Measuring the effects of postmortem time and age on mouse lens elasticity using atomic force microscopy

Batchelor, Wyndham More; Heilman, Bianca Maceo; Arrieta-Quintero, E.; Ruggeri, Marco; Parel, Jean-Marie A.; Manns, Fabrice; Cabrera-Ghayouri, Sara; Dibas, Mohammed; Ziebarth, Noël Marysa

doi:10.1016/j.exer.2021.108768

Cited by 2 publications

(1 citation statement)

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“…Using AFM, the Young's modulus, a mechanical property that is indicative of tissue stiffness, can be determined quantitatively [30]. In the eye, AFM has been successfully used to evaluate stiffness across various ocular tissues and in a broad range of mammals, including corneal stiffness in mice [31] and humans [32,33]; trabecular meshwork stiffness in rats [34]; lens stiffness in mice [35] and non-human primates [36]; and the sclera and optic nerve head stiffness in pigs [37,38], mice [39], rats [40], and humans [41].…”

Section: Of 12mentioning

confidence: 99%

Collagen Mimetic Peptides Promote Repair of MMP-1-Damaged Collagen in the Rodent Sclera and Optic Nerve Head

Bou Ghanem,

Koktysh,

Baratta

et al. 2023

IJMS

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The structural and biomechanical properties of collagen-rich ocular tissues, such as the sclera, are integral to ocular function. The degradation of collagen in such tissues is associated with debilitating ophthalmic diseases such as glaucoma and myopia, which often lead to visual impairment. Collagen mimetic peptides (CMPs) have emerged as an effective treatment to repair damaged collagen in tissues of the optic projection, such as the retina and optic nerve. In this study, we used atomic force microscopy (AFM) to assess the potential of CMPs in restoring tissue stiffness in the optic nerve head (ONH), including the peripapillary sclera (PPS) and the glial lamina. Using rat ONH tissue sections, we induced collagen damage with MMP-1, followed by treatment with CMP-3 or vehicle. MMP-1 significantly reduced the Young’s modulus of both the PPS and the glial lamina, indicating tissue softening. Subsequent CMP-3 treatment partially restored tissue stiffness in both the PPS and the glial lamina. Immunohistochemical analyses revealed reduced collagen fragmentation after MMP-1 digestion in CMP-3-treated tissues compared to vehicle controls. In summary, these results demonstrate the potential of CMPs to restore collagen stiffness and structure in ONH tissues following enzymatic damage. CMPs may offer a promising therapeutic avenue for preserving vision in ocular disorders involving collagen remodeling and degradation.

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