Measurement of myelin basic protein in the cerebrospinal fluid of dogs with degenerative myelopathy

Oji, Takashi; Kamishina, Hiroaki; Cheeseman, Jennifer A.; Clemmons, Roger M.

doi:10.1111/j.1939-165x.2007.tb00225.x

Cited by 16 publications

(14 citation statements)

References 16 publications

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“…This assay is intended for the measurement of human MBP in CSF, and contains a goat antihuman MBP antibody. Previously published data 18 have supported the detection of canine MBP by the goat antihuman MBP antibody. In-house validation of the assay in the study reported here consisted of intra-and interassay variability, dilutional parallelism, and spiking recovery.…”

Section: Sample Collection and Testingmentioning

confidence: 67%

“…30 Dogs with degenerative myelopathy and control dogs did not have significant differences in cisternal CSF MBP concentration, although dogs with degenerative myelopathy did have higher CSF MBP concentration compared with controls when samples from the lumbar cistern were analyzed. 18 Sample size and the site of CSF collection may have contributed to the lack of statistical significance for some comparisons. Differences in breed distribution and chondrodystrophoid status between the control and affected populations also may have influenced measured associations.…”

Section: Discussionmentioning

confidence: 99%

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Cerebrospinal Fluid Myelin Basic Protein as a Prognostic Biomarker in Dogs with Thoracolumbar Intervertebral Disk Herniation

Levine¹,

Levine²,

Witsberger³

et al. 2010

Journal of Veterinary Internal Medicine

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Background: Release of myelin basic protein (MBP) into the cerebrospinal fluid (CSF) is associated with active demyelination and correlates with outcome in various neurological diseases.Hypothesis/Objectives: To describe associations among CSF MBP concentration, initial neurological dysfunction, and long-term ambulatory outcome in dogs with acute thoracolumbar intervertebral disk herniation (IVDH).Animals: Five hundred and seventy-four dogs with acute thoracolumbar IVDH and 16 clinically normal dogs. Methods: Prospective case series clinical study. Signalment, initial neurological dysfunction as determined by a modified Frankel score (MFS), and ambulatory outcome at 43-month follow-up were recorded. Cisternal CSF MBP concentration was determined by an ELISA. Associations were estimated between CSF MBP concentration and various clinical parameters.Results: Dogs with thoracolumbar IVDH that did not ambulate at follow-up had a higher CSF MBP concentration (median, 3.56 ng/mL; range, 0.59-51.2 ng/mL) compared with control dogs (median, 2.22 ng/mL; range, 0-3.82 ng/mL) (P 5 .032). A CSF MBP concentration of !3 ng/mL had a sensitivity of 78% and specificity of 76% to predict an unsuccessful outcome based on receiver-operating characteristics curve analysis (area under the curve 50.688, P 5 .079). Affected dogs with a CSF MBP concentration !3 ng/mL had 0.09 times the odds of ambulation at follow-up compared with affected dogs with CSF MBP concentration o3 ng/mL when adjusted for initial MFS (95% confidence interval 0.01-0.66, P 5 .018).Conclusions and Clinical Importance: These results would suggest that CSF MBP concentration may be useful as an independent prognostic indicator in dogs with thoracolumbar IVDH.

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Section: Sample Collection and Testingmentioning

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Cerebrospinal Fluid Myelin Basic Protein as a Prognostic Biomarker in Dogs with Thoracolumbar Intervertebral Disk Herniation

Levine¹,

Levine²,

Witsberger³

et al. 2010

Journal of Veterinary Internal Medicine

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“…Therefore, pNF can be readily detected in the serum and CSF (65). A recent study also examined this neurofilament in dogs with spinal cold injury (SCI) following intervertebral disc disease (43). The authors found that mean pNF-H serum levels were significantly higher in dogs with no pain perception than in those with intact pain perception.…”

Section: Introductionmentioning

confidence: 96%

“…However, some dogs with SCI did not have high pNF-H levels between day one and day three after injury. Furthermore, dogs with no pain perception, which did not regain ambulation following surgery, had significantly higher pNF-H levels in comparison to those that regained ambulation following surgery (43). Hayakawa et al (25) found the plasma pNF-H levels to be detectable within 96 h in humans following SCI and to remain elevated for up to 21 days.…”

