Cell Reports volume 3, issue 4, P1117-1127 2013 DOI: 10.1016/j.celrep.2013.03.002 View full text
Y. Paul Chang, Meng Xu, Ana Carolina Dantas Machado, Xian Jessica Yu, Remo Rohs, Xiaojiang S. Chen

Abstract: SUMMARY The DNA tumor virus Simian virus 40 (SV40) is a model system for studying eukaryotic replication. SV40 large tumor antigen (LTag) is the initiator/helicase that is essential for genome replication. LTag recognizes and assembles at the viral replication origin. We determined the structure of two multidomain LTag subunits bound to origin DNA. The structure reveals that the origin binding domains (OBDs) and Zn and AAA+ domains are involved in origin recognition and assembly. Notably, the OBDs recognize t…

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