Melatonin alleviates PM_2.5‐induced glucose metabolism disorder and lipidome alteration by regulating endoplasmic reticulum stress

Abstract

Exposure to fine particulate matter (PM 2.5 ) was associated with an increased incidence of liver metabolic disease. Melatonin has been shown to prevent liver glucolipid metabolism disorders. However, whether melatonin could rescue PM 2.5 -induced liver metabolic abnormalities remains uncertain. This study was to evaluate the mitigating effect of melatonin on PM 2.5 -accelerated hepatic glucose metabolism imbalance in vivo and in vitro. Schiff periodic acid shiff staining and other results showed that PM 2.5 led to a decrease in hepatic glycogen reserve and an increase in glucose content, which was effectively alleviated by melatonin. Targeted lipidomics is used to identify lipid biomarkers associated with this process, including glycerolipids, glycerophospholipids, and sphingolipids. In addition, gene microarray and quantitative polymerase chain reaction analysis of ApoE −/− mice liver suggested that PM 2.5 activated the miR-200a-3p and inhibited DNAJB9, and the targeting relationship was verified by luciferase reports for the first time. Further investigation demonstrated that DNAJB9 might motivate endoplasmic reticulum (ER) stress by regulating Ca 2+ homeostasis, thus altering the protein expression of GSK3B, FOXO1, and PCK2. Meanwhile, melatonin effectively inhibited miR-200a-3p and glucose metabolism disorder. Knockout of miR-200a-3p in L02 cells revealed that miR-200a-3p is indispensable in the damage of PM 2.5 and the therapeutic effect of melatonin. In summary, melatonin alleviated PM 2.5 -induced liver metabolic dysregulation by regulating ER stress via miR-200a-3p/DNAJB9 signaling pathway. Our data provide a prospective targeted therapy for air pollution-related liver metabolism disorders.

Exposure to ambient air pollutants, serum miRNA networks, lipid metabolism, and non-alcoholic fatty liver disease in young adults

“…Accumulating evidence from work in animal and cellular models is supportive of the mechanistic framework that air pollutant exposure can alter miRNAs, thereby impacting liver fat and/or systemic lipid metabolism ( Ding et al, 2019a ; Du et al, 2022b , 2022a ; Zhu et al, 2022 ). Additional work in mouse models has demonstrated that PM 2.5 -induced hepatic steatosis and dyslipidemia may be driven by altered expression of gene pathways regulating oxidative stress and lipid metabolism ( Ding et al, 2019b ; Wang et al, 2020 ; Xu et al, 2019 ; Zheng et al, 2013 ).…”

D‐pinitol alleviates diabetic cardiomyopathy by inhibiting the optineurin‐mediated endoplasmic reticulum stress and glycophagy signaling pathway

“…We found out that OPTN overexpression significantly upregulated the expression FLNA, which was associated with the inhibition of ERS in DCM. Moreover, GSK3β and FoxO1 participate in the occurrence and development of DCM through ERS and glycophagy (Divari et al, 2020; Du et al, 2022; Wu, Lu, et al, 2023; Wu, Xu, et al, 2023). The beneficial effect of OPTN on DCM is likely through regulation of the GSK3β/FoxO1 pathway.…”

Urban Particulate Matter Triggers Meibomian Gland Dysfunction

“…UPM exposure disturbs lipid metabolism in the liver, 72 , 73 lung, 74 and even the cardiovascular system, 75 resulting in SREBP-1 activation, abnormal serum cholesterol levels, and lipid accumulation. 76 , 77 The quality and synthesis of meibum, an important actor secreted by the MG, were significantly impaired in UPM-exposed populations.…”