Membrane lipid domains and rafts: current applications of fluorescence lifetime spectroscopy and imaging

Almeida, Rodrigo F.M. de; Loura, Luís M. S.; Prieto, Manuel

doi:10.1016/j.chemphyslip.2008.07.011

Cited by 124 publications

(99 citation statements)

References 123 publications

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“…4) is consistent with the POPC/PSM/Chol ternary phase diagram. 9,40 Here, the passage from the l d + s o two-phase region of the POPC/PSM (50 : 50) mixture to the l o + l d two-phase region occurs at $ 4 mol% Chol. Conversely, in the PSM/Chol system, the decrease in C 4 upon Chol addition starts to be felt at mol% Chol > 20 and occurs over a broader mol% Chol range, as appears in curve (b) in Fig.…”

Section: Discussion Correlation Between Changes In Differential Capacmentioning

confidence: 99%

Electrochemical impedance spectroscopy and fluorescence lifetime imaging of lipid mixtures self-assembled on mercury

et al. 2010

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Monolayers and bilayers of lipid mixtures self-assembled on mercury form spontaneously gel-phase (solid ordered, s o ) and liquid-ordered (l o ) microdomains, thanks to the fluidity imparted to these films by the liquid metal support. The differential capacity of the hydrocarbon tail region of monolayers of mixtures of two lipid components of high and low transition temperature T m , increases during the transition from the liquid disordered (l d ) phase to the coexistence of l d and s o phases. Addition of cholesterol to this binary mixture causes a decrease in differential capacity. This behavior is explained by regarding the capacity as a measure of the total perimeter of the s o microdomains, due to the mismatch between these microdomains and the l d phase. Cholesterol removes this mismatch by converting the anisotropic s o microdomains into isotropic l o microdomains (rafts). This allows differential capacity measurements by electrochemical impedance spectroscopy to follow phase transitions in lipid mixtures. The coexistance of l d , l o and s o phases is confirmed by images of a distal lipid monolayer self-assembled on top of a thiolipid monolayer tethered to a mercury microcap, by using two-photon fluorescence lifetime imaging microscopy (2P-FLIM).

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Section: Discussion Correlation Between Changes In Differential Capacmentioning

confidence: 99%

Electrochemical impedance spectroscopy and fluorescence lifetime imaging of lipid mixtures self-assembled on mercury

et al. 2010

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“…These results suggest enrichment in ordered membrane regions in CerS2-compared with mock-and CerS5-transfected cells. The reason for this resides in the fact that Rho-DOPE is excluded from ordered membrane regions ( 36 ). Therefore, an increase in the fraction of these regions will lead to a reduction in the surface area for probe distribution (i.e., of disordered membrane regions), implying concomitant increase in the local probe concentration, and thus a decrease in the distance between Rho molecules.…”

Section: Infl Uence Of Sl Acyl Chain Structure On Membrane Biophysicamentioning

confidence: 99%

Changes in membrane biophysical properties induced by sphingomyelinase depend on the sphingolipid N-acyl chain

Pinto¹,

Laviad²,

Stiban³

et al. 2014

Journal of Lipid Research

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“…This property is very sensitive to microenvironmental changes due to micro-viscosity properties, ion concentrations, membrane fluidity, among others. As membrane fluidity changes with protein incorporation, it is possible to monitor protein-membrane interactions and the effects of different lipid compositions and lipid phases (de Almeida et al 2009;Stockl and Herrmann 2010). Another possible use of fluorescence microscopy is Förster Resonance Energy Transfer (FRET) (Raicu and Singh 2013), which allows the distance on the order of few nanometers to be determined between a pair of donor and acceptor fluorescent molecules in the system.…”

Section: Optical Microscopy Applications On Biomineralization Studiesmentioning

confidence: 99%

Biophysical aspects of biomineralization

et al. 2017

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During the process of endochondral bone formation, chondrocytes and osteoblasts mineralize their extracellular matrix (ECM) by promoting the synthesis of hydroxyapatite (HA) seed crystals in the sheltered interior of membranelimited matrix vesicles (MVs). Several lipid and proteins present in the membrane of the MVs mediate the interactions of MVs with the ECM and regulate the initial mineral deposition and posterior propagation. Among the proteins of MV membranes, ion transporters control the availability of phosphate and calcium needed for initial HA deposition. Phosphatases (orphan phosphatase 1, ectonucleotide pyrophosphatase/ phosphodiesterase 1 and tissue-nonspecific alkaline phosphatase) play a crucial role in controlling the inorganic pyrophosphate/inorganic phosphate ratio that allows MVmediated initiation of mineralization. The lipidic microenvironment can help in the nucleation process of first crystals and also plays a crucial physiological role in the function of MVassociated enzymes and transporters (type III sodiumdependent phosphate transporters, annexins and Na + /K + ATPase). The whole process is mediated and regulated by the action of several molecules and steps, which make the process complex and highly regulated. Liposomes and proteoliposomes, as models of biological membranes, facilitate the understanding of lipid-protein interactions with emphasis on the properties of physicochemical and biochemical processes. In this review, we discuss the use of proteoliposomes as multiple protein carrier systems intended to mimic the various functions of MVs during the initiation and propagation of mineral growth in the course of biomineralization. We focus on studies applying biophysical tools to characterize the biomimetic models in order to gain an understanding of the importance of lipid-protein and lipid-lipid interfaces throughout the process.Keywords Lipid microenvironment . Biomineralization . Matrix vesicles . Liposome . Proteoliposome . Hydroxyapatite Mineralization process and the biogenesis of matrix vesiclesMineralization of cartilage and bone occurs by a series of physicochemical and biochemical processes that together facilitate the deposition of calcium phosphate. The deposited calcium phosphate is subsequently converted into hydroxyapatite (HA) [Ca 10 (PO 4 ) 6 (OH) 2 ] in specific areas of the extracellular matrix (ECM). Various experiments have revealed the presence of HA crystals both inside and outside collagen fibrils in the ECM (McNally et al. 2012) and also within the

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Membrane lipid domains and rafts: current applications of fluorescence lifetime spectroscopy and imaging

Cited by 124 publications

References 123 publications

Electrochemical impedance spectroscopy and fluorescence lifetime imaging of lipid mixtures self-assembled on mercury

Electrochemical impedance spectroscopy and fluorescence lifetime imaging of lipid mixtures self-assembled on mercury

Changes in membrane biophysical properties induced by sphingomyelinase depend on the sphingolipid N-acyl chain

Biophysical aspects of biomineralization

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