Matrix-comprehensive in-house validation and robustness check of a confirmatory method for the determination of four nitrofuran metabolites in poultry muscle and shrimp by LC–MS/MS

Bock, C.; Gowik, Petra; Stachel, Carolin S.

doi:10.1016/j.jchromb.2007.05.044

Cited by 45 publications

(19 citation statements)

References 15 publications

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“…LC-MS/MS has been utilised in studies examining nitrofuran parent drugs and their metabolite contamination in eggs (Finzi et al, 2005;Szilagyi and de la Calle, 2006;Bock et al, 2007a), poultry (Finzi et al, 2005;Verdon et al, 2007), porcine tissue (McCracken et al, 1995;McCracken and Kennedy, 1997a;Leitner et al, 2001) as well as fish feed (Hu et al, 2007).…”

Section: Instrumental Methods For Nitrofuran Determinationmentioning

confidence: 99%

“…The use of radioactive labelled internal standards enables the correction of errors resulting from matrix interference, chromatography and detection. Standard solutions of nitrofuran metabolites in methanol are stable for up to one year at 4°C (in the dark) and extracted samples can be stored up to three days prior to analysis (Bock et al, 2007a). LC-MS/MS is highly sensitive and the sample matrix can often interfere with the analyte signal.…”

Section: Instrumental Methods For Nitrofuran Determinationmentioning

confidence: 99%

See 1 more Smart Citation

Nitrofuran antibiotics: a review on the application, prohibition and residual analysis

Vass¹,

Hruška²,

Fránek³

2008

Vet. Med.

229

130

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Nitrofuran antibiotics, employed for the treatment of bacterial diseases in livestock production, were banned from use in the European Union (EU) in 1995 due to concerns about the carcinogenicity of their residues in edible tissue. This review provides an overview of nitrofuran toxicity, metabolism, and also specific aspects of legislation surrounding their prohibition. Special attention is devoted to semicarbazide -a nitrofuran metabolite and food contaminant. Analytical procedures for nitrofuran analysis in various matrices and validation requirements for screening and confirmation methods with respect to EU regulations are also reviewed.Keywords: nitrofurans; tissue bound metabolites; semicarbazide; bioavailability; mutagenicity; legislation; sample preparation; validation; detection methods List of abbreviations AHD = 1-aminohydantoin; AOZ = 3-amino-2-oxazolidinone; AMOZ = 3-amino-5-morpholino-methyl-1,3-oxazolidinone; CC α = decision limit; CC β = detection capability; EC = European Commission; EFSA = European Food Safety Authority; ELISA = enzyme linked immuno-adsorbent assay; ESI = electro-spray ionisation; EU = European Union; FTD = furaltadone; FZD = furazolidone; HPLC = high performance liquid chromatography; IC = inhibition concentration; LC = liquid chromatography; LOD = limit of detection; MS = mass spectrometry; NFT = nitrofurantoin; NFZ = nitrofurazone; NP = nitrophenyl; NPAHD = 3-(2-nitrobenzylidenamino)-2,4-imidazolidinedione); NPAMOZ = 5-(morpholinomethyl)-3-(2-nitrobenzylidenamino)-2-oxazolidinone); NPAOZ = [3-(2-nitrobenzylidenamino)-2-oxazolidinone]; NPSEM = 3[(2-nitrophenyl)methylene]-hydrazinecarboxamide; o-NBA = ortho-nitrobenzaldehyde; RASFF = Rapid Alert System for Food and Feed; SE = solvent extraction; SEM = semicarbazide; SPE = solid phase extraction; UV = ultraviolet

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Section: Instrumental Methods For Nitrofuran Determinationmentioning

confidence: 99%

Section: Instrumental Methods For Nitrofuran Determinationmentioning

confidence: 99%

Nitrofuran antibiotics: a review on the application, prohibition and residual analysis

Vass¹,

Hruška²,

Fránek³

2008

Vet. Med.

229

130

View full text Add to dashboard Cite

show abstract

“…Owing to its being a micromolecule, the space structure of metabolite is transformed accompanying redistribution of electron cloud after introduction of aromatic ring. Generally, determination and identification of nitrofuran metabolite in instrumental analysis and ELISA is carried out by derivatization of analyte with onitrobenzaldehyde (Cooper et al, 2005;Bock et al, 2007). In contrast, we used PAHD as the target analyte by derivatization with benzaldehyde instead; as a result the sensitivity of our assay was improved.…”

Section: Sample Analysismentioning

confidence: 99%

Development of an immunochromatographic assay for rapid detection of 1‐Aminohydantoin in urine specimens

Liu

et al. 2008

Biomedical Chromatography

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A rapid immunochromatographic assay was developed and validated for detection of 1-aminohydantoin (AHD) in urine specimens. Colloidal gold-labeled polyclonal antibody specific to AHD derivative was used as the marker; based on the competitive reactivity theory, the metabolite of nitrofurantoin after derivatization with benzaldehyde would compete with carboxyphenyl AHD derivative-conjugated ovalbumin. The test strip could efficaciously detect the novel analyte with a visual detection limit of 10 ng mL(-1) and high specificity. The reliability of the assay was determined by testing 80 standard samples comparing with enzyme-linked immunosorbent assay. The semi-quantitative detection was accomplished in less than 15 min with low cost, especially for requirements of rapid and simple screening. This is the first publication of an immunochromatographic assay for detection of nitrofuran residues.

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“…Two approaches can be adopted in NF analysis, namely, extraction of total (free and bound) or bound residues. The total residue approach has been applied by several groups and offers advantages in terms of speed as it eliminates the need for time-consuming washing of tissue [242][243][244][245][246][247]. NF metabolites (AHD, AOZ, AMOZ and SEM) are usually released from tissue by acid hydrolysis and derivatised (overnight incubation with HCl and 2-nitrobenzaldehyde (NBA)).…”

Section: Nitrofuransmentioning

confidence: 99%

Current trends in sample preparation for growth promoter and veterinary drug residue analysis

Kinsella

O’Mahony

Malone

et al. 2009

Journal of Chromatography A

179

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A comprehensive review is presented on the current trends in sample preparation for isolation of veterinary drugs and growth promotors from foods. The objective of the review is to firstly give an overview of the sample preparation techniques that are applied in field. The review will focus on new techniques and technologies, which improve efficiency and coverage of residues. The underlying theme to the paper is the developments that have been made in multi-residue methods and particularly multi-class methods for residues of licensed animal health products, which have been developed in the last couple of years. The role of multi-class methods is discussed and how they can be accommodated in future residue surveillance.

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Matrix-comprehensive in-house validation and robustness check of a confirmatory method for the determination of four nitrofuran metabolites in poultry muscle and shrimp by LC–MS/MS

Cited by 45 publications

References 15 publications

Nitrofuran antibiotics: a review on the application, prohibition and residual analysis

Nitrofuran antibiotics: a review on the application, prohibition and residual analysis

Development of an immunochromatographic assay for rapid detection of 1‐Aminohydantoin in urine specimens

Current trends in sample preparation for growth promoter and veterinary drug residue analysis

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