Media for Cultivation of Animal Cells: An Overview

Mizrahi, A.; Lazar, Arye

doi:10.1007/978-94-011-3550-4_19

Cited by 14 publications

(7 citation statements)

References 88 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This explains the prophylactic use of autoclaving the water used for preparing tissue culture solutions, or used to saturate the incubator atmosphere, that we have put into practice for all tissue culture-related technologies. Indeed, it has been demonstrated that ion-exchange filtration and chlorine treatment of water does not enable proper removal of bacteria (Mizrahi and Lazar 1988). Moreover, due to the small size of the bacteria, the filtration of sera over 0.45-μm plastic filters is an inappropriate procedure to completely remove this contaminant, the pore size of these filters following a Gaussian profile, with an upper size larger than 0.5 μm (personal communication from a manufacturer).…”

Section: Resultsmentioning

confidence: 99%

Mycobacterium avium–intracellulare contamination of mammalian cell cultures

Lelong-Rebel

Piémont

Fabre

et al. 2008

In Vitro Cell.Dev.Biol.-Animal

View full text Add to dashboard Cite

Mycobacterium avium contamination has been described as a putative contaminant of nonphagocytic mammalian cells. Screening of numerous cultured nonphagocytic mammalian cell lines revealed the presence of intracellular bacteria that were identified as M. avium-intracellulare. An extensive and critical analysis of the origin of infection, of cure protocols, and of biological manifestations in M. avium-infected cells is presented. As no tremendous visible alteration of turbidity or pH of cell culture media, and no morphological change occurred in most M. avium-infected cell cultures, detection of an infection by these bacteria is rather difficult. Recommendations are given for treatment of irreplaceable cultures and prevention of mycobacterial contamination in a tissue culture facility.

show abstract

Section: Resultsmentioning

confidence: 99%

Mycobacterium avium–intracellulare contamination of mammalian cell cultures

Lelong-Rebel

Piémont

Fabre

et al. 2008

In Vitro Cell.Dev.Biol.-Animal

View full text Add to dashboard Cite

show abstract

“…The classic example, contamination of cell lines with HeLa (NELSON-REES et al, 1981), is well known and several cell lines have been identified to either contain a subpopulation of HeLa cells or actually consist only of HeLa cells. A late example of misidentification has been reported by DIRKS et al (1999a), where the popular human endothelial cell line ECV304 was shown to be a subclone of the human bladder line T24.…”

Section: Authenticationmentioning

confidence: 99%

“…The application and use of cell cultures has seen dramatic changes in the last 120 years starting with Roux performing experiments with embryonic chick cells in 1885, the establishment of the human HeLa cell line in 1948 to the large-scale production of biopharmaceuticals in cell lines (MIZRAHI and LAZAR, 1988;LUBINIECKI, 1998). Today, thousands of animal cell lines originating from different species and tissues displaying specific characteristics are available.…”

Section: Introductionmentioning

confidence: 99%

Maintenance of Cell Cultures‐with Special Emphasis on Eukaryotic Cells

Bardouille¹

2001

Biotechnology

View full text Add to dashboard Cite

“…To provide an alternative, a number of protein‐free cell culture media have been developed recently, and their applicability for the production of biopharmaceuticals was proven for several process conditions (Mizrahi and Lazar, 1991; Kessler, 1994). Ryll et al (1990) for example, showed that the production of recombinant human interleukin‐2 (IL‐2) was possible under protein‐free conditions in hollow fiber and stirred tank bioreactors.…”

Section: Introductionmentioning

confidence: 99%

Comparison of a Production Process in a Membrane-Aerated Stirred Tank and up to 1000-L Airlift Bioreactors Using BHK-21 Cells and Chemically Defined Protein-Free Medium

et al. 2003

View full text Add to dashboard Cite

The applicability of a protein-free medium for the production of recombinant human interleukin-2 with baby hamster kidney cells in airlift bioreactors was investigated. For this purpose, a BHK-21 cell line, adapted to grow and produce in protein-free SMIF7 medium without forming spheroids in membrane-aerated bubble-free bioreactors, was used as the producer cell line. First, cultivation of the cells was established at a 20-L scale using an internal loop airlift bioreactor system. During the culturing process the medium formulation was optimized according to the specific requirements associated with cultivation of mammalian cells under protein-free conditions in a bubble-aerated system. The effects of the addition of an antifoam agent on growth, viability, productivity, metabolic rates, and release of lactate dehydrogenase were investigated. Although it was possible to establish cultivation and production at a 20-L scale without the use of antifoaming substances, the addition of 0.002% silicon-oil-based antifoaming reagent improved the cultivation system by completely preventing foam formation. This reduced the release of lactate dehydrogenase activity to the level found in bubble-free aerated stirred tank membrane bioreactors and led to a reduction in generation doubling times by about 5 h (17%). Using the optimized medium formulation, cells were cultivated at a 1000-L scale, resulting in a culture performance comparable to the 20-L airlift bioreactor. For comparison, cultivations with protein-containing SMIF7 medium were carried out at 20- and 1000-L scales. The application of protein supplements did not lead to a significant improvement in the cultivation conditions. The results were also compared with experiments performed in a bubble-free aerated stirred tank membrane bioreactor to evaluate the influence of bubbles on the investigated culture parameters. The data implied a higher metabolic activity of the cells in airlift bioreactors with a 150% higher glucose consumption rate. The results of this study clearly demonstrate the applicability of a protein-free chemically defined medium for the production of recombinant proteins with BHK cells in airlift bioreactors.

show abstract

Media for Cultivation of Animal Cells: An Overview

Cited by 14 publications

References 88 publications

Mycobacterium avium–intracellulare contamination of mammalian cell cultures

Mycobacterium avium–intracellulare contamination of mammalian cell cultures

Maintenance of Cell Cultures‐with Special Emphasis on Eukaryotic Cells

Comparison of a Production Process in a Membrane-Aerated Stirred Tank and up to 1000-L Airlift Bioreactors Using BHK-21 Cells and Chemically Defined Protein-Free Medium

Contact Info

Product

Resources

About