2017
DOI: 10.21769/bioprotoc.2086
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Abstract: Oxidative stress has been proposed to be one of the main causes of aging and has been implicated in the pathogenesis of many diseases. Sensitivity to oxidative stress can be measured by quantifying survival following exposure to a reactive oxygen species (ROS)-generating compound such as paraquat or juglone. Sensitivity to oxidative stress is a balance between basal levels of ROS, the ability to detoxify ROS, and the ability to repair ROS-mediated damage.

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Cited by 46 publications
(16 citation statements)
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“…We utilized four different stresses: oxidative stress through exposure to 2 mM paraquat for 24 hours, heat stress through exposure to 37°C heat for 2 hours, osmotic stress through exposure to 400 mM NaCl for 24 hours, and complete anoxia for 24 hours. These specific stress paradigms have been previously utilized by our laboratory as well as several others 38‐44 . In this experiment, we chose to expose worms to a shorter duration or smaller dose of stress than we would use in a test of stress resistance to ensure that all worms would survive, and so that we could observe the effect of stress, rather than dying, on mitochondrial morphology.…”
Section: Resultsmentioning
confidence: 99%
“…We utilized four different stresses: oxidative stress through exposure to 2 mM paraquat for 24 hours, heat stress through exposure to 37°C heat for 2 hours, osmotic stress through exposure to 400 mM NaCl for 24 hours, and complete anoxia for 24 hours. These specific stress paradigms have been previously utilized by our laboratory as well as several others 38‐44 . In this experiment, we chose to expose worms to a shorter duration or smaller dose of stress than we would use in a test of stress resistance to ensure that all worms would survive, and so that we could observe the effect of stress, rather than dying, on mitochondrial morphology.…”
Section: Resultsmentioning
confidence: 99%
“…It has been suggested that microbial infections induce oxidative stress in C. elegans (Bolm et al, 2004;Miller et al, 2015;Sem and Rhen, 2012). We therefore treated worms with paraquat that is commonly used as an oxidative stress inducer (Senchuk et al, 2017). Fig.…”
Section: Endotube Dysfunction Correlates With Impaired Oxidative and mentioning
confidence: 99%
“…Methyl viologen dichloride hydrate ( paraquat; Sigma-Aldrich) was used for oxidative stress assays at a final concentration of 200 mM in NGM agar (Senchuk et al, 2017). Poured plates were stored overnight at room temperature following inoculation with 50 µl 10× concentrated overnight culture of OP50.…”
Section: Stress Assaysmentioning
confidence: 99%
“…[5]. In addition, oxidative stress has been proposed to be one of the major causes of aging and has been implicated in the pathogenesis of many diseases [8]. Therefore, a certain concentration of antioxidants will contribute to the longevity of organisms.…”
Section: Introductionmentioning
confidence: 99%