Membrane permeabilization and cellular death of Escherichia coli, Listeria monocytogenes and Saccharomyces cerevisiae as induced by high pressure carbon dioxide treatment

Garcia‐Gonzalez, Linsey; Geeraerd, A.H.; Mast, Jan; Briers, Yves; Elst, Kathy; Ginneken, Luc Van; Impe, Jan Van; Devlieghere, Frank

doi:10.1016/j.fm.2009.12.004

Cited by 62 publications

(32 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the rupture of bacterial cytoplasmic membrane promotes the release of intracellular components such as potassium ions and nucleotides, which in turn diminishes bacterial ability to repair and replicate [16,17]. So far, the assays regarding the variation in bacterial membrane integrity, K + efflux, and nucleotides release have been widely used to examine the antibacterial actions of some select agents [18][19][20]. Thus, if asiatic acid could cause bacterial membrane damage and/or enhance the release of potassium ions and nucleotides, its antibacterial action could be explained.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial effects and action modes of asiatic acid

Liu¹,

Liu²,

Mong³

2015

BioMed

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Antibacterial effects and action modes of asiatic acid

Liu¹,

Liu²,

Mong³

2015

BioMed

View full text Add to dashboard Cite

“…Knowledge of the antimicrobial action of SCCO 2 is essential to define appropriate strategies to guarantee safety and stability of SCCO 2 processed foods and to further optimize process implementation and equipment design [2]. Although researchers have been devoted to unravel the microbial inactivation mechanism of SCCO 2 , there are few explanations that are well established yet and neither be generalized for all of examined microbial strains [3].…”

Section: Introductionmentioning

confidence: 99%

“…Although researchers have been devoted to unravel the microbial inactivation mechanism of SCCO 2 , there are few explanations that are well established yet and neither be generalized for all of examined microbial strains [3]. In recent years, several innovative scenarios have been proposed to reveal the antimicrobial effect induced by SCCO 2 treatment, including cell lyses due to depressurization [4], extraction of vital intracellular components, decrease of intracellular pH [5], inactivation of key enzymes [10], and cell membrane damage or modification [6].…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, several innovative scenarios have been proposed to reveal the antimicrobial effect induced by SCCO 2 treatment, including cell lyses due to depressurization [4], extraction of vital intracellular components, decrease of intracellular pH [5], inactivation of key enzymes [10], and cell membrane damage or modification [6]. Among these hypotheses, membrane modification/damage is considered as the most important effective factor of the inactivation mechanism of SCCO 2 treatment [2]. SCCO 2 can diffuse into the cellular membrane, then modify the membrane fluidity and increase the membrane permeability.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Membrane Damage Induced by Supercritical Carbon Dioxide in Rhodotorula mucilaginosa

Wang

Zhu

et al. 2013

Indian J Microbiol

View full text Add to dashboard Cite

To clarify the mechanism of microbial inactivation by supercritical carbon dioxide (SCCO 2 ), membrane damage of Rhodotorula mucilaginosa was investigated within specific pressure (10 Mpa), temperature (37°C), and treatment time (10-70 min) ranges, including cell morphological structure, membrane permeability and fluidity. SEM and TEM observations showed morphological changes in the cell envelope and intracellular organization after SCCO 2 treatment. Increase of membrane permeability was measured as increased uptake of the trypan blue dye with microscopy, and leakage of intracellular substances such as UV-absorbing materials and ions by determining the change of protein and electrical conductivity. The SCCO 2 mediated reduction in CFU ml -1 was 0.5-1 log higher at 37°C and 10 MPa for 60 min in Rose Bengal Medium containing 4 % sodium than a similar treatment in Rose Bengal Medium. Membrane fluidity analyzed by fluorescence polarization method using 1,6-diphenyl-1,3,5-hexatriene showed that the florescence polarization and florescence anisotropy of the SCCO 2 -treated cells were increased slightly and gently compared with the untreated cells. The correlation between membrane damage and death of cells under SCCO 2 was clear, and the membrane damage was a key factor induced the inactivation of cells.

show abstract

“…The pH decrease, both externally and internally of microbial cells, would induce DNA denaturation as DNA is sensitive to extreme acidic environments. Moreover, Garcia-Gonzalez et al (2010), in their analysis of membrane damage of E. coli, Listeria monocytogenes and Saccharomyces cerevisiae cells subjected to HPCD, speculate that HPCDinduced damage to nucleic acids during cell inactivation is due to the lower level of uptake of propidium iodide (PI).…”

Section: Introductionmentioning

confidence: 99%

Effects of high-pressure carbon dioxide on proteins and DNA in Escherichia coli

Liao

Zhang

et al. 2011

Microbiology

View full text Add to dashboard Cite

Protein changes in Escherichia coli, when subjected to high-pressure carbon dioxide (HPCD) at 10 MPa and 376C for 5-75 min, were assessed using the Bradford method, 2D electrophoresis (2-DE) and liquid chromatography-electrospray ionization-MS-MS (LC-ESI-MS-MS). The changes in DNA in E. coli under the same conditions were also investigated by using flow cytometry with propidium iodide and acridine orange, agarose gel electrophoresis (AGE) and the comet assay. The results showed that HPCD induced leakage loss of the proteins and DNA of E. coli as a function of treatment time. With regard to the protein changes, 182 proteins in the 2-DE profile were not found in the HPCD-treated E. coli. Among 20 selected protein spots exhibiting significant changes in intensity, 18 protein spots were identified as 15 known proteins and two as hypothetical proteins. These proteins were involved in cell composition, energy metabolism pathways, nucleic acid metabolism, global stress regulation and general metabolism. The DNA denaturation of E. coli induced by HPCD was demonstrated in this study for the first time to our knowledge, and the denaturation was enhanced by increasing treatment time. However, HPCD did not cause DNA degradation, as suggested by both AGE analysis and the comet assay.

show abstract

Membrane permeabilization and cellular death of Escherichia coli, Listeria monocytogenes and Saccharomyces cerevisiae as induced by high pressure carbon dioxide treatment

Cited by 62 publications

References 31 publications

Antibacterial effects and action modes of asiatic acid

Antibacterial effects and action modes of asiatic acid

Membrane Damage Induced by Supercritical Carbon Dioxide in Rhodotorula mucilaginosa

Effects of high-pressure carbon dioxide on proteins and DNA in Escherichia coli

Contact Info

Product

Resources

About