Mechanisms of Plant Salt Response: Insights from Proteomics

Zhang, Heng; Han, Bing; Wang, Tai; Chen, Sixue; Li, Haiying; Zhang, Yuhong; Dai, Shaojun

doi:10.1021/pr200861w

Cited by 344 publications

(323 citation statements)

References 161 publications

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“…12B), photosynthesis-related genes involved in light harvesting, electron transfer, CO 2 assimilation, and additional mechanisms such as photosystem protein, light harvesting complex I/II binding proteins, Rubisco activase, and chlorophyll a/b-binding proteins are up-regulated and are presented as potential candidates in salt stress tolerance, as suggested by Walia et al (2007) in barley (Hordeum vulgare). Most of the photosynthesis-related genes are induced under salt stress in different species such as Arabidopsis, wheat (Triticum durum), rice, or grapevine (Vitus vinifera; for review, see Zhang et al, 2012). Different strategies can nevertheless be adopted by plants to modulate their growth under stress conditions.…”

Section: Slzf2 Modulates Salt Stress Responsementioning

confidence: 99%

The Solanum lycopersicum Zinc Finger2 Cysteine-2/Histidine-2 Repressor-Like Transcription Factor Regulates Development and Tolerance to Salinity in Tomato and Arabidopsis

et al. 2014

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The zinc finger superfamily includes transcription factors that regulate multiple aspects of plant development and were recently shown to regulate abiotic stress tolerance. Cultivated tomato (Solanum lycopersicum Zinc Finger2 [SIZF2]) is a cysteine-2/histidine-2-type zinc finger transcription factor bearing an ERF-associated amphiphilic repression domain and binding to the ACGTCAGTG sequence containing two AGT core motifs. SlZF2 is ubiquitously expressed during plant development, and is rapidly induced by sodium chloride, drought, and potassium chloride treatments. Its ectopic expression in Arabidopsis (Arabidopsis thaliana) and tomato impaired development and influenced leaf and flower shape, while causing a general stress visible by anthocyanin and malonyldialdehyde accumulation. SlZF2 enhanced salt sensitivity in Arabidopsis, whereas SlZF2 delayed senescence and improved tomato salt tolerance, particularly by maintaining photosynthesis and increasing polyamine biosynthesis, in salt-treated hydroponic cultures (125 mM sodium chloride, 20 d). SlZF2 may be involved in abscisic acid (ABA) biosynthesis/signaling, because SlZF2 is rapidly induced by ABA treatment and 35S::SlZF2 tomatoes accumulate more ABA than wild-type plants. Transcriptome analysis of 35S::SlZF2 revealed that SlZF2 both increased and reduced expression of a comparable number of genes involved in various physiological processes such as photosynthesis, polyamine biosynthesis, and hormone (notably ABA) biosynthesis/signaling. Involvement of these different metabolic pathways in salt stress tolerance is discussed.

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Section: Slzf2 Modulates Salt Stress Responsementioning

confidence: 99%

The Solanum lycopersicum Zinc Finger2 Cysteine-2/Histidine-2 Repressor-Like Transcription Factor Regulates Development and Tolerance to Salinity in Tomato and Arabidopsis

et al. 2014

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“…Proteomik sonuçlar, tuz stresine cevap ve tuza toleransın altında yatan fotosentetik işlevlerin anlaşılmasını önemli düzeyde arttırmıştır. Bu tuza-cevap proteinleri ışık reaksiyonu, CO 2 özümlemesi ve diğer fotosentezle ilişkili işlevlerin regülasyonunu kapsar [19].…”

Section: Fotosentez Ve Solunum Metabolizmasıunclassified

“…Bitkilerde ROT savunma sisteminde tuza cevapta proteinler/enzimlerin şematik gösterimi. 2-P-glikolat, 2-fosfoglikolat; 3-P-glikolat, 3-fosfoglikolat; AOX, alternatif oksidaz; APX, askorbat peroksidaz; CAT, katalaz; DHA, dehidroaskorbat; DHAR, dehidroaskorbat redüktaz; GLR, glutaredoksin; GOX, glikolat oksidaz; GPX, glutatyonperoksidaz; GR, glutatyon redüktaz; GLR, glutaredoksin; GOX, glikolat oksidaz; GPX, glutatyon peroksidaz; GR, glutatyon redüktaz; GSH, indirgenmiş glutatyon; GSSG, okside glutatyon; GST, glutatyon Stransferaz; MDA, monodehidroaskorbat; MDAR, monodehidroaskorbat redüktaz; NAD + /NADH, nikotinamid adenin dinükleotid; NADP + /NADPH, nikotinamid adenin dinükleotid fosfat; PGP, fosfoglikolat fosfotaz; PrxR, peroksiredoksin; RBOH, solunum oksidaz homologu (NADPH oksidaz); RuBisCO, ribuloz-1,5-bifosfat karboksilaz/oksijenaz; RuBP, ribuloz-1,5-bifosfat; SOD, superoksit dismutaz; Trx, tiyoredoksin (Zhang et al [19]'dan değiştirilerek).…”

