Journal of Chromatography A 2012 DOI: 10.1016/j.chroma.2011.12.055 View full text
Fabrice Gritti, Georges Guiochon

Abstract: We investigated the mass transfer mechanism in four research prototypes of silica monolithic columns of the second generation provided by their manufacturer (Merck KGaA, Darmstadt, Germany). The heights equivalent to a theoretical plate (HETP) of these columns were measured. The different contributions to the total HETP (longitudinal diffusion term B/u(S), skeleton/eluent mass transfer resistance term Cu(S), and eddy diffusion term A) were determined experimentally for a non-retained (uracil) and for a retaine…

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