Melatonin regulates antioxidant strategy in response to continuous salt stress in rice seedlings

Yan, Feifei; Wei, Haimin; Ding, Yanfeng; Li, Weiwei; Liu, Zhenghui; Chen, Lin; Tang, She; Ding, Chengqiang; Yu, Jiang

doi:10.1016/j.plaphy.2021.05.003

Cited by 51 publications

(33 citation statements)

References 85 publications

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“…It also increased ASA content and decreased DHA accumulation; upregulated the expression of ALM1 , OsPOX1 , OsCATC , OsAPX2, and OsMPG1 , thereby enhancing the tolerance of rice to alkaline stress. Similar results were also observed in rice ( Yan et al, 2021a ) and maize ( Chen et al, 2018 ). Therefore, MT may increase the activity of antioxidant enzymes (SOD, POD, CAT, and APX) and antioxidant (ASA) content, and reduce DHA content by upregulating antioxidase genes and antioxidant-related genes and then reducing excessive ROS accumulation.…”

Section: Discussionsupporting

confidence: 86%

“…Exogenous MT could further improve the proline content and the relative expression level of OsP5CS under alkaline stress ( Figures 7A , D ). Yan et al (2021a) found that exogenous MT treatment increased the sucrose content of rice under salt stress. However, in our study, exogenous MT treatment reduced sucrose and fructose contents in rice seedlings under alkaline stress ( Figures 7B , C ).…”

Section: Discussionmentioning

confidence: 96%

“…Melatonin (N-acetyl-5-methoxytryptamine, MT) is a naturally occurring molecule and was first discovered in plants in 1995 ( Dubbels et al, 1995 ). MT, as a plant growth regulator, not only regulates plant development but also alleviates the damage of abiotic and biotic stresses such as salt ( Yan et al, 2021a ), drought ( Fleta-Soriano et al, 2017 ; Wang et al, 2017b ), cold ( Bajwa et al, 2014 ), light and high temperature ( Tiryaki and Keles, 2012 ), heavy metals ( Li et al, 2021a ), dark- and heat-induced leaf senescence ( Zhang et al, 2016 , 2017b ), nutrient deficiency ( Kobylinska et al, 2018 ), and diseases ( Byeon et al, 2016 ) on plants. MT is not only an efficient ROS scavenger but also interacts with ROS directly.…”

Section: Introductionmentioning

confidence: 99%

“…MT is not only an efficient ROS scavenger but also interacts with ROS directly. Studies have shown that one molecule of MT can scavenge up to eight or more ROS through free radical scavenging reactions, allowing cell membrane to be stabilize and cellular oxidative stress to be reduce ( Garcia et al, 2014 ; Yan et al, 2021a ). On the other hand, by modulating the activity of antioxidant enzymes, MT can boost its antioxidant efficacy.…”

Section: Introductionmentioning

confidence: 99%

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Exogenous Melatonin Alleviates Alkaline Stress by Removing Reactive Oxygen Species and Promoting Antioxidant Defence in Rice Seedlings

Min

Shi

et al. 2022

Front. Plant Sci.

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Saline-alkali stress seriously restricts rice growth, development, and production in northern China. The damage of alkaline stress on rice is much greater than that of salt due to ion toxicity, osmotic stress, and especially high pH. As a signal molecule, melatonin (N-acetyl-5-methoxytryptamine, MT) mediates many physiological processes in rice and participates in protecting rice from abiotic stress. The potential mechanism of exogenous melatonin-mediated alkaline stress tolerance is still largely unknown. In this study, the effects of melatonin on the morphological change, physiological property, and corresponding genes expression in rice seedlings were analyzed under alkaline stress (20 mmol L−1, pH 9.55). The results showed that the expression levels of MT synthesis genes (TDC2, T5H, SNAT, ASMT1, and ASMT2) were induced by both exogenous MT and alkaline stress treatment. The cell membrane was protected by MT, and the MT furtherly play role in scavenging reactive oxygen species (ROS), reducing lipoxygenase (LOX) activity, and malondialdehyde (MDA) content. The scavenging of ROS by melatonin is attributed to the coupling of the improvement of redox homeostasis and the enhancement of antioxidant enzyme activity and antioxidant content by upregulating the transcriptional levels of antioxidase genes. In the meantime, MT pretreatment promoted the accumulation of free proline, sucrose, and fructose by regulating the OsP5CS, OsSUS7, and OsSPS1 gene expression level and increased chlorophyll content upregulating the expression of chlorophyll synthesis-related genes. Ultimately, the alleviating effect of exogenous melatonin on alkaline stress was reflected in increasing the leaf relative water content (RWC) and root-shoot ratio and reducing the leaf tip wilt index (TWI) through a series of physiological and biochemical changes. Melatonin pretreatment changed the expression level of MT synthesis genes which might contribute to MT synthesis in rice, consequently, activated the ROS scavenging system and alleviating the damage of alkaline stress on rice seedlings. Our study comprehensively understands the alleviating effect of exogenous melatonin on rice under alkaline stress.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Exogenous Melatonin Alleviates Alkaline Stress by Removing Reactive Oxygen Species and Promoting Antioxidant Defence in Rice Seedlings

Min

Shi

et al. 2022

Front. Plant Sci.