Section: Rot'lara Karşı Savunma Sistemleriunclassified

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Tuz Stresi Altındaki Bitkilerin Metabolik Yollarındaki Proteom Değişimleri

Yıldız¹,

Terzi²,

Akçalı³

2014

Bitlis Eren Üniversitesi Fen Bilimleri Dergisi

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ÖzetBitkilerde tuz stresine tolerans transkriptom, proteom ve metabolom kompozisyonundaki değişikliklere eşlik eden gen ekspresyonundaki önemli değişiklikleri kapsamaktadır. Proteinler direkt olarak stres cevaplarında fonksiyon görmektedir. Bu nedenle, proteomik çalışmalar tuz stresine tolerans ve fonksiyonel proteinler arasındaki muhtemel ilişkileri açıklayabilmektedir. Tuz stresine cevapta fonksiyon gören proteinlerin teşhisi tuza toleranslı transgenik bitkilerin geliştirilmesinde önemlidir. Bu derlemede, bitkilerin tuz stresine cevaplarını analiz etmek için yapılan son proteomik çalışmalar, tuz stresine cevapta muhtemel mekanizmalar ve metabolik yollarda fonksiyon gören proteinlerin seviyesindeki değişimler tartışılmıştır.Anahtar Kelimeler: Bitki, Tuz toleransı, Proteomik. Proteom Changes in Metabolic Pathways of Plants under Salt Stress AbstractTolerance against salt stress in plants includes substantial changes in gene expression which is accompanied with changes in composition of transcriptome, metabolome and proteome. Proteins are directly involved in plant stress response. Therefore, proteomics studies can explain the possible relationships between salinity tolerance and functional proteins. The identification of the proteins that are involved in responses to salinity stress could lead to the development of transgenic plants that have an enhanced tolerance to salinity. In this review, the recent proteomics studies to the analysis of the responses of plants to salinity stress, the possible mechanisms in response to salinity stress and the changes in the level of proteins involved in metabolic pathways are discussed.

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“…Among these, salinity is one of the most critical abiotic stresses that adversely affect plant germination, growth and ultimately yield (Golldack et al, 2014). It has been estimated that 20% of cultivated lands are affected by soil salinity (Zhang et al, 2012). Furthermore, the salinized areas are increasing at a rate of 10% annually for various reasons, including low precipitation, high surface evaporation, weathering of native rocks, irrigation with saline water, and poor cultural practices.…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of long term salt responsive proteins in the halophyte Suaeda maritima

Krishnamurthi¹,

George²,

Meenakshisundram³

et al. 2017

Plant Omics

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A proteomic approach was carried out to identify proteins responsive to long term salt treatment in the halophyte Suaeda maritima. A 3-month-old S. maritima seedlings were hydroponically exposed to Modified Hoagland's treated with salt solution of 200mM were grown for a period of 14 days in a growth chamber maintained at 24 ± 3°C, 70-75% relative humidity with 14 h light (200 μmol m -2 s -1 )/10 h dark cycle. The untreated set maintained in Modified Hoagland's solution was considered as control with similar conditions. Proteins extracted from the leaves of S. maritima control and salt treated seedlings were separated by Two-Dimensional gel electrophoresis (2DE). Using PDquest software analysis, we observed ~ 50 protein spots were reproducibly detected on gels, out of which 18 differentially expressed protein spots showed at least two-fold differences on 2DE maps some of them were up-regulated and few others were down-regulated in treated compared with the control. From that, we identified 6 up-regulated protein spots shows the maximum level of fold differences and these protein spots were performed trypsin digestion and Matrix Assisted Laser Desorption Ionization -Time of Flight (MALDI-TOF) analysis. The results showed that S. maritima could withstand up to 200mM NaCl for long term period of 14 days by up regulating proteins that are mainly involved in protein transport, vesicle trafficking, heme/iron binding, protein folding and assembly, chromosome segregation, cell maintenance. Our study has identified salt responsive proteins such as RAB2B, CYP71A8, SCC3 that are not previously identified by expressed sequence tag analyses or transcriptome analyses in this species. This is the first report of proteome analysis and provides insights into the molecular mechanisms of long term salt tolerance in S. maritima.

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Mechanisms of Plant Salt Response: Insights from Proteomics

Cited by 344 publications

References 161 publications

The Solanum lycopersicum Zinc Finger2 Cysteine-2/Histidine-2 Repressor-Like Transcription Factor Regulates Development and Tolerance to Salinity in Tomato and Arabidopsis

The Solanum lycopersicum Zinc Finger2 Cysteine-2/Histidine-2 Repressor-Like Transcription Factor Regulates Development and Tolerance to Salinity in Tomato and Arabidopsis

Tuz Stresi Altındaki Bitkilerin Metabolik Yollarındaki Proteom Değişimleri

Proteomic analysis of long term salt responsive proteins in the halophyte Suaeda maritima

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