Mechanism of Apatinib gene carried with carbon nanotube in regulating the growth and chemosensitivity of human papilloma virus through inducing AMPK/TSC2/mTOR signal pathway

Abstract

This study assesses the mechanism of Apatinib gene carried with CNT in regulating the growth and chemosensitivity of HPV through inducing AMPK/TSC2/mTOR signal pathway. SiHa cells were divided into blank set, empty carrier set, Apatinib set and Apatinib set carried with CNT randomly. Cell cycle of SiHa cells was detected through CCK-8 test, clone formation test and FCM. The mRNA presentation of Cyelin D3 was detected with Reverse Transcription-Polymerase Chain Reaction (RT-PCR). The protein expression of Cyelin D3, presentation and activation of AMPK, TSC2 and mTOR was detected with Western Blot assay. Expression of VEGFR-2 in Apatinib set and Apatinib set carried with Carbon nanotube (CNT) was reduced. The proliferative rate in Apatinib set was lower than in control set notably. The rate of clone formation in Apatinib set carried with CNT was declined notably compared with control set. The cell cycle was restrained in Apatinib set carried with CNT. The IC50 concentration of TAX in Apatinib set carried with CNT was lower than in control set. The expression of p-AMPK in Apatinib set and Apatinib set carried with CNT was elevated compared with control set. The active expression of AMPK was prompted in Apatinib set. And phosphorylation of mTOR was restrained. The growth of HPV was restrained and chemosensitivity of HPV was improved by Apatinib gene carried with CNT through inducing AMPK/TSC2/mTOR signal pathway.