Mechanisms of carbonate precipitation induced by two model bacteria

Li, Xiaofang; He, Xiaodan; Ren, Kaiyan; Dong, Hailiang; Lian, Bin

doi:10.1016/j.chemgeo.2023.121461

Cited by 5 publications

(1 citation statement)

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“…Bacteria can release extracellular enzymes and metabolites that benefit neighboring species, creating a cooperative network within the microbial community. For example, one species may produce urease enzymes that hydrolyze urea into carbonate ions and ammonia, which can be utilized by other bacterial species as nitrogen or carbon sources (Graddy et al 2021 ; Li et al 2023a ). This mutualistic interaction promotes metabolic synergy and facilitates more efficient sand cementation and MICP processes.…”

Section: Resultsmentioning

confidence: 99%

Synergistic biocementation: harnessing Comamonas and Bacillus ureolytic bacteria for enhanced sand stabilization

Rajasekar,

Zhao,

et al. 2024

World J Microbiol Biotechnol

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Biocementation, driven by ureolytic bacteria and their biochemical activities, has evolved as a powerful technology for soil stabilization, crack repair, and bioremediation. Ureolytic bacteria play a crucial role in calcium carbonate precipitation through their enzymatic activity, hydrolyzing urea to produce carbonate ions and elevate pH, thus creating favorable conditions for the precipitation of calcium carbonate. While extensive research has explored the ability of ureolytic bacteria isolated from natural environments or culture conditions, bacterial synergy is often unexplored or under-reported. In this study, we isolated bacterial strains from the local eutrophic river canal and evaluated their suitability for precipitating calcium carbonate polymorphs. We identified two distinct bacterial isolates with superior urea degradation ability (conductivity method) using partial 16 S rRNA gene sequencing. Molecular identification revealed that they belong to the Comamonas and Bacillus genera. Urea degradation analysis was performed under diverse pH (6,7 and 8) and temperature (15 °C,20 °C,25 °C and 30 °C) ranges, indicating that their ideal pH is 7 and temperature is 30 °C since 95% of the urea was degraded within 96 h. In addition, we investigated these strains individually and in combination, assessing their microbially induced carbonate precipitation (MICP) in silicate fine sand under low (14 ± 0.6 °C) and ideal temperature 30 °C conditions, aiming to optimize bio-mediated soil enhancement. Results indicated that 30 °C was the ideal temperature, and combining bacteria resulted in significant (p ≤ 0.001) superior carbonate precipitation (14–16%) and permeability (> 10− 6 m/s) in comparison to the average range of individual strains. These findings provide valuable insights into the potential of combining ureolytic bacteria for future MICP research on field applications including soil erosion mitigation, soil stabilization, ground improvement, and heavy metal remediation.

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Section: Resultsmentioning

confidence: 99%