McCarey-Kaufman (MK) Organ Culture and MK Medium-Shifted Corneas

Effect of transfer of donor corneal tissue from McCarey–Kaufmann medium to Optisol-GS on corneal endothelium

“…The upper time limit was decided to eliminate storage media induced endothelial cell loss in MK media. [ 15 ] The lower limit was chosen as 48 h because it is only by this time, that eye banks either utilize the tissue or feel the need to extend the storage life of the tissue by transferring to a longer-term storage medium.…”

“…Previously, transfer of tissue from MK medium to organ culture is reported[ 15 ] and has not shown a deleterious effect on the endothelium by electron microscopy. To the best of our knowledge, this study is the first to provide us data for evaluating the effectiveness and safety of transferring corneal tissue from MK Medium to Optisol-GS medium.…”

Effect of transfer of donor corneal tissue from McCarey–Kaufmann medium to Optisol-GS on corneal endothelium

“…The upper time limit was decided to eliminate storage media induced endothelial cell loss in MK media. [ 15 ] The lower limit was chosen as 48 h because it is only by this time, that eye banks either utilize the tissue or feel the need to extend the storage life of the tissue by transferring to a longer-term storage medium.…”

“…Previously, transfer of tissue from MK medium to organ culture is reported[ 15 ] and has not shown a deleterious effect on the endothelium by electron microscopy. To the best of our knowledge, this study is the first to provide us data for evaluating the effectiveness and safety of transferring corneal tissue from MK Medium to Optisol-GS medium.…”

Effect of transfer of donor corneal tissue from McCarey–Kaufman medium to Optisol on corneal endothelium

“…The authors did not find any adverse effects both on specular microscopy and transmission electron microscopy. [ 1 ]…”

Donor cornea transfer from Optisol GS to organ culture storage: a two‐step procedure to increase donor tissue lifespan

“…During storage and also during transport and international exchange of donor corneas, the preservation time for tissue in Optisol GS may approximate the upper recommended limit, and transfer to EBOC has been suggested as an option to increase the lifespan of donor corneas (Nelson et al. 1984; Camposampiero et al.…”

“…During storage and also during transport and international exchange of donor corneas, the preservation time for tissue in Optisol GS may Donor cornea transfer from Optisol GS to organ culture storage: a two-step procedure to increase donor tissue lifespan approximate the upper recommended limit, and transfer to EBOC has been suggested as an option to increase the lifespan of donor corneas (Nelson et al 1984;Camposampiero et al 2003;Rijneveld et al 2011). Such a dual approach may increase the pool of available donor tissue (Rijneveld et al 2011) and may, in addition, permit some regeneration of epithelial defects prior to release of tissue for surgery.…”

Effect of transfer of donor corneal tissue from McCarey–Kaufmann medium to Optisol-GS on corneal endothelium

“…The upper time limit was decided to eliminate storage media induced endothelial cell loss in MK media. [ 15 ] The lower limit was chosen as 48 h because it is only by this time, that eye banks either utilize the tissue or feel the need to extend the storage life of the tissue by transferring to a longer-term storage medium.…”

“…Previously, transfer of tissue from MK medium to organ culture is reported[ 15 ] and has not shown a deleterious effect on the endothelium by electron microscopy. To the best of our knowledge, this study is the first to provide us data for evaluating the effectiveness and safety of transferring corneal tissue from MK Medium to Optisol-GS medium.…”

Effect of transfer of donor corneal tissue from McCarey–Kaufman medium to Optisol on corneal endothelium

“…The authors did not find any adverse effects both on specular microscopy and transmission electron microscopy. [ 1 ]…”

Donor cornea transfer from Optisol GS to organ culture storage: a two‐step procedure to increase donor tissue lifespan

“…During storage and also during transport and international exchange of donor corneas, the preservation time for tissue in Optisol GS may approximate the upper recommended limit, and transfer to EBOC has been suggested as an option to increase the lifespan of donor corneas (Nelson et al. 1984; Camposampiero et al.…”

“…During storage and also during transport and international exchange of donor corneas, the preservation time for tissue in Optisol GS may Donor cornea transfer from Optisol GS to organ culture storage: a two-step procedure to increase donor tissue lifespan approximate the upper recommended limit, and transfer to EBOC has been suggested as an option to increase the lifespan of donor corneas (Nelson et al 1984;Camposampiero et al 2003;Rijneveld et al 2011). Such a dual approach may increase the pool of available donor tissue (Rijneveld et al 2011) and may, in addition, permit some regeneration of epithelial defects prior to release of tissue for surgery.…”

Effect of transfer of donor corneal tissue from McCarey–Kaufmann medium to Optisol-GS on corneal endothelium

“…The upper time limit was decided to eliminate storage media induced endothelial cell loss in MK media. [ 15 ] The lower limit was chosen as 48 h because it is only by this time, that eye banks either utilize the tissue or feel the need to extend the storage life of the tissue by transferring to a longer-term storage medium.…”

“…Previously, transfer of tissue from MK medium to organ culture is reported[ 15 ] and has not shown a deleterious effect on the endothelium by electron microscopy. To the best of our knowledge, this study is the first to provide us data for evaluating the effectiveness and safety of transferring corneal tissue from MK Medium to Optisol-GS medium.…”

Effect of transfer of donor corneal tissue from McCarey–Kaufman medium to Optisol on corneal endothelium

“…The authors did not find any adverse effects both on specular microscopy and transmission electron microscopy. [ 1 ]…”

Donor cornea transfer from Optisol GS to organ culture storage: a two‐step procedure to increase donor tissue lifespan

“…During storage and also during transport and international exchange of donor corneas, the preservation time for tissue in Optisol GS may approximate the upper recommended limit, and transfer to EBOC has been suggested as an option to increase the lifespan of donor corneas (Nelson et al. 1984; Camposampiero et al.…”

“…During storage and also during transport and international exchange of donor corneas, the preservation time for tissue in Optisol GS may Donor cornea transfer from Optisol GS to organ culture storage: a two-step procedure to increase donor tissue lifespan approximate the upper recommended limit, and transfer to EBOC has been suggested as an option to increase the lifespan of donor corneas (Nelson et al 1984;Camposampiero et al 2003;Rijneveld et al 2011). Such a dual approach may increase the pool of available donor tissue (Rijneveld et al 2011) and may, in addition, permit some regeneration of epithelial defects prior to release of tissue for surgery.…”