Measurement of gamma-aminobutyric acid in human cerebrospinal fluid: Radioreceptor assay using [3H]muscimol

Baraczka, Krisztina; Sperk, Günther

doi:10.1016/0009-8981(81)90139-x

Cited by 8 publications

(4 citation statements)

References 8 publications

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“…The mean concentrations of GABA that we have found in the CSF of control subjects (Table I ) are considerably lower than those reported by investigators who have used the radioreceptor assay (Enna et al, 1977u,h;Baraczka and Sperk, 1981) or gas chromatography-mass spectrometric techniques (Huizinga et al, 1978;Faull et al, 1978). We think it likely that both of these methods may yield artifactually high GABA values because of the presence of conjugated GABA compounds in CSF.…”

Section: Discussioncontrasting

confidence: 67%

“…Three analytical methods are currently consid-e r e d suitable f o r d e t e r m i n i n g t h e v e r y small amounts of GABA present in CSF. A radioreceptor assay, based on the displacement of [3H]GABA bound to specific synaptic receptors in rat brain tissue by GABA present in CSF, has been widely used (Enna and Snyder, 1976; E n n a e t al., 1977u,b; Baraczka and Sperk, 1981). A potential disadvantage o f this method is that homocarnosine ( yaminobutyrylhistidine), which is present in human CSE in concentrations at least 10 times greater than those of GABA, also binds t o GABA receptors in brain, albeit with only 2% of the affinity of GABA (Enna and Snyder, 1976).…”

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confidence: 99%

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Human CSF GABA Concentrations: Revised Dowd for Controls, but Not Decreased in Huntington's Chorea

Perry

Hansen

Wall

et al. 1982

Journal of Neurochemistry

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gamma-Aminobutyric acid (GABA) concentrations were measured in CSF specimens from two large groups of control subjects, one without neurological or psychiatric disease, and one with a variety of neurological disorders not known to involve altered GABAergic function in brain. CSF GABA was also measured in patients with Huntington's chorea and in patients with other choreiform disorders. GABA was measured in CSF by a modification of the ion exchange-fluorometric method that featured use of a relatively large cation exchange column, and a markedly decreased quantity of sulfosalicylic acid for deproteinization of CSF. Mean BABA concentrations in CSF were 87 and 77 nmol/liter for neurologically normal and abnormal control subjects, 82 nmol/liter for the Huntington's chorea patients, and 105 nmol/liter for patients with other forms of chorea. The mean concentration of homocarnosine was not reduced in CSF of Huntington's chorea patients as compared with controls. Mean CSF GABA concentrations found in control subjects were less than half the lowest control means previously reported. These low values are attributable in part to a reduction in on-column hydrolysis of conjugated forms of GABA in CSF, which can be produced by excessive sulfosalicylic acid, and in part to improved chromatographic resolution of GABA from other unknown o-phthalaldehyde-reactive compounds in CSF. Analysis of free GABA in CSF does not appear useful for diagnosis of suspected Huntington's chorea, nor as a possible predictive test for persons genetically at risk for Huntington's chorea.

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Section: Discussioncontrasting

confidence: 67%

mentioning

confidence: 99%

Human CSF GABA Concentrations: Revised Dowd for Controls, but Not Decreased in Huntington's Chorea

Perry

Hansen

Wall

et al. 1982

Journal of Neurochemistry

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“…Eine Bedeutung der 4-Aminobuttration im Gehirn durch direkte Gewebsentnahme tersäure als diagnostisches Kriterium bei verschiedeist zwar durchführbar, wegen des damit verbunde- (6,7,8). Nachteilig ist die verhältnismäßig geringe Spezifität.…”

Section: Einführungunclassified

Quantitative fluorimetrische Bestimmung der 4-Aminobuttersäure aus Liquor mit einem Aminosäurenanalysator

