Measurement of Electron Transport System (ETS) activity in soil

Trevors, J. T.; Mayfield, C. I.; Inniss, W. E.

doi:10.1007/bf02010449

Cited by 151 publications

(58 citation statements)

References 6 publications

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“…INT reduction assays similar to the one used here have been suggested as a quantitative measure of metabolic activity in a variety of systems, including marine sediments (Houri-Davignon et al 1989), estuarine sediments (Trevors 1984a), freshwater sediments (Trevors 1984b), soils (Trevors et al 1982, Trevors 1984c, von Mersi and Schinner 1991, Friedel et al 1994, Garcia et al 1994, river biofilms (Blenkinsopp and Lock 1990), and peat (Gammelgaard et al 1992). In many of these studies, INT reduction was correlated with some independent measure of metabolic activity, most commonly 02 consumption or CO2 production.…”

Section: Discussionmentioning

confidence: 99%

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Tetrazolium reduction in acidicSphagnum-derived peat

et al. 1998

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Assessment of how carbon storage in peat and gas fluxes across the peatland,/atmosphere interface may be affected by predicted climate changes requires an understanding of the controls on C mineralization in peat. Toward this end, our goal was to develop the INT[2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride] reduction procedure for use in the field as a rapid and reliable approach to estimating C mineralization (CO2 and CH4 production) in peat under near-natural conditions with considerable spatial and temporal replication. Sphagnum peat samples collected from three sites were divided and analyzed for INT reduction activity, as well as for production of CO2 and CH4, under both oxic and anoxic conditions. For peat from each site, we found no significant relationships between INT reduction and oxic or anoxic CO2 or CH4 production. Although INT is reduced in acidic Sphagnum peat, INT reduction rates do not provide a usable index of C mineralization.

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Section: Discussionmentioning

confidence: 99%

“…Trevors et al (1982) and Houri-Davignon et al (1989) reported slopes of 0.0155 and 0.0377, respectively. Despite significant two-way interactions in the ANOVA (Table 1), an a posteriori comparison of all means revealed treatment effects (Figure 1).…”

Section: Formalin Int and Extractant Effects On Int Reduction Measumentioning

confidence: 99%

Tetrazolium reduction in acidicSphagnum-derived peat

et al. 1998

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“…Arylsulfatase enzymes catalyze the hydrolysis of sulfate esters resulting in the release of SO 4 2− , being important to S cycling processes in wetland soils (Tabatabai and Bremner 1970). Finally, dehydrogenase enzyme activity is used as a measurement of the overall biological activity in soils (Trevors et al 1982).…”

Section: Extracellular Enzyme Activities and Nutrient Availability Inmentioning

confidence: 99%

Biogeochemical Indicators of Nutrient Enrichments in Wetlands: The Microbial Response as a Sensitive Indicator of Wetland Eutrophication

Sánchez-Carrillo

Reddy

Inglett

et al. 2013

Eutrophication: Causes, Consequences and Control

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In wetlands it is still usual to use the same indicators of eutrophication which were developed to study the effects of nutrient enrichment in lakes; however, since hydroecology and biogeochemistry of wetlands is significantly different from lakes, monitoring of these indicators does not allow a good diagnosis of the changes undergone by the wetland ecosystem under nutrient enrichment scenarios. Microbial activities and their respective community responses have been considered as a measure of ecosystem stability and an indicator of ecosystem perturbation through changes on functional properties associated with nutrient cycling. As in most aquatic ecosystems, the addition of a limiting nutrient to wetland ecosystems promotes primary productivity and stimulates microbial processes. As nutrient loading increase, biogeochemical processes in wetlands are altered, changing their concentrations in water and soil, and therefore, nutrient fluxes and cycling. Nutrient enrichment induces changes in soil physicochemical and microbiological characteristics that may then serve as indicators of nutrient enrichment. In this review, a set of microbial community measurements known to be sensitive to nutrient enrichment in aquatic systems, such as extracellular enzyme activities, respiratory activities, microbial biomass C, N, and P, and microbially mediated N and P turnover rates have been used to characterize physiological response of the microbial community to wetland eutrophication. Some indicators as metabolic efficiency and phosphatase activity clearly reflect the main shifts on wetland ecosystem processes induced by nutrient enrichment and may be considered better than those that are currently used to assess the effects of eutrophication. Moreover, the combined use of different ecophysiological measurements such as extracellular enzymatic ratios and microbial biomass under resource allocation models and ecological stoichiometry demonstrates that ecophysiological measures are sensitive indicators of wetland eutrophication. Further studies are needed refining this approach to get the complex biogeochemical variability of the different wetland types, and to move from a sitebased heuristic model to a holistic approach, describing eutrophication patterns in wetlands.

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“…Large amount of information about biological characteristic of the soil is obtained by the determination of DHA in soil (Wolinska and Stepniewska, 2012). Dehydrogenase enzyme is often used as a measure of soil microbial activity and gives account of any disruption caused by pesticides, trace elements or management practices to the soil (Chendrayan et al, 1979;Trevors et al, 1982;McCarthy et al, 1994). Soil DHA is considered to exist in soils as integral parts of intact cells (Taylor et al, 2002).…”

Section: Microbiological Indicesmentioning

confidence: 99%