Maturation of murine bone marrow dendritic cells induced by acidic Ginseng polysaccharides

Wang, Zuozhou; Meng, Jingjuan; Xia, Yanjie; Meng, Yiming; Du, Lin; Zhang, Zhenjie; Wang, Enhua; Shan, Fengping

doi:10.1016/j.ijbiomac.2012.11.009

Cited by 23 publications

(11 citation statements)

References 22 publications

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“…Moreover, LPS-treated BMDCs have changed its surface much more than the other treatments. Our results are in accordance with previous reports stating that the emergence of cell surface roughness and protrusion is considered to be associated with BMDCs maturation (Li & Lu, 2009;Wang et al, 2013;Xing et al, 2011).…”

Section: Discussionsupporting

confidence: 94%

“…Evidence proved that upon antigen or pathogen recognition, DCs release a large amount of cytokines such as TNF-α, IL-6, IL-1β, MCP-1, and IL-12 (An, Kim, Lee, Park, & Chang, 2014;Tsao, Kuan, Wang, & Sheu, 2013;Wang et al, 2013;Xing et al, 2011;Yu et al, 2009), thereby limiting the spread of infection and mediating helper T cells activation (Banchereau et al, 2000). TNF-α, IL-6, and IL-1β are pro-inflammatory cytokines participating in the resistance against infectious agents and tumour cells, while IL-12 has a role in enhancing the T-cell responsiveness, especially in promoting the helper T cell differentiation.…”

Section: Discussionmentioning

confidence: 99%

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Jellyfish collagen stimulates maturation of mouse bone marrow-derived dendritic cells

Putra

Nishi

Shiraishi

et al. 2015

Journal of Functional Foods

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Jellyfish collagen stimulates maturation of mouse bone marrow-derived dendritic cells

Putra

Nishi

Shiraishi

et al. 2015

Journal of Functional Foods

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“…Among them, the polysaccharides have been shown to have effects on immunologic defense functions [ 5 , 6 ]. The mechanisms of action have not been fully elucidated at the molecular level, but these have been shown to activate many of the diverse types of cells in the immune system [ 7 , 8 ] and stimulate production of various cytokines [ 9 - 12 ]. A number of pharmacologically active polysaccharides with molecular weights ranging from 3500 to 160,000 Da have been identified.…”

Section: Introductionmentioning

confidence: 99%

A 14-week randomized, placebo-controlled, double-blind clinical trial to evaluate the efficacy and safety of ginseng polysaccharide (Y-75)

2014

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BackgroundThe Y-75 (Ginsan) acidic polysaccharide from Korean Panax ginseng has been shown to function as an immunomodulatory molecule. However, the efficacy of Y-75 has not been evaluated in clinical trial.MethodsWe verified Y-75 (6 g/day) for safety and immune efficacy in 72 healthy volunteers aged 50–75 years using a randomized, placebo-controlled, parallel, double-blind study. The activities of natural killer (NK) cells and peripheral blood phagocytes, as well as serum levels of monocyte-derived mediators, were assessed before and after administration for 8 and 14 weeks. This trial is registered at ClinicalTrials.gov (NCT02161198).ResultsY-75 significantly enhanced NK cell cytotoxic activity by 35.2% and 40.2% from baseline after administration for 8 and 14 weeks, respectively. The phagocytic activity of peripheral blood cells was also significantly increased by 25.2% and 39.4% and serum level of TNF-α by 38.2% and 44.5% after treatment for 8 and 14 weeks, respectively. Differences in the efficacy of variables compared to the placebo group were also significant. Administration of Y-75 was well tolerated without treatment-related adverse events or alteration of complete blood cell count or blood chemistry over the entire study period.ConclusionY-75 was shown to be a safe and potentially effective natural alternative for enhancing immune function.

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“…Previous reports showed that GR can induce the activation of mouse bone marrow-derived DCs (BMDCs) [ 17 ]. We therefore assessed whether GB can also induce the activation or maturation of BMDCs in vitro .…”

Section: Resultsmentioning

confidence: 99%

Ginseng Berry Extract Promotes Maturation of Mouse Dendritic Cells

et al. 2015

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Ginseng extract has been shown to possess certain anti-virus, anti-tumor and immune-activating effects. However, the immunostimulatory effect of ginseng berry extract (GB) has been less well characterized. In this study, we investigated the effect of GB on the activation of mouse dendritic cells (DCs) in vitro and in vivo. GB treatment induced up-regulation of co-stimulatory molecules in bone marrow-derived DCs (BMDCs). Interestingly, GB induced a higher degree of co-stimulatory molecule up-regulation than ginseng root extract (GR) at the same concentrations. Moreover, in vivo administration of GB promoted up-regulation of CD86, MHC class I and MHC class II and production of IL-6, IL-12 and TNF-α in spleen DCs. GB also promoted the generation of Th1 and Tc1 cells. Furthermore, Toll like receptor 4 (TLR4) and myeloid differentiation primary response 88 (MyD88) signaling pathway were essential for DC activation induced by GB. In addition, GB strongly prompted the proliferation of ovalbumin (OVA)-specific CD4 and CD8 T cells. Finally, GB induced DC activation in tumor-bearing mice and the combination of OVA and GB treatment inhibited B16-OVA tumor cell growth in C57BL/6 mice. These results demonstrate that GB is a novel tumor therapeutic vaccine adjuvant by promoting DC and T cell activation.

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Maturation of murine bone marrow dendritic cells induced by acidic Ginseng polysaccharides

Cited by 23 publications

References 22 publications

Jellyfish collagen stimulates maturation of mouse bone marrow-derived dendritic cells

Jellyfish collagen stimulates maturation of mouse bone marrow-derived dendritic cells

A 14-week randomized, placebo-controlled, double-blind clinical trial to evaluate the efficacy and safety of ginseng polysaccharide (Y-75)

Ginseng Berry Extract Promotes Maturation of Mouse Dendritic Cells

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