Uhlhaas¹,

Olek²

1984

Clinical Chemistry and Laboratory Medicine

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Zusammenfassung: Wir beschreiben eine schnelle, empfindliche und quantitative Bestimmungsmethode der 4-Aminobuttersäure aus menschlichem Liquor unter Verwendung eines modifizierten, handelsüblichen Aminosäurenanalysators mit Fluoreszenzdetektion.Insbesondere wurden die hydrolytischen Eigenschaften der 5-Sulfosalicylsäure, die wesentlichen Einfluß auf das Meßergebnis haben, im Liquor untersucht.Aus der gleichen Probenvorbereitungsmethode mit verschiedenen Mengen an Sulfosalicylsäure (50 g/l und 5 g/l) resultierten unterschiedliche Ergebnisse für 4-Aminobuttersäure (231,6 ± 46,6 und 90.0 ± 24,9 nmol/1).Eine Enteiweißung des Liquors durch Ultrafiltration ergab sehr viel niedrigere Meßwerte (57,6 ± 16,0 nmol/1). Durch eine Minimierung der Zeit zwischen Lumbaipunktion und Messen der Probe sowie Einsatz von Ultrafiltern konnten wir nachweisen, daß die nach Sulfosalicylsäureenteiweißung (5 g/l Liquor) gemessenen Kontrollwerte für 4-Aminobuttersäure nur unwesentlich über den wahren Werten liegen. Quantitative fluorometric measurement of 4-aminobutyric acid in human cerebrospinal fluid with an amino acid analyserSummary: A rapid and simple procedure is described for the determination of 4-aminobutyric acid in human CSF. The o-phüialdialdehyde derivative of 4-aminobutyric acid is analysed with a slightly modified commercially available Amino Acid Analyser.We investigated the sample preparation with special emphasis on the sulphosalicylic acid-induced hydrolysis of 4-aminobütyric acid containing compounds in human liquor. Our experiments demonstrate that the amount of estimated 4-aminöbutyf ic acid depends considerably on the sulphosalicylic acid concentration used for protein precipitation.

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“…Development of a new, sensitive, reproducible, and simple method to investigate GHB and its metabolites in tissues could help to determine PMI more precisely. However, both chromatographic techniques presented in the literature did not allow the simultaneous analysis of GHB, 1,4-BD, GBL, and GABA in animal or human tissues. − …”

mentioning

confidence: 99%

GC/MS Profiling of γ-Hydroxybutyrate and Precursors in Various Animal Tissues Using Automatic Solid-Phase Extraction. Preliminary Investigations of Its Potential Interest in Postmortem Interval Determination

Richard¹,

Ling²,

Authier³

et al. 2005

Anal. Chem.

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To quantify gamma-hydroxybutyrate (GHB) and its physiological metabolites, gamma-aminobutyric acid (GABA), 1,4-butanediol (1,4-BD), and gamma-butyrolactone (GBL) in various animal tissues (kidney, muscle, heart, liver, blood, brain cortex, thalamus, hypothalamus, hippocampus, or pons), an original gas chromatographic/mass spectrometric method with a automated solid-phase extraction by Oasis MCX cartridges on a Gilson Aspec Xli was developed. Using such apparatus allowed the limit of detection (LOD) of target compounds to be significantly lowered (LOD: 0.027, 0.025, and 5.7 microg/mL for GHB, 1,4-BD, and GABA, respectively, in 200 microL or microg of sample). After validation of each analytical step, the satisfactory performances of the apparatus in conjunction with the rapidity and ease of the extraction step make it suitable for simultaneous assay of GHB, 1,4-BD, GBL, and GABA. The method was used to test the correlation between GHB levels in tissues obtained at different times after death of male Sprague-Dawley rats and the postmortem interval. Preliminary results show a linear increase of GHB levels in relation to time of death in thoracic blood and central nervous system of animals kept at 15 and 20 degrees C.

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Measurement of gamma-aminobutyric acid in human cerebrospinal fluid: Radioreceptor assay using [3H]muscimol

Cited by 8 publications

References 8 publications

Human CSF GABA Concentrations: Revised Dowd for Controls, but Not Decreased in Huntington's Chorea

Human CSF GABA Concentrations: Revised Dowd for Controls, but Not Decreased in Huntington's Chorea

Quantitative fluorimetrische Bestimmung der 4-Aminobuttersäure aus Liquor mit einem Aminosäurenanalysator

GC/MS Profiling of γ-Hydroxybutyrate and Precursors in Various Animal Tissues Using Automatic Solid-Phase Extraction. Preliminary Investigations of Its Potential Interest in Postmortem Interval Determination

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