2018
DOI: 10.18632/aging.101602
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Abstract: Transplantation of adipose-derived mesenchymal stem cells (ADMSCs) can aid in the treatment of numerous diseases in animals. However, natural aging during in vitro expansion of ADMSCs prior to their use in transplantation restricts their beneficial effects. Melatonin is reported to exert biorhythm regulation, anti-oxidation, and anti-senescence effects in various animal and cell models. Herein, by using a senescent canine ADMSCs (cADMSCs) cell model subjected to multiple passages in vitro, we investigated the … Show more

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Cited by 68 publications
(67 citation statements)
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References 52 publications
(59 reference statements)
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“…Leydig cells (MLTC1) were cultured in 1640 medium supplemented with 10% FBS, 2 mmol/L L-glutamine, and 1% albumin. According to previous reports [31][32][33][34] and viability assays, the final concentrations of PA (100 μmol/L), melatonin (1 μmol/L), EX527 (2 μmol/L), 4-PBA (250 μmol/L), luzindole (10 μmol/L), and BMS-582949 (10 μmol/L) were used in this study. In all experiments, the concentrations of ethanol and DMSO were diluted to below 1% (v/v).…”
Section: Cell Culture and Treatmentmentioning
confidence: 99%
“…Leydig cells (MLTC1) were cultured in 1640 medium supplemented with 10% FBS, 2 mmol/L L-glutamine, and 1% albumin. According to previous reports [31][32][33][34] and viability assays, the final concentrations of PA (100 μmol/L), melatonin (1 μmol/L), EX527 (2 μmol/L), 4-PBA (250 μmol/L), luzindole (10 μmol/L), and BMS-582949 (10 μmol/L) were used in this study. In all experiments, the concentrations of ethanol and DMSO were diluted to below 1% (v/v).…”
Section: Cell Culture and Treatmentmentioning
confidence: 99%
“…Fang et al [81] examined the effects of melatonin supplementation on canine adipose-derived mesenchymal stem cells. They found that melatonin attenuated cell senescence and reduced endoplasmic reticulum stress (ERS), both of which are characteristics of aging [82]. Another study explored the effect of treating murine MSCs with melatonin after an ischemic injury, which leads to increased cellular levels of ROS.…”
Section: The Use Of Melatonin As An Osteogenic Therapymentioning
confidence: 99%
“…We also observed that transplantation of AMSCs had a lower therapeutic efficacy for MI in mice compared with transplantation of YMSCs. Indeed, rejuvenating aged MSCs to increase their therapeutic efficacy for cardiovascular diseases has attracted a lot of attention, and several novel strategies have been explored to rejuvenate aged MSCs, including gene modification (Liang et al, 2019; Song et al, 2017) and pharmacological pretreatment (Fang et al, 2018). However, the potential mechanisms underlying MSC senescence remain unclear.…”
Section: Discussionmentioning
confidence: 99%