Section: Introductionmentioning

confidence: 99%

Biomarkers of injury to neural tissue in veterinary medicine

Płonek

Wrzosek

Nicpoń

2016

Journal of Veterinary Research

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There are numerous biomarkers of central and peripheral nervous system damage described in human and veterinary medicine. Many of these are already used as tools in the diagnosis of human neurological disorders, and many are investigated in regard to their use in small and large animal veterinary medicine. The following review presents the current knowledge about the application of cell-type (glial fibrillary acidic protein, neurofilament subunit NF-H, myelin basic protein) and central nervous system specific proteins (S100B, neuron specific enolase, tau protein, alpha II spectrin, ubiquitin carboxy-terminal hydrolase L1, creatine kinase BB) present in the cerebrospinal fluid and/or serum of animals in the diagnosis of central or peripheral nervous system damage in veterinary medicine.

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“…Other protein types that have been investigated for their diagnostic potential include myelin basic protein, associated with degenerative myelopathy (Oji et al, 2007), and C-reactive protein (CRP) which may help distinguishing bacterial and viral meningoencephalitis (Fishman, 1992).…”

Section: Total Protein Concentrationmentioning

confidence: 99%

Cerebrospinal fluid analysis

Polizopoulou

2017

J Hellenic Vet Med Soc

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The significance of cerebrospinal fluid (CSF) analysis has been compared to that of complete blood counts for systemic diseases. CSF changes generally follow specific patterns depending on the etiopathogenesis (inflammatory, metabolic, neoplastic) of neurological diseases, thus indicating which further diagnostic modalities should be applied. CSF collection requires operator skills and knowledge of sampling indications and contraindications. Analysis includes physical examination (colour, clarity, viscosity), cytological and biochemical analysis, microbiological and immunologic testing. Despite its limitations, CSF analysis is a useful diagnostic tool, in particular when results are interpreted in combination with historical information, clinical examination and the findings of clinicopathological and diagnostic imaging testing.Keywords: cerebrospinal fluid, diagnosis, neurological diseases, animals ΠΕΡΙΛΗΨΗ Η ανάλυση του εγκεφαλονωτιαίου υγρού (ΕΝΥ) είναι η σημαντικότερη εργαστηριακή εξέταση στη διαγνωστική διερεύνηση των παθήσεων του νευρικού συστήματος των ζώων, που ανάλογα με την αιτιολογία τους προκαλούν συνήθως συγκεκριμένου τύπου παθολογικές μεταβολές στη σύστασή του. Η δειγματοληψία του ΕΝΥ γίνεται με ατλαντοϊνιακή ή οσφυϊκή παρακέντηση του υπαραχνοειδούς χώρου, με την προϋπόθεση ότι δεν υπάρχει οποιαδήποτε από τις γνωστές αντενδείξεις (αύξηση ενδοκρανια-κής πίεσης, αδυναμία χορήγησης γενικής αναισθησίας, απειρία εξεταστή). Η ανάλυση περιλαμβάνει την εκτίμηση των φυσικών χαρακτηριστικών (χρώμα, διαφάνεια, ιξώδες), την κυτταρολογική (ποσοτική και ποιοτική), τη βιοχημική (μέτρηση ολικών πρω-τεϊνών και γλυκόζης), τη μικροβιολογική και τις ανοσολογικές εξετάσεις. Η διαγνωστική αξία των ευρημάτων της ανάλυσης του ΕΝΥ ενισχύεται περαιτέρω αν συνδυαστεί με τα στοιχεία του ιστορικού, της κλινικής και της νευρολογικής εξέτασης και ιδιαί-τερα με τα αποτελέσματα των ειδικών απεικονιστικών εξετάσεων.Λέξεις ευρετηρίασης: εγκεφαλονωτιαίο υγρό, διάγνωση, νοσήματα νευρικού συστήματος ζώων

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Measurement of myelin basic protein in the cerebrospinal fluid of dogs with degenerative myelopathy

Abstract: The MBP ELISA has potential as a supplemental test of CSF to diagnose demyelinating disorders in dogs.

Cited by 16 publications

References 16 publications

Cerebrospinal Fluid Myelin Basic Protein as a Prognostic Biomarker in Dogs with Thoracolumbar Intervertebral Disk Herniation

Cerebrospinal Fluid Myelin Basic Protein as a Prognostic Biomarker in Dogs with Thoracolumbar Intervertebral Disk Herniation

Biomarkers of injury to neural tissue in veterinary medicine

Cerebrospinal fluid analysis

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