Mechanism of the Breaking of Seed Dormancy by Flower Thinning in Heracleum moellendorffii Hance

Liu, Shuya; Jiang, Xinhong; Liu, Zaimin; Cheng, Yao; Sun, Tianyu; Yu, Xiaoyan

doi:10.1007/s00344-018-9898-4

Cited by 8 publications

(4 citation statements)

References 42 publications

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“…Yu et al [63] reported reduced carbon flux into the TCA cycle in m132, an Arabidopsis DLAT mutant, which resulted in severely diminished ATP production and disrupted cell division. ACSL is a key regulator of fatty acid β-oxidation located in the peroxisome that is involved in seed dormancy release and germination processes [64]. A total of five ACSL transcripts during E.brevicornu stratification were highly expressed in the combined stratification group but weakly expressed in the low-temperature stratification (0-3) and variable-temperature stratification (4-0) groups, suggesting that combined stratification better stimulates fatty acid β-oxidation to provide energy for seed dormancy release (Figure 9B).…”

Section: Role Of Energy (Starch and Fatty Acid) Supply In The Dormanc...mentioning

confidence: 99%

Characterizing seed dormancy in Epimedium brevicornu Maxim.: Development of novel chill models and determination of dormancy release mechanisms by transcriptomics

Xiang

Dong

et al. 2023

Preprint

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Purpose: Epimedium brevicornu Maxim. is a perennial persistent C3 plant of the genus Epimedium Linn. in the family Berberaceae that exhibits severe physiological and morphological seed dormancy. We placed mature E.brevicornu seeds under nine stratification treatment conditions and explored the mechanisms of influence by combining seed embryo growth status assessment with related metabolic pathways and gene co-expression analysis. Results: We identified 3.9 °C as the optimum cold-stratification temperature of E.brevicornu seeds via a chilling unit (CU) model. The best treatment was variable-temperature stratification (10/20 °C, 12/12 h) for 120 d followed by low-temperature stratification (4 °C) for 90 d. A total of 63801 differentially expressed genes were annotated to 2587 transcription factors (TFs) in 17 clusters in two comparisons. Genes specifically highly expressed in the dormancy release treatment group were significantly enriched in embryo development ending in seed dormancy and fatty acid degradation, indicating the importance of these two processes. Coexpression analysis implied that the TF GRF had the most reciprocal relationships with genes, and multiple interactions centered on zf-HD and YABBY as well as on MYB, GRF, and TCP were observed. Conclusion: In this study, analyses of plant hormone signal pathways and fatty acid degradation pathways revealed changes in key genes during the dor- mancy release of E.brevicornu seeds, providing evidence for the filtering of E.brevicornu seed dormancy-related genes.

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Section: Role Of Energy (Starch and Fatty Acid) Supply In The Dormanc...mentioning

confidence: 99%

Characterizing seed dormancy in Epimedium brevicornu Maxim.: Development of novel chill models and determination of dormancy release mechanisms by transcriptomics

Xiang

Dong

et al. 2023

Preprint

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“…Seed dormancy is defined as the failure of an intact viable seed to complete germination in a specified period of time under any combination of normal physical environmental factors (temperature, light/dark, and water) (Finch‐Savage and Leubner‐Metzger 2006; Chen et al 2023). Seed dormancy is an adaptive trait that evolved to enhance seedling survival by avoiding germination under unsuitable environmental conditions (Liu et al 2019a; Iwasaki et al 2022). Dormancy varies between species, clades, communities, and regions and is regulated by both internal and external factors (Zacchello et al 2022; Pausas and Lamont 2022).…”

Section: Introductionmentioning

confidence: 99%

Ascorbic acid releases dormancy and promotes germination by an integrated regulation of abscisic acid and gibberellin in Pyrus betulifolia seeds

