Mechanism Underlying Specificity of Proteins Targeting Inorganic Materials

Abstract

Adhesion force analysis using atomic force microscopy clearly revealed for the first time the mechanism underlying the specific binding between a titanium surface and ferritin possessing the sequence of Ti-binding peptide in its N-terminal domain. Our results proved that the specific binding is due to double electrostatic bonds between charged residue and surface groups of the substrate. Furthermore, it is also demonstrated that the accretion of surfactant reduces nonspecific interactions, dramatically enhancing the selectivity and specificity of Ti-binding peptide.

Biological Containers: Protein Cages as Multifunctional Nanoplatforms

“…[147] It was suggested that the speci-ficity of binding was due to electrostatic interactions between peptide and surface groups of the titanium substrate. [148] This N-terminal extension is uniquely presented on the exterior surface of the ferritin cage and is readily altered by genetic manipulation.…”

Adsorption of tripeptide RGD on rutile TiO2 nanotopography surface in aqueous solution

“…Adsorption of protein on various materials has been widely studied and it has been found that factors such as electrostatic interaction, hydrophobic interaction, hydrogen bonding, VDW interaction and specific chemical interactions between protein and the adsorbent play important roles [56][57][58][59][60][61][62][63][64][65][66][67][68][69][70][71]. Short peptide sequences interact with TiO 2 surface via electrostatic interaction also [57][58][59].…”

Diffusion and reaction pathways of water near fully hydrated TiO2 surfaces from ab initio molecular dynamics

“…7,8 Understanding water adsorption on TiO 2 is the key not only to efficiently split water molecules (as made use of in hydrogen gas production 9 ) but also to optimize adsorption on, and functionalization of, TiO 2 by larger molecules as mediated via strongly bound surface waters. [10][11][12][13][14][15] On the other hand, concerns have been raised with respect to environmental safety and health when TiO 2 is used in medical implants 16 and as engineered nanoparticles in consumer products. 17 TiO 2 nanoparticles are small enough to penetrate the blood-brain barrier, 18 can aggregate in organisms and the environment, and yield a nanotoxic response by increasing the levels of intracellular reactive oxygen species, leading to apoptosis.…”