Mechanism and color modulation of fungal bioluminescence

Kaskova, Zinaida M.; Dörr, Felipe Augusto; Petushkov, Valentin N.; Пуртов, К. В.; Tsarkova, Aleksandra S.; Родионова, Н. С.; Mineev, Konstantin S.; Guglya, Elena B.; Kotlobay, Alexey A.; Балеева, Надежда С.; Баранов, Михаил С.; Arseniev, Alexander S.; Gitelson, J. I.; Lukyanov, Sergey; Suzuki, Yuichi; Kanie, Shusei; Pinto, Ernani; Mascio, Paolo Di; Waldenmaier, Hans Eugene; Pereira, Tatiana Aparecida; Carvalho, Rodrigo Pimenta; Oliveira, Adriana Gonçalves; Oba, Yuichi; Bastos, E. L.; Stevani, Cassius V.; Yampolsky, Ilia V.

doi:10.1126/sciadv.1602847

Cited by 86 publications

(82 citation statements)

References 43 publications

(57 reference statements)

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“…This pathway can be nuclear encoded, as it is derived from a eukaryotic fungus, overcoming the challenges associated with plastid transformation. Additionally, this luciferase produces light in the green spectrum, which minimizes absorption by the predominant plant pigment, chlorophyll, and makes it spectrally separable from the other major luciferase-based reporters 13 .…”

Section: Introductionmentioning

confidence: 99%

Building customizable auto-luminescent luciferase-based reporters in plants

Khakhar

Starker

Chamness

et al. 2019

Preprint

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Bioluminescence is a powerful biological signal that scientists have repurposed to design reporters for gene expression in plants and animals. However, there are some downsides associated with the need to provide a substrate to these reporters, such as its high cost and non-uniform tissue penetration. In this work we reconstitute a fungal bioluminescence pathway (FBP) in planta using an easily composable toolbox of parts. We demonstrate that the FBP can create luminescence across various tissues in a broad range of plants without external substrate addition. We also show how our toolbox can be used to deploy the FBP in planta to build auto-luminescent reporters for the study of gene-expression and hormone fluxes. A low-cost imaging platform for gene expression profiling is also described. These experiments lay the groundwork for the future construction of programmable auto-luminescent plant traits, such as creating light driven plant-pollinator interactions or light emitting plant-based sensors.

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Section: Introductionmentioning

confidence: 99%

Building customizable auto-luminescent luciferase-based reporters in plants

Khakhar

Starker

Chamness

et al. 2019

Preprint

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“…Chemiluminescence consists on the emission of light resulting from a chemical reaction . Bioluminescence can be considered as a subtype of chemiluminescence, as it consists on light emission generated by an enzyme‐catalyzed reaction in living organisms, and it can be found in fireflies, fishes, fungi, earthworms, bacteria, and so on . Chemiluminescence is a more general process and can happen with or without a catalyzer.…”

Section: Introductionmentioning

confidence: 99%

Mechanistic insights into the efficient intramolecular chemiexcitation of dioxetanones from TD‐DFT and multireference calculations

Silva

Magalhães

2018

Int J of Quantum Chemistry

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Despite decades of research, it is still not clear what is the mechanism behind the efficient chemiexcitation of dioxetanones in chemiluminescent and bioluminescent reactions. In fact, long‐standing theories (charge transfer‐initiated luminescence and chemically induced electron‐exchange luminescence) have been demonstrated to not be able to explain this phenomenon. Herein, a theoretical approach using reliable and up‐to‐date methodology was used to address this problem, by focusing on model dioxetanones. Time‐dependent (TD)‐Density functional theory (DFT) and multireference complete‐active‐space second‐order perturbation theory (CASPT2) calculations provided evidence that points to efficient intramolecular chemiexcitation being the result of the reacting molecules having access to a long zone of the Potential energy surface (PES), within the biradicalar region, where S0 and S1 are degenerate. Molecules with inefficient chemiexcitation are unable to reach this zone of degeneracy. Our main finding is that access to the region of degeneracy appears to be given due to increased interaction between the keto and CO2 moieties, as supported by the use of the activation strain model and Born‐Oppenheimer molecular dynamics, which extends the biradical region by delaying the rupture of the peroxide ring. Increased interaction derives from attractive electrostatic interactions between the moieties of dioxetanone. Thus, we hypothesize that efficient chemiexcitation results not only from electron/charge transfer and subsequent charge annihilation but is instead based on the degree of interaction between the keto and CO2 moieties, which controls the access to a region of degeneracy between the ground and excited states.

