletely understood, M. bovis can be associated with a number of clinical syndromes, one of which is bovine respiratory disease (BRD). In Australia, there is little information on the prevalence and epidemiology M. bovis in beef production systems. The overall aim of this thesis was to help close this knowledge gap.Commercially available serological diagnostic methods for the detection of M. bovis-specific antibody have not been validated in the Australian cattle population. Arguably, more importantly, however is the fact that diagnostic sensitivity (Se) and specificity (Sp) and / or analytical sensitivity (ASe) and specificity (ASp) estimates for commercially available M. bovis sero-diagnostic tests are not available. 28% (95% CI: 1% -92%). However, in terms of Sp the commercial ELISAs out performed western blotting; BioK302: 96% (95% CI: 87% -99%); BioK260:100% (95% CI: 93% -100%) and western blotting: 88% (95% CI: 56% -98%) respectively. In terms of both Se and Sp combined, none of the methods assessed here performed well. As such, when interpreting the results obtained from these ELISAs, the potential impact imperfect Se and / or Sp can have needs to be considered.Despite the poor performance of the BioK302 and BioK260 ELISAs particularly in terms of Se, these tests were used to determine the sero-prevalence of M. bovis antibody in Australian feeder cattle at feedlot induction and draft (approximately 42 days on feed). Paired bovine sera from 1,354 randomly selected animals were sourced from a bovine serum bank (n = 35,160) collected as part of another study, The National Bovine Respiratory Disease Initiative. As the classification of the result of a single serum sample could differ between the BioK302 and BioK260, sero-prevalence estimates were obtained using the individual ELISA results in addition to series and parallel interpretation of the two ELISA kit results. The apparent sero-prevalence of M. bovis-specific antibody at feedlot induction estimates ranged from 2.4% -6.8% (95% CI: 1.3% -8.6%) and the apparent sero-prevalence estimates at draft ranged from 22.4% -44.2% (95% CI: 19.1% -48.6%). The level of sero-conversion to M. bovis between induction and draft was also determined. Only animals that were classified as sero-negative at induction were included in sero-conversion analyses. In the current study, 24.3% (95% iii CI: 20.8% -27.7%) and 19.5% (95% CI: 17.3% -21.7%) of animals sero-converted to M. bovis between induction and draft based on the BioK302 and dichotomised BioK260 ELISA results respectively.Using these ELISA results, an assessment of putative risk factors associated with sero-positivity at feedlot induction or sero-conversion between induction and draft was performed. An increased risk of sero-positivity at feedlot induction was found to be associated with; (i) history of exposure to a saleyard at least 27 d...

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Binding of CD14 to Mycoplasma genitalium-Derived Lipid-Associated Membrane Proteins Upregulates TNF-α

Wang

Zeng

et al. 2013

Inflammation

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Lipid-associated membrane proteins (LAMPs) are a mixture of mycoplasmal lipoproteins expressed on the surface, and they are the main structures for interaction with the host cells. The objective of this study was to explore the role of CD14 in immune recognition of Mycoplasma genitalium-derived LAMPs and investigate whether the binding of CD14 to LAMPs affects the inflammatory response. Enzyme-linked immunosorbent assay (ELISA), transient co-transfection, dual-luciferase reporter assay, specific inhibition assay, and competitive inhibition ELISA (CI-ELISA) were used. CD14 was involved in LAMP-stimulated production of tumor necrosis factor (TNF)-α by blocking CD14 antibody in THP-1 cells. Co-transfection experiments in HeLa cells provide evidence that CD14 facilitates LAMP-induced TNF-α release via toll-like receptor 2 (TLR2). In addition, LAMP-induced TNF-α release was increased by soluble CD14 but decreased by soluble TLR2. Lipid moieties of LAMPs pre-treated with lipoprotein lipase were responsible for TNF-α production. The binding of CD14 to LAMPs was supported by binding assay and CI-ELISA. Thus, we provide evidences that CD14 is not only able to recognize LAMPs but also its binding to LAMPs upregulates TNF-α release. These findings provide insight into the function of CD14 and the pathogenesis of mycoplasmal infections.

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Membrane proteins of Mycoplasma bovis and their role in pathogenesis

Cited by 24 publications

References 44 publications

Effect of Mycoplasma bovis on Production of Pro-Inflammatory Cytokines by Peripheral Blood Mononuclear Cells

Effect of Mycoplasma bovis on Production of Pro-Inflammatory Cytokines by Peripheral Blood Mononuclear Cells

Mycoplasma bovis in Australian feeder cattle

Binding of CD14 to Mycoplasma genitalium-Derived Lipid-Associated Membrane Proteins Upregulates TNF-α

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