MCB-mediated regulation of cell cycle-specific cdc22 + transcription in fission yeast

Maqbool, Zakia; Kersey, Paul J.; Fantes, Peter A.; McInerny, Christopher

doi:10.1007/s00438-003-0885-4

Cited by 14 publications

(21 citation statements)

References 32 publications

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“…In addition, perturbing the activities of the DSC, Ace2p, and Sep1p transcription factors revealed subsets of functional targets and helped to define the corresponding promoter requirements. Together, our results are consistent with the hypothesis that cell cycle-regulated genes play an important role in cell cycle progression Futcher, 2000;Whitfield et al, 2002;Maqbool et al, 2003).…”

Section: Discussionsupporting

confidence: 81%

“…Cell cycle progression is thus regulated by a wide range of mechanisms such as protein modification, targeted proteolytic degradation, and cell cycle-specific transcription, which has been found to play a pivotal role in controlling cell cycle progression (Maqbool et al, 2003). In the budding yeast Saccharomyces cerevisiae, among a total of ϳ6000 genes, ϳ400 -800 have been found whose transcript levels oscillate during the mitotic cell cycle (Cho et al, 1998;Spellman et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

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Identification of Cell Cycle-regulated Genes in Fission Yeast

Peng

Karuturi

Miller

et al. 2005

MBoC

161

224

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Cell cycle progression is both regulated and accompanied by periodic changes in the expression levels of a large number of genes. To investigate cell cycle-regulated transcriptional programs in the fission yeast Schizosaccharomyces pombe, we developed a whole-genome oligonucleotide-based DNA microarray. Microarray analysis of both wild-type and cdc25 mutant cell cultures was performed to identify transcripts whose levels oscillated during the cell cycle. Using an unsupervised algorithm, we identified 747 genes that met the criteria for cell cycle-regulated expression. Peaks of gene expression were found to be distributed throughout the entire cell cycle. Furthermore, we found that four promoter motifs exhibited strong association with cell cycle phase-specific expression. Examination of the regulation of MCB motifcontaining genes through the perturbation of DNA synthesis control/MCB-binding factor (DSC/MBF)-mediated transcription in arrested synchronous cdc10 mutant cell cultures revealed a subset of functional targets of the DSC/MBF transcription factor complex, as well as certain gene promoter requirements. Finally, we compared our data with those for the budding yeast Saccharomyces cerevisiae and found ϳ140 genes that are cell cycle regulated in both yeasts, suggesting that these genes may play an evolutionarily conserved role in regulation of cell cycle-specific processes. Our complete data sets are available at http://giscompute.gis.a-star.edu.sg/ϳgisljh/CDC.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Identification of Cell Cycle-regulated Genes in Fission Yeast

Peng

Karuturi

Miller

et al. 2005

MBoC

161

224

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“…+ and plo1 + mRNA levels detected by northern blot analysis. mRNA levels of cdc22 + , a known G1-S expressed transcript independent of PBF-PCB control (Maqbool et al, 2003), were also detected to confirm synchrony in each experiment. (B)Separate asynchronous populations of mid1 + (wild-type), mid1 and mid1 clp1 (two isolates) cells were grown and samples taken for northern blot analysis.…”

Section: The Cell Cycle Role Of Mid1p In the Nucleusmentioning

confidence: 94%

“…5A). The effect of mid1 was specific to M-G1 transcribed genes because cdc22 + , a gene expressed at G1-S under MCB-MBF control (Maqbool et al, 2003), was unaffected, with similar narrow peaks of expression in both mid1 and wild-type cells. The fact that the absence of Mid1p causes gene expression outside the M-G1 interval suggests that this protein has a repressive role in controlling transcription.…”