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“…The HKT gene family [77], SNRK gene family [78], SPL gene family [79] and PGDH gene family [80], among others, have been identified to regulate salt stress. Correlational research has presented considerable progress in exploring the salt responses of major plants, such as Arabidopsis [81][82][83], soybean [84][85][86], rice [87][88][89] and maize [90][91][92]. Plant breeders and biotechnologists have tried to develop crop varieties with a strong capacity to respond to salt stress, mainly including improving the plant's salt tolerant and salt resistance.…”

Section: Discussionmentioning

confidence: 99%

Key Cannabis Salt-Responsive Genes and Pathways Revealed by Comparative Transcriptome and Physiological Analyses of Contrasting Varieties

Zhang

Huang

et al. 2021

Agronomy

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For the dissection and identification of the molecular response mechanisms to salt stress in cannabis, an experiment was conducted surveying the diversity of physiological characteristics. RNA-seq profiling was carried out to identify differential expression genes and pathway which respond to salt stress in different cannabis materials. The result of physiological diversity analyses showed that it is more sensitive to proline contents in K94 than in W20; 6 h was needed to reach the maximum in K94, compared to 12 h in W20. For profiling 0–72 h after treatment, a total of 10,149 differentially expressed genes were identified, and 249 genes exhibited significantly diverse expression levels in K94, which were clustered in plant hormone signal transduction and the MAPK signaling pathway. A total of 371 genes showed significant diversity expression variations in W20, which were clustered in the phenylpropanoid biosynthesis and plant hormone signal transduction pathway. The pathway enrichment by genes which were identified in K94 and W20 showed a similar trend to those clustered in plant hormone signal transduction pathways and MAPK signaling. Otherwise, there were 85 genes which identified overlaps between the two materials, indicating that these may be underlying genes related to salt stress in cannabis. The 86.67% agreement of the RNA-seq and qRT-PCR indicated the accuracy and reliability of the RNA-seq technique. Additionally, the result of physiological diversity was consistent with the predicted RNA-seq-based findings. This research may offer new insights into the molecular networks mediating cannabis to respond to salt stress.

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Transcriptome analysis of the molecular basis of 11‐eicosenoic acid‐mediated salt stress tolerance in rice

Dong,

He,

Luo

et al. 2024

Crop Science

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Salt stress is one of the major abiotic factors severely hampering rice production. Fatty acids play a crucial role in plants response to such stress. Previous studies have demonstrated the potential of unsaturated fatty acid (11‐eicosenoic acid [EA]) to enhance salt tolerance in rice, but the molecular mechanism remains unclear. Our study indicates that the exogenous application of EA could alleviate salt stress‐induced damage as well as improve the survival rate of rice seedlings. Transcriptome analysis was performed following treatment of rice seedlings with EA, NaCl, and NaCl + EA. The results showed that there were 4970, 11, 304, and 4735 differentially expressed genes (DEGs) in NaCl versus CK, EA versus CK, NaCl + EA versus NaCl, and NaCl + EA versus CK, respectively. Notably, pathways such as unsaturated fatty acid metabolism, diterpenoid phytoalexin biosynthesis, and phytohormone signal transduction were significantly enriched in NaCl + EA versus NaCl. Specifically, genes related to ethylene, abscisic acid, jasmonic acid, salicylic acid, gibberellin signaling, and fatty acid metabolism were implicated in the mitigation effect of EA on salt stress in rice. Furthermore, DEGs involved in rice diterpenoid biosynthesis were analyzed in detail, revealing that EA induced the biosynthesis of multiple diterpenoids. Metabolomic analysis showed that the levels of various fatty acids, phytohormones, and terpenes increased. The presented data may serve as a useful guide for further exploration of the various biological functions of EA in enhancing plant resilience to salt stress. Additionally, it offers a valuable list of genes for the development of crop plants with improved salt resistance.

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Melatonin regulates antioxidant strategy in response to continuous salt stress in rice seedlings

Cited by 51 publications

References 85 publications

Exogenous Melatonin Alleviates Alkaline Stress by Removing Reactive Oxygen Species and Promoting Antioxidant Defence in Rice Seedlings

Exogenous Melatonin Alleviates Alkaline Stress by Removing Reactive Oxygen Species and Promoting Antioxidant Defence in Rice Seedlings

Key Cannabis Salt-Responsive Genes and Pathways Revealed by Comparative Transcriptome and Physiological Analyses of Contrasting Varieties

Transcriptome analysis of the molecular basis of 11‐eicosenoic acid‐mediated salt stress tolerance in rice

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