MdERDL6-mediated glucose efflux to the cytosol promotes sugar accumulation in the vacuole through up-regulating TSTs in apple and tomato

Zhu, Lingcheng; Li, Baiyun; Wu, Limin; Li, Huixia; Wang, Zhengyang; Wei, Xiaoyu; Ma, Baiquan; Zhang, Yanfeng; Ma, Fengwang; Ruan, Yong‐Ling; Li, Mingjun

doi:10.1073/pnas.2022788118

Cited by 94 publications

(82 citation statements)

References 53 publications

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“…17 ), so it was designated as PpERDL16 . Previous studies showed that AtERDL6 in Arabidopsis and MdERDL6-1 in apple are symporter proteins that function in glucose export from the vacuole into the cytosol 69 , 70 , and transgenic Arabidopsis lines overexpressing AtERDL6 showed lower levels of glucose and fructose in leaves as compared to wild type plants 69 . We confirmed the tonoplast localization of the PpERDL16-GFP fusion protein in tobacco leaf cells using the Atγ-TIP-mCherry fusion protein as the positive control (Fig.…”

Section: Resultsmentioning

confidence: 99%

Population-scale peach genome analyses unravel selection patterns and biochemical basis underlying fruit flavor

Guan

et al. 2021

Nat Commun

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A narrow genetic basis in modern cultivars and strong linkage disequilibrium in peach (Prunus persica) has restricted resolution power for association studies in this model fruit species, thereby limiting our understanding of economically important quality traits including fruit flavor. Here, we present a high-quality genome assembly for a Chinese landrace, Longhua Shui Mi (LHSM), a representative of the Chinese Cling peaches that have been central in global peach genetic improvement. We also map the resequencing data for 564 peach accessions to this LHSM assembly at an average depth of 26.34× per accession. Population genomic analyses reveal a fascinating history of convergent selection for sweetness yet divergent selection for acidity in eastern vs. western modern cultivars. Molecular-genetics and biochemical analyses establish that PpALMT1 (aluminum-activated malate transporter 1) contributes to their difference of malate content and that increases fructose content accounts for the increased sweetness of modern peach fruits, as regulated by PpERDL16 (early response to dehydration 6-like 16). Our study illustrates the strong utility of the genomics resources for both basic and applied efforts to understand and exploit the genetic basis of fruit quality in peach.

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Section: Resultsmentioning

confidence: 99%

Population-scale peach genome analyses unravel selection patterns and biochemical basis underlying fruit flavor

Guan

et al. 2021

Nat Commun

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“…It is highly expressed in fruits, and its encoded protein seems to be localized on the tonoplast, where it acts as a low-affinity H + /glucose symporter. In an inverse relation to AtERDL6, the overexpression of MdERDL6-1 in apple and tomato leaves and fruits leads to an increase rather than a decrease in sugar levels (Zhu et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Responsiveness of Early Response to Dehydration Six-Like Transporter Genes to Water Deficit in Arabidopsis thaliana Leaves

et al. 2021

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Drought is one of the main abiotic stresses, which affects plant growth, development, and crop yield. Plant response to drought implies carbon allocation to sink organs and sugar partitioning between different cell compartments, and thereby requires the involvement of sugar transporters (SUTs). Among them, the early response to dehydration six-like (ESL), with 19 members in Arabidopsis thaliana, form the largest subfamily of monosaccharide transporters (MSTs) still poorly characterized. A common feature of these genes is their involvement in plant response to abiotic stresses, including water deficit. In this context, we carried out morphological and physiological phenotyping of A. thaliana plants grown under well-watered (WW) and water-deprived (WD) conditions, together with the expression profiling of 17 AtESL genes in rosette leaves. The drought responsiveness of 12 ESL genes, 4 upregulated and 8 downregulated, was correlated to different water statuses of rosette leaves. The differential expression of each of the tandem duplicated AtESL genes in response to water stress is in favor of their plausible functional diversity. Furthermore, transfer DNA (T-DNA) insertional mutants for each of the four upregulated ESLs in response to water deprivation were identified and characterized under WW and WD conditions. To gain insights into global sugar exchanges between vacuole and cytosol under water deficit, the gene expression of other vacuolar SUTs and invertases (AtTMT, AtSUC, AtSWEET, and AtβFRUCT) was analyzed and discussed.

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“…The TST family genes are involved in vacuolar sugar accumulation in different species, such as Arabidopsis (Schulz et al, 2011; Wormit et al, 2006), sugar beet (Jung et al, 2015), tomato and apple (Zhu et al, 2021). However, none of the previously identified TST genes are stem‐specifically expressed.…”

Section: Discussionmentioning

confidence: 99%

Identification and analysis of stem‐specific promoters from sugarcane and energy cane for oil accumulation in their stems

Wang

Wai

et al. 2021

GCB Bioenergy

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Considerable recent progress has been achieved in bioengineering oil accumulation in the vegetative tissues of plants, opening an opportunity for large scale production of biodiesel, jet fuel, lubricants, and high‐value lipid bioproducts. For the highly productive C4 crops, such as sugarcane, energy cane, Miscanthus, and fiber sorghums, the bulk of the biomass is the stem. However, little success has been made in accumulating oil in the stem. Since engineering a trait with a constitutive promoter often results in pleiotropic effects that counter trait improvement, identification of stem parenchyma‐specific promoters is a prerequisite for efficient use of the ample photoassimilates stored in mature stem parenchyma cells. In this study, we first identified two TST genes encoding homologues of tonoplast sugar transporters that were strongly and almost exclusively expressed in the stems of canes via a combination of RNA‐seq atlas analysis, in silico analysis of a sugarcane genome, phylogenetic analysis, and quantitative PCR analysis. They were further confirmed in the pith parenchyma cells of the mature stem by RNA in situ hybridization. When fused with the β‐Glucuronidase (GUS) reporter gene, the promoters of two alleles, TST2b‐1A and TST2b‐1C, from one TST gene demonstrated that they could drive the GUS expression exclusively in the stem in Arabidopsis.

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MdERDL6-mediated glucose efflux to the cytosol promotes sugar accumulation in the vacuole through up-regulating TSTs in apple and tomato

Cited by 94 publications

References 53 publications

Population-scale peach genome analyses unravel selection patterns and biochemical basis underlying fruit flavor

Population-scale peach genome analyses unravel selection patterns and biochemical basis underlying fruit flavor

Responsiveness of Early Response to Dehydration Six-Like Transporter Genes to Water Deficit in Arabidopsis thaliana Leaves

Identification and analysis of stem‐specific promoters from sugarcane and energy cane for oil accumulation in their stems

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