Measurements of Aqueous Humor Turnover Rates Using a Gamma Probe

Dynamics of Ascorbate in the Aqueous Humor and Tissues Surrounding Ocular Chambers

“…In some experiments, the perfusate contained plasma level (0.04-0.06 mXt) ascorbate, and thiourea was added to the perfusate in order to minimize oxida tion of ascorbate [5]. The aqueous humor turnover rate (K out) in the enucleated eye was determined by the decay of l4C-inulin injected into the anterior chamber [6]. After I h of perfusion, both the anterior and poste rior aqueous humor and the vitreous humor were with drawn and mixed with ice-cold 5% metaphosphoric acid.…”

Dynamics of Ascorbate in the Aqueous Humor and Tissues Surrounding Ocular Chambers

“…In some experiments, the perfusate contained plasma level (0.04-0.06 mXt) ascorbate, and thiourea was added to the perfusate in order to minimize oxida tion of ascorbate [5]. The aqueous humor turnover rate (K out) in the enucleated eye was determined by the decay of l4C-inulin injected into the anterior chamber [6]. After I h of perfusion, both the anterior and poste rior aqueous humor and the vitreous humor were with drawn and mixed with ice-cold 5% metaphosphoric acid.…”

Pharmacological Study on the Effects of Some Ocular Hypotensive Drugs on Aqueous Humor Formation in the Arterially Perfused Enucleated Rabbit Eye

“…The aqueous humor turnover rate (k out) was de termined from the decay of intracamcrally adminis tered '"TRISA [Macri and O'Rourke, 1970]. Approxi mately 10 pi of li5I-RISA which had radioactivity of 20,000-40,000 cpm was introduced into the anterior chamber of the enucleated eye of New Zealand white rabbits (2.5-3 kg).…”

“…Approxi mately 10 pi of li5I-RISA which had radioactivity of 20,000-40,000 cpm was introduced into the anterior chamber of the enucleated eye of New Zealand white rabbits (2.5-3 kg). The radioactivity was continuously monitored by a gamma probe and recorded at 5-min intervals [Macri and O'Rourke, 1970], In brief, the cannulated eyes were placed in a position with the cornea facing upwards. A gamma probe with a diameter of 12 mm, which covered the total area of the anterior chamber, was fixed close to the cornea at a distance of approximately 5 mm.…”

The Pressure Dependency of Aqueous Humor Formation in the Anesthetized Cat

“…The rate of AH turnover (K0UI) was measured by following the decay of intracamerally injected (one bolus) 125I-tagged human serum albumin (RISA), using a gamma probe [3,6], The AH was continuously stirred [1,3] during the turnover study to ensure uniform distribu tion of the isotope. Calculations of AH production were made by multiplying the Kout m in"1 of the radioactive albumin by a presumed anterior chamber volume of 900 pi.…”

Dynamics of Ascorbate in the Aqueous Humor and Tissues Surrounding Ocular Chambers

“…In some experiments, the perfusate contained plasma level (0.04-0.06 mXt) ascorbate, and thiourea was added to the perfusate in order to minimize oxida tion of ascorbate [5]. The aqueous humor turnover rate (K out) in the enucleated eye was determined by the decay of l4C-inulin injected into the anterior chamber [6]. After I h of perfusion, both the anterior and poste rior aqueous humor and the vitreous humor were with drawn and mixed with ice-cold 5% metaphosphoric acid.…”

Pharmacological Study on the Effects of Some Ocular Hypotensive Drugs on Aqueous Humor Formation in the Arterially Perfused Enucleated Rabbit Eye

“…The aqueous humor turnover rate (k out) was de termined from the decay of intracamcrally adminis tered '"TRISA [Macri and O'Rourke, 1970]. Approxi mately 10 pi of li5I-RISA which had radioactivity of 20,000-40,000 cpm was introduced into the anterior chamber of the enucleated eye of New Zealand white rabbits (2.5-3 kg).…”

“…Approxi mately 10 pi of li5I-RISA which had radioactivity of 20,000-40,000 cpm was introduced into the anterior chamber of the enucleated eye of New Zealand white rabbits (2.5-3 kg). The radioactivity was continuously monitored by a gamma probe and recorded at 5-min intervals [Macri and O'Rourke, 1970], In brief, the cannulated eyes were placed in a position with the cornea facing upwards. A gamma probe with a diameter of 12 mm, which covered the total area of the anterior chamber, was fixed close to the cornea at a distance of approximately 5 mm.…”

The Pressure Dependency of Aqueous Humor Formation in the Anesthetized Cat

“…The rate of AH turnover (K0UI) was measured by following the decay of intracamerally injected (one bolus) 125I-tagged human serum albumin (RISA), using a gamma probe [3,6], The AH was continuously stirred [1,3] during the turnover study to ensure uniform distribu tion of the isotope. Calculations of AH production were made by multiplying the Kout m in"1 of the radioactive albumin by a presumed anterior chamber volume of 900 pi.…”

Dynamics of Ascorbate in the Aqueous Humor and Tissues Surrounding Ocular Chambers

“…In some experiments, the perfusate contained plasma level (0.04-0.06 mXt) ascorbate, and thiourea was added to the perfusate in order to minimize oxida tion of ascorbate [5]. The aqueous humor turnover rate (K out) in the enucleated eye was determined by the decay of l4C-inulin injected into the anterior chamber [6]. After I h of perfusion, both the anterior and poste rior aqueous humor and the vitreous humor were with drawn and mixed with ice-cold 5% metaphosphoric acid.…”

Pharmacological Study on the Effects of Some Ocular Hypotensive Drugs on Aqueous Humor Formation in the Arterially Perfused Enucleated Rabbit Eye

“…The aqueous humor turnover rate (k out) was de termined from the decay of intracamcrally adminis tered '"TRISA [Macri and O'Rourke, 1970]. Approxi mately 10 pi of li5I-RISA which had radioactivity of 20,000-40,000 cpm was introduced into the anterior chamber of the enucleated eye of New Zealand white rabbits (2.5-3 kg).…”

“…Approxi mately 10 pi of li5I-RISA which had radioactivity of 20,000-40,000 cpm was introduced into the anterior chamber of the enucleated eye of New Zealand white rabbits (2.5-3 kg). The radioactivity was continuously monitored by a gamma probe and recorded at 5-min intervals [Macri and O'Rourke, 1970], In brief, the cannulated eyes were placed in a position with the cornea facing upwards. A gamma probe with a diameter of 12 mm, which covered the total area of the anterior chamber, was fixed close to the cornea at a distance of approximately 5 mm.…”

The Pressure Dependency of Aqueous Humor Formation in the Anesthetized Cat

“…The rate of AH turnover (K0UI) was measured by following the decay of intracamerally injected (one bolus) 125I-tagged human serum albumin (RISA), using a gamma probe [3,6], The AH was continuously stirred [1,3] during the turnover study to ensure uniform distribu tion of the isotope. Calculations of AH production were made by multiplying the Kout m in"1 of the radioactive albumin by a presumed anterior chamber volume of 900 pi.…”