Mechanical and biological properties of keratose biomaterials

Guzman, Roche C. de; Merrill, Michelle R.; Richter, Jillian R.; Hamzi, Rawad I.; Greengauz-Roberts, Olga K.; Dyke, Mark Van

doi:10.1016/j.biomaterials.2011.07.054

Cited by 140 publications

(151 citation statements)

References 44 publications

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Section: Introductionmentioning

confidence: 99%

“…[19][20][21][22][23][24][25][26][27][28] KER possesses amino acid sequences similar to those found on an extracellular matrix (ECM), and because ECM is known to interact with integrins, which enable it to support cellular attachment, proliferation, and migration, KER-based materials are expected to have such properties as well. [19][20][21][22][23][24][25][26][27][28] Furthermore, KER is known to possess advantages for wound care, tissue reconstruction, cell seeding and diffusion, and drug delivery. [11][12][13][14][15][16][17][18][19][20] Unfortunately, in spite of its unique properties, KER has relatively poor mechanical properties, and as a consequence, it was not possible to fully exploit the unique properties of KER for various applications.…”

Section: Introductionmentioning

confidence: 99%

“…[11][12][13][14][15][16][17][18][19][20] Unfortunately, in spite of its unique properties, KER has relatively poor mechanical properties, and as a consequence, it was not possible to fully exploit the unique properties of KER for various applications. [19][20][21][22][23][24][25][26][27][28] To increase the structural strength of KER-based materials, attempts have been made to cross-link KER chains with a cross-linking agent or introduce functional groups to its amino acid residues via chemical reaction(s). [19][20][21][22][23][24][25][26][27][28] The rather complicated, costly, and multistep process is not desirable because it may inadvertently alter its unique properties, making the KER-based materials less biocompatible and toxic and removing or lessening its unique properties.…”

Section: Introductionmentioning

confidence: 99%

“…[19][20][21][22][23][24][25][26][27][28] To increase the structural strength of KER-based materials, attempts have been made to cross-link KER chains with a cross-linking agent or introduce functional groups to its amino acid residues via chemical reaction(s). [19][20][21][22][23][24][25][26][27][28] The rather complicated, costly, and multistep process is not desirable because it may inadvertently alter its unique properties, making the KER-based materials less biocompatible and toxic and removing or lessening its unique properties. A new method that can improve the structural strength of KER-based products not by synthetic methods but rather by the use of naturally occurring polysaccharides such as CEL is particularly needed.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

One-Pot Synthesis of Biocompatible Silver Nanoparticle Composites from Cellulose and Keratin: Characterization and Antimicrobial Activity

Tran

Prosenc

Franko

et al. 2016

ACS Appl. Mater. Interfaces

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A novel, simple method was developed to synthesize biocompatible composites containing 50% cellulose (CEL) and 50% keratin (KER) and silver in the form of either ionic (Ag + ) or Ag 0 nanoparticles (Ag + NPs or Ag 0 NPs). In this method, butylmethylimmidazolium chloride ([BMIm + Cl -]), a simple ionic liquid, was used as the sole solvent and silver chloride was added to the [BMIm + Cl -] solution of [CEL+KER] during the dissolution process. The silver in the composites can be maintained as ionic silver (Ag + ) or completely converted to metallic silver (Ag 0 ) by reducing it with NaBH4. The results of spectroscopy [Fourier transform infrared and X-ray diffraction (XRD)] and imaging [scanning electron microscopy (SEM)] measurements confirm that CEL and KER remain chemically intact and homogeneously distributed in the composites. Powder XRD and SEM results show that the silver in the [CEL+KER+Ag + ] and [CEL+KER+Ag 0 ] composites is homogeneously distributed throughout the composites in either Ag + (in the form of AgClNPs) or Ag 0 NPs form with sizes of 27 ± 2 or 9 ± 1 nm, respectively. Both composites were found to exhibit excellent antibacterial activity against many bacteria including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, methicillin-resistant S. aureus (MRSA), and vancomycin-resistant Enterococus faecalis (VRE). The antibacterial activity of both composites increases with the Ag + or Ag 0 content in the composites. More importantly, for the same bacteria and the same silver content, the [CEL+KER+AgClNPs] composite is relatively more toxic than [CEL+KER+Ag 0 NPs] composite. Experimental results confirm that there was hardly any Ag 0 NPs release from the [CEL+KER+Ag 0 NPs] composite, and hence its antimicrobial activity and NOT THE PUBLISHED VERSION; this is the author's final, peer-reviewed manuscript. The published version may be accessed by following the link in the citation at the bottom of the page. Vol 8, No. 50 (2016): pg. 34791-34801. DOI. This article is © American Chemical Society and permission has been granted for this version to appear in e-Publications@Marquette. American Chemical Society does not grant permission for this article to be further copied/distributed or hosted elsewhere without the express permission from American Chemical Society. ACS Applied materials & Interfaces,3 biocompatibility is due not to any released Ag 0 NPs but rather entirely to the Ag 0 NPs embedded in the composite. Both AgClNPs and Ag 0 NPs were found to be toxic to human fibroblasts at higher concentration (>0.72 mmol), and for the same silver content, the [CEL+KER+AgClNPs] composite is relatively more toxic than the [CEL+KER+Ag 0 NPs] composite. As expected, by lowering the Ag 0 NPs concentration to 0.48 mmol or less, the [CEL+KER+Ag 0 NPs] composite can be made biocompatible while still retaining its antimicrobial activity against bacteria such as E. coli, S. aureus, P. aeruginosa, MRSA, and VRE. These results, together with our previous finding that [CEL+KER] composites can ...

