Mechanism and factors influence of graphene-based nanomaterials antimicrobial activities and application in dentistry

Radhi, Asanah; Mohamad, Dasmawati; Rahman, Fatimah Suhaily Abdul; Abdullah, Abdul Manaf; Hasan, Habsah

doi:10.1016/j.jmrt.2021.01.093

Cited by 87 publications

(77 citation statements)

References 153 publications

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“…A total reduction of up to 55% was achieved for the 5 wt% GNP/PDMS surface, which was chosen for further assays, although there were no statistically significant differences between GNP loadings. These results are in line with those found in the literature, where higher concentrations of graphene derivatives have demonstrated a higher bactericidal or bacteriostatic activity [ 74 , 75 , 76 ].…”

Section: Resultssupporting

confidence: 92%

Performance of Graphene/Polydimethylsiloxane Surfaces against S. aureus and P. aeruginosa Single- and Dual-Species Biofilms

Oliveira

Gomes

et al. 2022

Nanomaterials

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The increasing incidence of implant-associated infections has prompted the development of effective strategies to prevent biofilm formation on these devices. In this work, pristine graphene nanoplatelet/polydimethylsiloxane (GNP/PDMS) surfaces containing different GNP loadings (1, 2, 3, 4, and 5 wt%) were produced and evaluated on their ability to mitigate biofilm development. After GNP loading optimization, the most promising surface was tested against single- and dual-species biofilms of Staphylococcus aureus and Pseudomonas aeruginosa. The antibiofilm activity of GNP/PDMS surfaces was determined by the quantification of total, viable, culturable, and viable but nonculturable (VBNC) cells, as well as by confocal laser scanning microscopy (CLSM). Results showed that 5 wt% GNP loading reduced the number of total (57%), viable (69%), culturable (55%), and VBNC cells (85%) of S. aureus biofilms compared to PDMS. A decrease of 25% in total cells and about 52% in viable, culturable, and VBNC cells was observed for P. aeruginosa biofilms. Dual-species biofilms demonstrated higher resistance to the antimicrobial activity of GNP surfaces, with lower biofilm cell reductions (of up to 29% when compared to single-species biofilms). Still, the effectiveness of these surfaces in suppressing single- and dual-species biofilm formation was confirmed by CLSM analysis, where a decrease in biofilm biovolume (83% for S. aureus biofilms and 42% for P. aeruginosa and dual-species biofilms) and thickness (on average 72%) was obtained. Overall, these results showed that pristine GNPs dispersed into the PDMS matrix were able to inhibit biofilm growth, being a starting point for the fabrication of novel surface coatings based on functionalized GNP/PDMS composites.

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Section: Resultssupporting

confidence: 92%

Performance of Graphene/Polydimethylsiloxane Surfaces against S. aureus and P. aeruginosa Single- and Dual-Species Biofilms

Oliveira

Gomes

et al. 2022

Nanomaterials

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“…In the presence of Gram (−) P. aeruginosa, the electrostatic repulsions occur between the negatively charged cell surface and CsA with negative charges, leading to the growth inhibition of the Gram (−) bacterial strain on the surface of fibrous membranes [56]. In addition, the literature reports that the bacteria with elongated shape (Gram −) are more disturbed compared to the ones with spherical shape (Gram +) due to the larger contact surface area [57]. It was also reported that the antibacterial activity of rGO implies both physical and chemical mechanisms of action: the physical deterioration is represented by the stress of the bacterial cell membrane that is produced due to the contact with the sharp edges of rGO nanosheets, while the oxidative stress denotes a chemical action mode, through which rGO generates reactive oxygen species (ROS) that can affect the bacteria DNA, resulting in their inhibition [53].…”

Section: Anti-biofilm Activity Of Nanofibrous Membranesmentioning

confidence: 99%

Electrospun Nanofibrous Membranes Based on Citric Acid-Functionalized Chitosan Containing rGO-TEPA with Potential Application in Wound Dressings

et al. 2022

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The present research work is focused on the design and investigation of electrospun composite membranes based on citric acid-functionalized chitosan (CsA) containing reduced graphene oxide-tetraethylene pentamine (CsA/rGO-TEPA) as materials with opportune bio-properties for applications in wound dressings. The covalent functionalization of chitosan (CS) with citric acid (CA) was achieved through the EDC/NHS coupling system and was checked by 1H-NMR spectroscopy and FTIR spectrometry. The mixtures to be electrospun were formulated by adding three concentrations of rGO-TEPA into the 1/1 (w/w) CsA/poly (ethylene oxide) (PEO) solution. The effect of rGO-TEPA concentration on the morphology, wettability, thermal stability, cytocompatibility, cytotoxicity, and anti-biofilm activity of the nanofibrous membranes was extensively investigated. FTIR and Raman results confirmed the covalent and non-covalent interactions that appeared between the system’s compounds, and the exfoliation of rGO-TEPA sheets within the CsA in the presence of PEO (CsA/P) polymer matrix, respectively. SEM analysis emphasized the nanofibrous architecture of membranes and the presence of rGO-TEPA sheets entrapped into the CsA nanofiber structure. The MTT cellular viability assay showed a good cytocompatibility with the highest level of cell development and proliferation registered for the CsA/P composite nanofibrous membrane with 0.250 wt.% rGO-TEPA. The designed nanofibrous membranes could have potential applications in wound dressings, given that they showed a good anti-biofilm activity against Gram-negative Pseudomonas aeruginosa and Gram-positive Staphylococcus aureus bacterial strains.

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“…It is shown that future applications can be nearly applied that photocatalysts for air, surface and water were available. Moreover, in medical based especially in dentistry, the graphene-based nanomaterial can be applied to eradicate microbial with good results [39]. Future manufacturing technology can potentially be combined with nanotechnology, as in additive manufacturing.…”

Section: Future Trendmentioning

confidence: 99%

Nanocomposite Material Synthesized Via Horizontal Vapor Phase Growth Technique: Evaluation and Application Perspective

Muflikhun¹,

Adi²,

Santos³

2022

21st Century Nanostructured Materials - Physics, Chemistry, Classification, and Emerging Applications in Industry, Biomedicine,

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The synthesis of nanomaterials has been reported by many researchers using different methods. One of the methods that can be used with perfect pureness and have less pollution in the synthesized materials results is the vapor phase growth technique (VPGT). Several types of nano shapes materials were reported such as nanoparticles, nanorods, nano triangular, nanosphere, and nanocrystal. The synthesis method has a fundamental process where the nanomaterials evaporated and condensed based on the temperature difference. There are three important variables, i.e., stochiometric ratio of source materials, temperature and baking time. The synthesis was occured in the quartz tube and sealed in the vacuum condition. This create the material was synthesis in pure and isolated conditions. The application of the nanomaterials synthesized via Horizontal Vapor Phase Growth (HVPG) can be implemented in anti-pathogen, anti-bacterial, gas sensing and coating applications.

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Mechanism and factors influence of graphene-based nanomaterials antimicrobial activities and application in dentistry

Cited by 87 publications

References 153 publications

Performance of Graphene/Polydimethylsiloxane Surfaces against S. aureus and P. aeruginosa Single- and Dual-Species Biofilms

Performance of Graphene/Polydimethylsiloxane Surfaces against S. aureus and P. aeruginosa Single- and Dual-Species Biofilms

Electrospun Nanofibrous Membranes Based on Citric Acid-Functionalized Chitosan Containing rGO-TEPA with Potential Application in Wound Dressings

Nanocomposite Material Synthesized Via Horizontal Vapor Phase Growth Technique: Evaluation and Application Perspective

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