Section: Mid1p Is Required For Correct M-g1 Specific Gene Expressionmentioning

confidence: 99%

Mid1p-dependent regulation of the M–G1 transcription wave in fission yeast

Agarwal

Papadopoulou

Mayeux

et al. 2010

Journal of Cell Science

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SummaryThe control of gene expression at certain times during the mitotic cell division cycle is a common feature in eukaryotes. In fission yeast, at least five waves of gene expression have been described, with one transcribed at the M-G1 interval under the control of the PBF transcription factor complex. PBF consists of at least three transcription factors, two forkhead-like proteins Sep1p and Fkh2p, and a MADS box-like protein Mbx1p, and binds to PCB motifs found in the gene promoters. Mbx1p is under the direct control of the polo-like kinase Plo1p and the Cdc14p-like phosphatase Clp1p (Flp1p). Here, we show that M-G1 gene expression in fission yeast is also regulated by the anillin-like protein, Mid1p (Dmf1p). Mid1p binds in vivo to both Fkh2p and Sep1p, and to the promoter regions of M-G1 transcribed genes. Mid1p promoter binding is dependent on Fkh2p, Plo1p and Clp1p. The absence of mid1 + in cells results in partial loss of M-G1 specific gene expression, suggesting that it has a negative role in controlling gene expression. This phenotype is exacerbated by also removing clp1 + , suggesting that Mid1p and Clp1p have overlapping functions in controlling transcription. As mid1 + is itself expressed at M-G1, these observations offer a new mechanism whereby Mid1p contributes to controlling cell cyclespecific gene expression as part of a feedback loop.

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“…3A). All of the 8 motifs whose width ranged from 6 to 9 nucleotides were found to be related to a motif known as MCB2 (19,38,47). We therefore designated these motifs MCB2-like motifs.…”

Section: Methodsmentioning

confidence: 99%

Deconvolution of Chromatin Immunoprecipitation-Microarray (ChIP-chip) Analysis of MBF Occupancies Reveals the Temporal Recruitment of Rep2 at the MBF Target Genes

et al. 2011

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MBF (or DSC1) is known to regulate transcription of a set of G 1 /S-phase genes encoding proteins involved in regulation of DNA replication. Previous studies have shown that MBF binds not only the promoter of G 1 /S-phase genes, but also the constitutive genes; however, it was unclear if the MBF bindings at the G 1 /S-phase and constitutive genes were mechanistically distinguishable. Here, we report a chromatin immunoprecipitation-microarray (ChIP-chip) analysis of MBF binding in the Schizosaccharomyces pombe genome using high-resolution genome tiling microarrays. ChIP-chip analysis indicates that the majority of the MBF occupancies are located at the intragenic regions. Deconvolution analysis using Rpb1 ChIP-chip results distinguishes the Cdc10 bindings at the Rpb1-poor loci (promoters) from those at the Rpb1-rich loci (intragenic sequences). Importantly, Res1 binding at the Rpb1-poor loci, but not at the Rpb1-rich loci, is dependent on the Cdc10 function, suggesting a distinct binding mechanism. Most Cdc10 promoter bindings at the Rpb1-poor loci are associated with the G 1 /S-phase genes. While Res1 or Res2 is found at both the Cdc10 promoter and intragenic binding sites, Rep2 appears to be absent at the Cdc10 promoter binding sites but present at the intragenic sites. Time course ChIP-chip analysis demonstrates that Rep2 is temporally accumulated at the coding region of the MBF target genes, resembling the RNAP-II occupancies. Taken together, our results show that deconvolution analysis of Cdc10 occupancies refines the functional subset of genomic binding sites. We propose that the MBF activator Rep2 plays a role in mediating the cell cycle-specific transcription through the recruitment of RNAP-II to the MBF-bound G 1 /S-phase genes.

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MCB-mediated regulation of cell cycle-specific cdc22 + transcription in fission yeast

Cited by 14 publications

References 32 publications

Identification of Cell Cycle-regulated Genes in Fission Yeast

Identification of Cell Cycle-regulated Genes in Fission Yeast

Mid1p-dependent regulation of the M–G1 transcription wave in fission yeast

Deconvolution of Chromatin Immunoprecipitation-Microarray (ChIP-chip) Analysis of MBF Occupancies Reveals the Temporal Recruitment of Rep2 at the MBF Target Genes

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