Mechanisms of Murine Lacrimal Gland Repair after Experimentally Induced Inflammation

Zoukhri, Driss; Fix, Amanda; Alroy, Joseph; Kublin, Claire L.

doi:10.1167/iovs.08-1730

Cited by 84 publications

(130 citation statements)

References 34 publications

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“…Although several studies have sought to identify progenitor pools in the lacrimal gland, this is the first study to directly identify progenitors in healthy uninjured tissues (Gromova et al, 2016;You et al, 2011;Zoukhri et al, 2008). Contrary to predictions from the literature and the progenitor potential of myoepithelial cells in other organs, we found no evidence for the capacity of the myoepithelial lineage to contribute to other epithelial lineages in adult lacrimal glands (Makarenkova and Dartt, 2015;Prater et al, 2014;Zoukhri et al, 2007Zoukhri et al, , 2008.…”

Section: Discussioncontrasting

confidence: 99%

“…Previous studies have implicated myoepithelial cells as epithelial progenitors, but genetic lineage tracing of these cells has not been reported (Makarenkova and Dartt, 2015;Prater et al, 2014;Zoukhri et al, 2008). We genetically labeled myoepithelial cells in adult RFP reporter mice using the Acta2…”

Section: Novel Insights Into Myoepithelial Cell Differentiation and Omentioning

confidence: 99%

“…Furthermore, the progenitors that maintain these cell types have yet to be discovered. Over the past decade, several studies have attempted to identify lacrimal gland stem cells in order to find strategies to harvest these populations for the regeneration of the adult lacrimal gland (Gromova et al, 2016;Makarenkova and Dartt, 2015;You et al, 2011;Zoukhri et al, 2008). Although these studies suggest the presence of stem cell populations in the lacrimal gland, to date, there is still no direct in vivo evidence of a stem cell population under healthy conditions.…”

Section: Introductionmentioning

confidence: 99%

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Defining epithelial cell dynamics and lineage relationships in the developing lacrimal gland

et al. 2017

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The tear-producing lacrimal gland is a tubular organ that protects and lubricates the ocular surface. The lacrimal gland possesses many features that make it an excellent model in which to investigate tubulogenesis, but the cell types and lineage relationships that drive lacrimal gland formation are unclear. Using single-cell sequencing and other molecular tools, we reveal novel cell identities and epithelial lineage dynamics that underlie lacrimal gland development. We show that the lacrimal gland from its earliest developmental stages is composed of multiple subpopulations of immune, epithelial and mesenchymal cell lineages. The epithelial lineage exhibits the most substantial cellular changes, transitioning through a series of unique transcriptional states to become terminally differentiated acinar, ductal and myoepithelial cells. Furthermore, lineage tracing in postnatal and adult glands provides the first direct evidence of unipotent KRT5 + epithelial cells in the lacrimal gland. Finally, we show conservation of developmental markers between the developing mouse and human lacrimal gland, supporting the use of mice to understand human development. Together, our data reveal crucial features of lacrimal gland development that have broad implications for understanding epithelial organogenesis.

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Section: Discussioncontrasting

confidence: 99%

Section: Novel Insights Into Myoepithelial Cell Differentiation and Omentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Defining epithelial cell dynamics and lineage relationships in the developing lacrimal gland

et al. 2017

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“…Apoptosis and autophagy are implicated in the maintenance of ocular surface integrity both in physiological and pathological conditions (Wilson 1999;Vemuganti et al 2004;Buron et al 2006;Zoukhri et al 2008). Compelling evidence indicates that apoptosis is instrumental in glaucoma, retinitis pigmentosa, cataract, retinoblastoma, retinal ischemia, diabetic retinopathy and several eye infections caused by viruses, bacteria, fungi and some protozoans (Kaneda et al 1999;Vemuganti et al 2004;Cottet and Schorderet 2009;Zhang et al 2010;Poznic M 2009;Barber et al 2011;Pinazo-Durán et al 2013).…”

Section: Discussionmentioning

confidence: 99%

Herpes simplex virus types 1 and 2 modulate autophagy in SIRC corneal cells

et al. 2014

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Autophagy and apoptosis function as important early cellular defense mechanisms in infections and other diseases. The outcome of an infection is determined by a complex interplay between the pathogenic microorganism and these intracellular pathways. To better understand the cytopathogenicity of Herpes simplex virus types 1 and 2 (HSV-1 and -2), we studied the effect of these viruses on the autophagic and apoptotic processes in the SIRC corneal cell line. Infection with the KOS strain of HSV-1 and a wild-type strain of HSV-2 enhanced autophagosome formation, triggered cytoplasmic acidification, increased LC3B lipidation and elevated the ratio of apoptotic cells. The autophagy inhibitor bafilomycin A1 triggered a significant increase in the apoptotic responses of HSV-1-and HSV-2-infected cells. Thus, both HSV types affect autophagy and apoptosis in a coordinated fashion, and autophagy plays cytoprotective role in HSV-infected cells via antagonizing apoptosis. Together these data implicate autophagy in the pathogenic mechanism of herpetic keratitis.[Petrovski G, Pásztor K, Orosz L, Albert R, Mencel E, Moe MC, Kaarniranta K, Facskó A and Megyeri K 2014 Herpes simplex virus types 1 and 2 modulate autophagy in SIRC corneal cells.

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“…These cells were found to be nestin positive, and some of them expressed α-smooth muscle actin (α-SMA), suggesting a common source of stem cells between myoepithelial cells and acinar cells in the lacrimal gland [100,101].…”

Section: Lacrimal Gland Derived Stem Cellsmentioning

confidence: 99%

Adult Gland Derived Stem Cells (Gdscs); Potentials, Hurdles and Expectations

M¹

2014

J Stem Cell Res Ther

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Mechanisms of Murine Lacrimal Gland Repair after Experimentally Induced Inflammation

Abstract: The lacrimal gland contains stem/progenitor cells capable of tissue repair after injury. Programmed cell death after injury triggers proliferation and differentiation of these cells, presumably through activation of the BMP7 pathway.

Cited by 84 publications

References 34 publications

Defining epithelial cell dynamics and lineage relationships in the developing lacrimal gland

Defining epithelial cell dynamics and lineage relationships in the developing lacrimal gland

Herpes simplex virus types 1 and 2 modulate autophagy in SIRC corneal cells

Adult Gland Derived Stem Cells (Gdscs); Potentials, Hurdles and Expectations

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