Membrane Perturbation Action Mode and Structure-Activity Relationships of Protonectin, a Novel Antimicrobial Peptide from the Venom of the Neotropical Social Wasp Agelaia pallipes pallipes

Wang, Kairong; Wen, Dandan; Yan, Jiexi; Chen, Ru; Liu, Xin; Yan, Wenjin; Zhang, Bangzhi; Xie, Junqiu; Zhang, Jindao; Wang, Rui

doi:10.1128/aac.02311-12

Cited by 40 publications

(25 citation statements)

References 38 publications

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“…From the results of our CD measurements we conclude that membrane-rupturing activity of a peptide seems to be more likely with a higher inducible helix content. [34] This finding is in agreement with previous studies, [35] in particular with work by Bechinger and co-workers, who used solid-state NMR spectroscopy to observe helix formation and orientation of some AMPs upon contact with lipid bilayers. [36] Other biophysical studies confirmed differential interaction of amphipathic AMPs with zwitterionic versus anionic lipids.…”

supporting

confidence: 90%

Piloting the Membranolytic Activities of Peptides with a Self‐organizing Map

Lin¹,

Hiss²,

Schneider³

et al. 2014

ChemBioChem

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Antimicrobial peptides (AMPs) show remarkable selectivity toward lipid membranes and possess promising antibiotic potential. Their modes of action are diverse and not fully understood, and innovative peptide design strategies are needed to generate AMPs with improved properties. We present a de novo peptide design approach that resulted in new AMPs possessing low-nanomolar membranolytic activities. Thermal analysis revealed an entropy-driven mechanism of action. The study demonstrates sustained potential of advanced computational methods for designing peptides with the desired activity.

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supporting

confidence: 90%

Piloting the Membranolytic Activities of Peptides with a Self‐organizing Map

Lin¹,

Hiss²,

Schneider³

et al. 2014

ChemBioChem

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“…It has potent antimicrobial activity against both gram positive and negative bacteria, but weak hemolytic activity [13,14]. However, the effect of protonectin on fungi cells has not been reported.…”

Section: Introductionmentioning

confidence: 97%

Antimicrobial peptide protonectin disturbs the membrane integrity and induces ROS production in yeast cells

Wang

Wen

Xie

et al. 2015

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Candidiasis is often observed in immunocompromised patients and is the 4th most common cause of bloodstream infections. However, antifungals are limited, so novel antifungal agents are urgently needed. Antimicrobial peptides (AMPs) are considered as potential alternatives of conventional antibiotics. In the present study, antimicrobial peptide protonectin was chemically synthesized and its antifungal activity and mode of action were studied. Our results showed that protonectin has potent antifungal activity and fungicidal activity against the tested fungi cells. Its action mode involved the disruption of the membrane integrity and the inducing of the production of cellular ROS. Furthermore, protonectin could inhibit the formation of biofilm and kill the adherent fungi cells. In conclusion, with the increase of fungal infection, protonectin may offer a new strategy and be considered as a potential therapeutic agent against fungal disease.

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“…aureus membrane structure, as well loss of cell-to-cell adherence. Other Hymenopteraisolated antimicrobial peptides such as melittin , polybia-MPI (Wang et al 2013b) polybia-CP (Wang et al 2012) and protonectin (Wang et al 2013a) also show important effects on microbial membranes.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial effect of Dinoponera quadriceps (Hymenoptera: Formicidae) venom against Staphylococcus aureus strains

et al. 2014

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Aims: Dinoponera quadriceps venom (DqV) was examined to evaluate the antibacterial activity and its bactericidal action mechanism against Staphylococcus aureus. Methods and Results: DqV was tested against a standard strain of methicillinsensitive Staphylococcus aureus (MSSA), Staph. aureus ATCC 6538P and two standard strains of methicillin-resistant Staphylococcus aureus (MRSA), Staph. aureus ATCC 33591 and Staph. aureus CCBH 5330. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), the rate of kill and pH sensitivity of the DqV were determined by microdilution tests. Bactericidal and inhibitory concentrations of DqV were tested to check its action on Staph. aureus membrane permeability and cell morphology. The MIC and MBC of DqV were 6Á25 and 12Á5 lg ml À1 forStaph. aureus ATCC 6538P, 12Á5 and 50 lg ml À1 for Staph. aureus CCBH 5330 and 100 and 100 lg ml À1 for Staph. aureus ATCC 33591, respectively.Complete bacterial growth inhibition was observed after 4 h of incubation with the MBC of DqV. A lowest MIC was observed in alkaline pH. Alteration in membrane permeability was observed through the increase in crystal violet uptake, genetic material release and morphology in atomic force microscopy. Conclusions:The results suggest antibacterial activity of DqV against Staph. aureus and that the venom acts in the cell membrane. Significance and Impact of the Study: Alteration in membrane permeability may be associated with the antimicrobial activity of hymenopteran venoms.

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Membrane Perturbation Action Mode and Structure-Activity Relationships of Protonectin, a Novel Antimicrobial Peptide from the Venom of the Neotropical Social Wasp Agelaia pallipes pallipes

Cited by 40 publications

References 38 publications

Piloting the Membranolytic Activities of Peptides with a Self‐organizing Map

Piloting the Membranolytic Activities of Peptides with a Self‐organizing Map

Antimicrobial peptide protonectin disturbs the membrane integrity and induces ROS production in yeast cells

Antimicrobial effect of Dinoponera quadriceps (Hymenoptera: Formicidae) venom against Staphylococcus aureus strains

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