Niu,

Xu,

Zong

et al. 2024

Physiologia Plantarum

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Seed dormancy is an important life history state in which intact viable seeds delay or prevent germination under suitable conditions. Ascorbic acid (AsA) acts as a small molecule antioxidant, and breaking seed dormancy and promoting subsequent growth are among its numerous functions. In this study, a germination test using Pyrus betulifolia seeds treated with exogenous AsA or AsA synthesis inhibitor lycorine (Lyc) and water absorption was conducted. The results indicated that AsA released dormancy and increased germination and 20 mmol L−1 AsA promoted cell division, whereas Lyc reduced germination. Seed germination showed typical three phases of water absorption; and seeds at five key time points were sampled for transcriptome analysis. It revealed that multiple pathways were involved in breaking dormancy and promoting germination through transcriptome data, and 12 differentially expressed genes (DEGs) related to the metabolism and signal transduction of abscisic acid (ABA) and gibberellins (GA) were verified by subsequent RT‐qPCR. For metabolites, exogenous AsA increased endogenous AsA and GA3 but reduced ABA and the ABA/GA3 ratio. In addition, three genes regulating ABA synthesis were downregulated by AsA, while five genes mediating ABA degradation were upregulated. Taken together, AsA regulates the pathways associated with ABA and GA synthesis, catalysis, and signal transduction, with subsequent reduction in ABA and increase in GA and further the balance of ABA/GA, ultimately releasing dormancy and promoting germination.

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“…Studies on Heracleum moellendorffii Hance seeds have shown that high expression of enoyl-CoA hydratase promotes the accumulation of fatty acids in the seeds. This facilitates the development of the embryo, and, therefore, accelerates the release of dormancy [23]. Comparative metabolomic studies of wheat seeds at two levels of dormancy have shown that unsaturated fatty acid analogs, such as cis-vaccenate, oleate, linoleate, and linolenate, undergo accumulation mediated by phospholipase A2 to maintain dormancy.…”

Section: Introductionmentioning

confidence: 99%

Dormancy release of seeds of Podophyllum hexandrum Royle accompanied by changes in phytochemicals and inorganic elements

Jiu,

Chen,

et al. 2023

PLoS ONE

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Podophyllum hexandrum Royle is an alpine medicinal plant of considerable importance, and its seed dormancy severely inhibits population renewal. Although cold stratification can break dormancy to a certain extent, the migration and accumulation of phytochemicals and inorganic elements in the seeds during dormancy release and their functions remain unclear. Changes in phytochemicals and inorganic elements in different seed parts were analyzed during dormancy. The key differential phytochemicals and inorganic elements were screened and their association with dormancy release and their roles in dormancy release were explored. The results showed that dormancy release may have occurred following the decrease in palmitic acid and linoleic acid content in the seeds and the increase in 2,3-dihydro-3,5-dihydro-6-methyl-4 (h)-pyran-4-one content in the endosperm. Meanwhile, 6-propyltridecane and hexadecane in the seed coat may enhance the water permeability of seeds to speed up germination. Mg may migrate from the seed coat to the endosperm and seed embryos, whereas Co may migrate from the seed embryo to the seed coat. Ca, Mn, Mg, and Co are involved in various physiological metabolic processes, which may facilitate the dormancy release of P. hexandrum seeds. These findings have enhanced our understanding of the mechanisms of dormancy release in P. hexandrum seeds and can serve as a reference for the development of more effective dormancy-breaking techniques for the conservation of this endangered medicinal plant.

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Mechanism of the Breaking of Seed Dormancy by Flower Thinning in Heracleum moellendorffii Hance

Cited by 8 publications

References 42 publications

Characterizing seed dormancy in Epimedium brevicornu Maxim.: Development of novel chill models and determination of dormancy release mechanisms by transcriptomics

Characterizing seed dormancy in Epimedium brevicornu Maxim.: Development of novel chill models and determination of dormancy release mechanisms by transcriptomics

Ascorbic acid releases dormancy and promotes germination by an integrated regulation of abscisic acid and gibberellin in Pyrus betulifolia seeds

Dormancy release of seeds of Podophyllum hexandrum Royle accompanied by changes in phytochemicals and inorganic elements

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