Matricaria Recutita Extract (Chamomile) to reduce Candida Albicans and Entrobacter Cloacae biofilms: in vitro study

Abstract

Objective: This research study aimed at evaluating the inhibitory activity of Matricaria recutira (chamomile) hydroalcoholic extract on Candida albicans and Enterobacter cloacae biofilms. Methods: C. albicans and E. cloacae biofilms with thirty-hour formation were submitted, for five minutes, to 100, 200 and 300 mg / mL of M. recutita hydroalcoholic extract, chlorhexidine digluconate 0.12% (Periogard® - inhibition control) or sterile distilled water (growth control). Subsequently, they were washed and divided into two groups to determine the microbial viability: G/UFC - counting of colony forming units (cfu) in agar and G/DNA - quantification of viable DNA with violet crystal dye by spectrophotometry. Results: M. recutita extract at 300 mg/mL reduced significantly (p <0.01) the E. cloacae cfu/mL number in biofilm with results similar to chlorhexidine 0.12%, while extracts at 100 and 200 mg/mL did not have the same effectiveness. The amount of E. cloacae viable DNA was reduced (p <0.05) in all the M. recutita extract concentrations and chlorhexidine. There was no significant difference (p = 0.565) in the cfu/mL number or in the amount of viable DNA (p = 0.8094) in C. albicans biofilm when compared to untreated biofilm (control) or, even, between the extracts when compared to each other or to chlorhexidine 0.12%. Conclusion: 300 mg/mL M. recutita extract reduced significantly the E. cloacae biofilm but not the C. albicans, both with a similar result to chlorhexidine 0.12% (Periogar®).

Effects of Plant Extracts on Bacterial Isolates from Infections of the Female Genital Tract

“…However, it did not demonstrate any impact on the amount of viable DNA in C. albicans biofilm. [3]. By using the agar diffusion method, the antimicrobial activity of chamomile essential oil was examined, and it was discovered to have a considerable antibacterial impact, with inhibition zones of 13.33 mm to 40.00 mm in diameter (on Listeria monocytogenes) (on S. aureus).…”

“…Plants have an essential role in the treatment of fungal and bacterial illnesses. Several studies show that plant extracts (Chamomile and Thyme, Marjoram, Peat, Thuja) have antifungal and antibacterial activity against Candida albicans and bacterial infection (Staphylococcus aureus, Escherichia coli, and Staphylococcus epidermidis) [2][3][4][5][6].…”