Membrane Location of Syntaxin-Binding Protein 1 Is Correlated with Poor Prognosis of Lung Adenocarcinoma

Abstract

Lung cancer is the leading cause of cancer-related death, and adenocarcinoma is the most common histological type of lung cancer. Syntaxin-binding protein 1 (STXBP1) is essential for exocytosis of secretory vesicles. Since exocytosis is the basic cellular process of cells, we investigated STXBP1 expression and clinical significance in lung adenocarcinoma. We performed quantitative real-time polymerase chain reaction in 20 pairs of lung adenocarcinoma and paired normal tissues, and demonstrated that the relative expression levels of STXBP1 mRNA in lung adenocarcinoma was significantly higher than those in normal lung tissues. We then carried out immunohistochemistry (IHC) to determine the expression profile of STXBP1 in 276 lung adenocarcinoma specimens, and categorized patients into subgroups with low or high STXBP1 expression, based on the IHC score. Moreover, STXBP1 expression phenotypes were categorized as membrane, cytoplasm, and mixed expression (both membrane and cytoplasm) expression. High STXBP1 protein accounted for 58.0% of all the 276 cases (160/276), and membrane, cytoplasm or mixed STXBP1 accounted for 28.75%, 25.63% and 45.63% in the 160 cases of high STXBP1 expression. The clinical significances of these phenotypes were evaluated by analyzing their correlation with clinicopathological factors, as well as their prognostic values. Consequently, the whole STXBP1 expression or membranal STXBP1 expression were correlated with poor prognosis and were independent prognostic factors of lung adenocarcinoma. The whole and membranal STXBP1 expression are independent prognostic factors of lung adenocarcinoma. STXBP1 detection is capable to help screen patients who may have poor prognosis and strengthen the adjuvant therapy more precisely.

Identification of cuproptosis and immune-related gene prognostic signature in lung adenocarcinoma

“…In addition, abnormal expression of STXBP1 also plays an important role in many diseases. It has been reported that STXBP1 is highly expressed in LUAD ( 51 ), which is different from this study’s results. This study analyzed and experimentally verified the LUAD data from TCGA and GEO databases, and the results showed that STXBP1 was lowly expressed in tumors.…”

A comparison of mRNA and circRNA expression between squamous cell carcinoma and adenocarcinoma of the lungs

“…Syntaxin binding protein 1 (STXBP1) encodes a syntaxin-binding protein, which plays a role in release of neurotransmitters via regulation of syntaxin (a transmembrane attachment protein receptor) (Rezazadeh et al, 2019). A recent article reported the relationship between STXBP1 and prognosis of LUAD (Wang et al, 2020). They observed that STXBP1 expression phenotypes could be categorized as membrane, cytoplasm, and mixed expression (both membrane and cytoplasm), revealing that entire STXBP1 phenotypes or membrane phenotypes were closely connected with poor prognosis of LUAD and considered as independent prognostic factors of LUAD.…”

Identification of an immune-related metabolic gene signature to predict possible prognosis in endometrial cancer and reveals immune landscape feature

“…STXBP1 encodes a syntaxinbinding protein that modulates the release of neurotransmitters [27]. In lung cancer, STXBP1 was found to be down-regulated but was identified as a prognosis-related gene in both lung squamous cell carcinoma (LUSC) and lung adenocarcinoma (LUAD) patients [28,29]. RPS27L is a p53-repressible target [30].…”