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

One-Pot Synthesis of Biocompatible Silver Nanoparticle Composites from Cellulose and Keratin: Characterization and Antimicrobial Activity

Tran

Prosenc

Franko

et al. 2016

ACS Appl. Mater. Interfaces

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“…18,22,25 Compared to materials that release drugs simply via diffusion and are known to display a short burst of antibiotic release, keratose hydrogels have been shown to support the sustained release of the antibiotic ciprofloxacin for > 1 week in vitro. 23 Further, keratose hydrogels loaded with 2 mg/mL ciprofloxacin inhibit S. aureus growth for 23 days in vitro.…”

Section: Introductionmentioning

confidence: 99%

Ciprofloxacin-Loaded Keratin Hydrogels PreventPseudomonas aeruginosaInfection and Support Healing in a Porcine Full-Thickness Excisional Wound

Roy

Tomblyn

Burmeister

et al. 2015

Advances in Wound Care

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Objective: Cutaneous wound infection can lead to impaired healing, multiple surgical procedures, and increased hospitalization time. We tested the effectiveness of keratin-based hydrogels (termed ''keratose'') loaded with ciprofloxacin to inhibit infection and support healing when topically administered to porcine excision wounds infected with Pseudomonas aeruginosa. Approach: Using a porcine excisional wound model, 10 mm full-thickness wounds were inoculated with 10 6 colony-forming units of P. aeruginosa and treated on days 1 and 3 postinoculation with ciprofloxacin-loaded keratose hydrogels. Bacteria enumeration and wound healing were assessed on days 3, 7, and 11 postinjury. Results: Ciprofloxacin-loaded keratose hydrogels reduced the amount of P. aeruginosa in the wound bed by 99.9% compared with untreated wounds on days 3, 7, and 11 postinjury. Ciprofloxacin-loaded keratose hydrogels displayed decreased wound contraction and reepithelialization at day 7 postinjury. By day 11, wounds treated with ciprofloxacin-keratose hydrogels contained collagen-rich granulation tissue and myofibroblasts. Wounds treated with ciprofloxacin-loaded keratose hydrogels exhibited a transient increase in macrophages in the wound bed at day 7 postinjury that subsided by day 11. Innovation: Current therapies for wound infection include systemic antibiotics, which could lead to antibiotic resistance, and topical antimicrobial treatments, which require multiple applications and can delay healing. Here, we show that ciprofloxacin-loaded keratose hydrogels inhibit cutaneous wound infection without interfering with key aspects of the healing process including granulation tissue deposition and remodeling. Conclusions: Ciprofloxacin-loaded keratose hydrogels have the potential to serve as a point-of-injury antibiotic therapy that prevents infection and supports healing following cutaneous injury.

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Keratin

Dyke

2016

Biomaterials From Nature for Advanced Devices and Therapies

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Mechanical and biological properties of keratose biomaterials

Cited by 140 publications

References 44 publications

One-Pot Synthesis of Biocompatible Silver Nanoparticle Composites from Cellulose and Keratin: Characterization and Antimicrobial Activity

One-Pot Synthesis of Biocompatible Silver Nanoparticle Composites from Cellulose and Keratin: Characterization and Antimicrobial Activity

Ciprofloxacin-Loaded Keratin Hydrogels PreventPseudomonas aeruginosaInfection and Support Healing in a Porcine Full-Thickness Excisional Wound

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