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“…Very fascinatingly, these mushrooms such as Neonothopanus gardneri from Brazil's palm forests glow softly green in the dark (see Figure 4, bottom), mainly due to the bright "Hispidinbased" biofluorescence/luminescence: https://www.sciencenews.org/blog/science-ticker/ how-mushroom-gets-its-glow According to an international team led by Prof. Cassius Stevani in Brazil, this fluorescence (LED) depends on a unique ATP-independent oxidase/decarboxylase called "luciferase" and its substrate (luciferin) called "3-hydroxyl hispidin" (3-OH hispidin) [30]. Finally, after sunset, the oxidized form of 3-OH hispidin glows.…”

Section: Hispidin and Dkmentioning

confidence: 99%

Natural or Synthetic Anti-Melanogenic Compounds That Block the PDGFR-EGFR-PAK1-MITF-Tyrosinase Signaling Pathway

Miyata¹,

Pham-Thi²,

Ahn³

2017

J Dermatol Res Ther

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A natural sleeping pill and "elixir" (longevity-promoter) called "Melatonin" is one of the first natural anti-melanogenic compounds, and originally derived from bovine pineal glands. However, currently the majority of melatonin product in the market is chemically synthesized. Since then a wide variety of anti-melanogenic compounds such as curcumin, CAPE (Caffeic Acid Phenethyl Ester), and Artepillin C (ARC) were identified in nature as well as chemically synthesized compounds such as Gleevec. These anti-melanogenic compounds share a series of interesting biological properties such as anti-cancer, anti-inflammatory, anti-ageing (longevity-promoting), immune-boosting, anti-malaria, and anti-diabetic activities. However, the precise molecular mechanism underlying their anti-melanogenic action has remained to be clarified until recently. Finally we found the first clue to understanding of the "melanogenic" signalling pathway involving the major oncogenic/ageing kinase called PAK1 (RAC/CDC42-activated kinase 1) By siRNA-based silencing of PAK1 gene in murine melanoma cell line (B16F10): (i) The major growth hormone in serum called PDGF (Platelet-Derived Growth Factor) is essential for the inducible melanogenesis, but not for the exponential growth per se, and (ii) This cytokine activates its receptor called PDGFR, a Tyr-kinase on the cell surface, and induces another cell surface receptor Tyr-kinase called EGFR (Epidermal Growth Factor Receptor), to form a heterodimer with PDGFR, activating the down-stream melanogenic/oncogenic signalling pathway involving PAK1 and eventually MITF (Microphthalmia-Associated Transcription Factor) essential for expression of Tyrosinase and its Related Protein (TRP) genes. Melatonin and many other anti-melanogenic compounds block PAK1 directly or indirectly, and eventually down-regulate tyrosinase or TRP which is essential for melanin biosynthesis from an amino acid called tyrosine.

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Mechanism and color modulation of fungal bioluminescence

Abstract: Study of fungal bioluminescence mechanisms generates development of a multicolor enzymatic chemiluminescence system.

Cited by 86 publications

References 43 publications

Building customizable auto-luminescent luciferase-based reporters in plants

Building customizable auto-luminescent luciferase-based reporters in plants

Mechanistic insights into the efficient intramolecular chemiexcitation of dioxetanones from TD‐DFT and multireference calculations

Natural or Synthetic Anti-Melanogenic Compounds That Block the PDGFR-EGFR-PAK1-MITF-Tyrosinase Signaling Pathway

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