Mechanisms of the Escherichia coli and Enterococcus faecalis inactivation by ozone

Ersoy, Zeynep Girgin; Barışçı, Sibel; Dinç, Özge

doi:10.1016/j.lwt.2018.10.095

Cited by 66 publications

(26 citation statements)

References 27 publications

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“…A similar trend was also observed in the content of DNA and K + . Nucleic acid and protein are important in bacterial cell growth, while K + affects cell internal balance ( 15 ). These intracellular leakages can be used as makers of cell membrane disruption.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Structural Changes and Molecular Mechanism Induced by High Hydrostatic Pressure in Enterobacter sakazakii

Liao

Han

et al. 2021

Front. Nutr.

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The contamination of infant milk and powder with Enterobacter sakazakii poses a risk to human health and frequently caused recalls of affected products. This study aims to explore the inactivation mechanism of E. sakazakii induced by high hydrostatic pressure (HHP), which, unlike conventional heat treatment, is a nonthermal technique for pasteurization and sterilization of dairy food without deleterious effects. The mortality of E. sakazakii under minimum reaction conditions (50 MPa) was 1.42%, which was increased to 33.12% under significant reaction conditions (400 MPa). Scanning electron microscopy (SEM) and fluorescent staining results showed that 400 MPa led to a loss of physical integrity of cell membranes as manifested by more intracellular leakage of nucleic acid, intracellular protein and K+. Real-time quantitative PCR (RT-qPCR) analysis presents a downregulation of three functional genes (glpK, pbpC, and ompR), which were involved in cell membrane formation, indicating a lower level of glycerol utilization, outer membrane protein assembly, and environmental tolerance. In addition, the exposure of E. sakazakii to HHP modified oxidative stress, as reflected by the high activity of catalase and super oxide dismutase. The HHP treatment lowered down the gene expression of flagellar proteins (fliC, flgI, fliH, and flgK) and inhibited biofilm formation. These results determined the association of genotype to phenotype in E. sakazakii induced by HHP, which was used for the control of food-borne pathogens.

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Section: Resultsmentioning

confidence: 99%

Evaluation of Structural Changes and Molecular Mechanism Induced by High Hydrostatic Pressure in Enterobacter sakazakii

Liao

Han

et al. 2021

Front. Nutr.

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“…Zargaran et al [68] demonstrated that O 3 was effective in controlling the growth of C. albicans and that it acted in the prevention of the formation of germ tubes and decreased the formation of biofilms. O 3 acts under fungal species in a similar manner to the effect observed in viruses, promoting modifications to the genetic material [21,34,55,69], and one of the consequences is an increase in the resistance of some isolates to antifungals [68].…”

Section: Biocidal Effect Of Ozonized Tap Watermentioning

confidence: 95%

“…The antimicrobial action of O3 is related to its powerful oxidizing action. When it decomposes, this gas generates an oxidant reaction by the liberation of reactive oxygen species (ROS), including generating hydroxyl radicals (OH − ) that have a greater oxidative potential (2.83 volts) over O3 and both act in the inactivation of several microorganisms [38,42,46,54,55]. (A,B) and E. faecalis (C,D).…”

Section: Biocidal Effect Of Ozonized Tap Watermentioning

confidence: 99%

“…Therefore, it is still necessary to investigate this behavior, taking into consideration the individual characteristics of each microorganism. Factors that can be associated to the distinct presentation of these results are the amount of lipids and lipoprotein present in the cell wall of the microorganisms, as well as the interaction between them and the behavior of the different membrane proteins [55,62].…”

Section: Biocidal Effect Of Ozonized Tap Watermentioning

confidence: 99%

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Ozonized Water in Microbial Control: Analysis of the Stability, In Vitro Biocidal Potential, and Cytotoxicity

Santos

Silva

Oliveira

et al. 2021

Biology

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O3 dissolved in water (or ozonized water) has been considered a potent antimicrobial agent, and this study aimed to test this through microbiological and in vitro assays. The stability of O3 was accessed following modifications of the physicochemical parameters of water, such as the temperature and pH, with or without buffering. Three concentrations of O3 (0.4, 0.6, and 0.8 ppm) dissolved in water were tested against different microorganisms, and an analysis of the cytotoxic effects was also conducted using the human ear fibroblast cell line (Hfib). Under the physicochemical conditions of 4 °C and pH 5, O3 remained the most stable and concentrated compared to pH 7 and water at 25 °C. Exposure to ozonized water resulted in high mortality rates for Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus faecalis, and Candida albicans. Scanning electron micrograph images indicate that the effects on osmotic stability due to cell wall lysis might be one of the killing mechanisms of ozonized water. The biocidal agent was biocompatible and presented no cytotoxic effect against Hfib cells. Therefore, due to its cytocompatibility and biocidal action, ozonized water can be considered a viable alternative for microbial control, being possible, for example, its use in disinfection processes.

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“…Ozone (O 3 ) is also a strong oxidizing agent and an efficient option for pathogen inactivation ( Chang et al., 2020 ; Girgin Ersoy et al., 2019 ; Perry et al., 2019 ) as well as for decolorization, taste and odor control for water reuse ( Zhang et al., 2020d ). Its performance in disinfection processes of real secondary effluents has not only found to be quite efficient by itself but also substantially improved by synergetic coupling to hydrogen peroxide (H 2 O 2 ) or UV irradiation ( Malvestiti and Dantas, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Photo-assisted electrochemical advanced oxidation processes for the disinfection of aqueous solutions: A review

et al. 2021

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Disinfection is usually the final step in water treatment and its effectiveness is of paramount importance in ensuring public health. Chlorination, ultraviolet (UV) irradiation and ozone (O 3 ) are currently the most common methods for water disinfection; however, the generation of toxic by-products and the non-remnant effect of UV and O 3 still constitute major drawbacks. Photo-assisted electrochemical advanced oxidation processes (EAOPs) on the other hand, appear as a potentially effective option for water disinfection. In these processes, the synergism between electrochemically produced active species and photo-generated radicals, improve their performance when compared with the corresponding separate processes and with other physical or chemical approaches. In photo-assisted EAOPs the inactivation of pathogens takes place by means of mechanisms that occur at different distances from the anode, that is: (i) directly at the electrode’s surface (direct oxidation), (ii) at the anode’s vicinity by means of electrochemically generated hydroxyl radical species (quasi-direct), (iii) or at the bulk solution (away from the electrode surface) by photo-electrogenerated active species (indirect oxidation). This review addresses state of the art reports concerning the inactivation of pathogens in water by means of photo-assisted EAOPs such as photo-electrocatalytic process, photo-assisted electrochemical oxidation, photo-electrocoagulation and cathodic processes. By focusing on the oxidation mechanism, it was found that while quasi-direct oxidation is the preponderant inactivation mechanism, the photo-electrocatalytic process using semiconductor materials is the most studied method as revealed by numerous reports in the literature. Advantages, disadvantages, trends and perspectives for water disinfection in photo-assisted EAOPs are also analyzed in this work.

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Mechanisms of the Escherichia coli and Enterococcus faecalis inactivation by ozone

Cited by 66 publications

References 27 publications

Evaluation of Structural Changes and Molecular Mechanism Induced by High Hydrostatic Pressure in Enterobacter sakazakii

Evaluation of Structural Changes and Molecular Mechanism Induced by High Hydrostatic Pressure in Enterobacter sakazakii

Ozonized Water in Microbial Control: Analysis of the Stability, In Vitro Biocidal Potential, and Cytotoxicity

Photo-assisted electrochemical advanced oxidation processes for the disinfection of aqueous solutions: A review

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