Mechanisms of COVID-19 Entry into the Cell: Potential Therapeutic Approaches based on Virus Entry Inhibition in COVID-19 Patients with Underlying Diseases

Musavi, Hadis; Abazari, Omid; Safaee, Mohamad Sadegh; Variji, Atena; Koohshekan, Bahare; Kalaki-Jouybari, Fatemeh; Barartabar, Zeinab; Hakemi, Pejman; Mahjoub, S.

doi:10.18502/ijaai.v20i1.5409

Cited by 2 publications

(2 citation statements)

References 92 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In vitro and animal models demonstrated that piperine induces toxicity, apoptosis and cell cycle arrest in various tumor cells ( 34 ). Piperine combined with anti-cancer drugs induced more cytotoxicity in human cancer cells ( 20 , 35 , 36 ). Here, we explored the cytotoxic effects of piperine combined with cisplatin and the mechanisms of their effects in breast cancer cells of MCF-7.…”

Section: Discussionmentioning

confidence: 99%

The Synergistic Combination of Cisplatin and Piperine Induces Apoptosis in MCF-7 Cell Line

Fattah¹,

Morovati²,

Niknam³

et al. 2021

ijph

Self Cite

View full text Add to dashboard Cite

Background: Piperine is a natural compound obtained from the Piper nigrum that exhibits anti-proliferative and anti-cancer activity in cancer cell lines. We analyzed the cytotoxic effect of piperine combined with cisplatin compound in the human MCF-7 breast cancer cell line and the underlying mechanism. Methods: The present in vitro study was performed on MCF-7 cell line in Jahrom University of Medical Sciences between, Jahrom, Iran from 2016 to 2017. Cultured MCF-7 cells were seeded into four groups: a control group (untreated group), a group treated with cisplatin, a group treated with piperine and a group treated with cisplatin and piperine. Cell viability was analyzed using the MTT assay method. Flow c-ytometric analysis was investigated for apoptosis. The mRNA and protein expression of the apoptotic regulators p53, Bcl-2, Bax, caspase 3 and caspase 9 were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting analysis. Results: Piperine (20 and 30 µM) in combination with cisplatin (5, 10 and 15 µM) for 24 h synergistically inhibited cell viability of MCF-7 breast cancer cells more than piperine and cisplatin used alone. Synergistic antibreast cancer activities cisplatin (5 µM) and piperine (20 µM) were via inducing apoptosis. Piperine (20 µM) and cisplatin (5 µM) for 24 h induce apoptosis strongly through reduction of Bcl-2 and increase of caspase 3, p53, caspase 9, and Bax. Conclusion: Piperine in combination with cisplatin could trigger p53-mediated apoptosis more effective than cisplatin alone in MCF-7 breast cancer cells, reducing the toxic dose of cisplatin used in cancer chemotherapy.

show abstract

Section: Discussionmentioning

confidence: 99%

The Synergistic Combination of Cisplatin and Piperine Induces Apoptosis in MCF-7 Cell Line

Fattah¹,

Morovati²,

Niknam³

et al. 2021

ijph

Self Cite

View full text Add to dashboard Cite

show abstract

“…This virus impacts the human body after recovery. The virus penetrates the host cells via angiotensin-converting enzyme-2 (ACE-2) receptors, which are found on different types of peripheral tissue in the human body, such as the kidneys, liver, lungs, and skeletal system, which may lead to disorders in multiple organs [ 2 , 3 ].…”

Section: Introductionmentioning

confidence: 99%

The Impact of Angiotensin Converting Enzyme-2 (ACE-2) on Bone Remodeling Marker Osteoprotegerin (OPG) in Post-COVID-19 Iraqi Patients

AL-Azzawi¹,

Mohammed²

2022

Cureus

View full text Add to dashboard Cite

IntroductionAs COVID-19 affects human genes in several types of peripheral tissue, numerous disorders occur after recovery. The virus enters host cells via angiotensin-converting enzyme-2 (ACE-2) receptors that affect bone remodeling, leading to osteopenia or osteoporosis, which is characterized by low bone mineral density (BMD). The adult skeleton undergoes about 10% remodeling annually, which is crucial for preventing fatigue damage and preserving calcium homeostasis. An imbalance in the rates of bone production and resorption causes bone loss. Osteoprotegerin (OPG) is one of the regulators involved in the bone remodeling mechanism, it decreases the activity of NF-B receptors that activates the receptor activators of NF-B ligand (RANKL) pathway, which maintains the bone homeostasis balance. This study aims to find out the disruption of bone homeostasis balance in Iraqi post-COVID-19 patients. Materials and methodsIt is a case-control study that includes 130 Iraqi subjects enrolled. They were divided into two groups -the first group consisted of 80 post-COVID-19 infection patients, while the second group consisted of 50 who were not infected with COVID-19. Also, the levels of ACE-2 and OPG were measured in the serum by the ELISA technique. The BMD was measured by the DEXA scan technique. ResultsThis study found that there is an effect of coronavirus infection on the bone strength measured by the Mean of the OPG level, which was found to be highly significant in the serum of post-COVID-19 patients when compared with non-COVID-19 subjects (P-value = 0.001), but the Mean of ACE-2 level was statistically nonsignificant between the two groups (P-value = 0.13). Also, the BMD of post-COVID-19 patients that was measured by DEXA scan had a statistically highly significant T-score% between the two groups. ConclusionThe current study found that there was an effect of COVID-19 on the bone remodeling mechanism, which may be causing osteopenia or osteoporosis for Iraqi subjects enrolled in the current study. Also, analyzing the OPG level in the serum could be helpful in predicting low BMD.

show abstract

Mechanisms of COVID-19 Entry into the Cell: Potential Therapeutic Approaches based on Virus Entry Inhibition in COVID-19 Patients with Underlying Diseases

Cited by 2 publications

References 92 publications

The Synergistic Combination of Cisplatin and Piperine Induces Apoptosis in MCF-7 Cell Line

The Synergistic Combination of Cisplatin and Piperine Induces Apoptosis in MCF-7 Cell Line

The Impact of Angiotensin Converting Enzyme-2 (ACE-2) on Bone Remodeling Marker Osteoprotegerin (OPG) in Post-COVID-19 Iraqi Patients

Contact Info

Product

Resources

About