Maternal testosterone and reproductive outcome in a rat model of obesity

Arnon, Liat; Hazut, Noa; Tabachnik, Tzlil; Weller, Aron; Koren, Lee

doi:10.1016/j.theriogenology.2016.03.033

Cited by 13 publications

(25 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Surprisingly, however, no attention has been given to the role(s) of testosterone in female bears as underscored by the many studies of reproduction, or reproductive hormones, in a variety of bear species over the past 40 years in which testosterone dynamics or differences are studied in males only, and estradiol and progesterone are studied in females only. Even with research involving humans, testosterone is traditionally associated with, and studied in males, whereas studies of females have focused on the effects of progesterone and estradiol (Arnon et al ., 2016). Our findings from this study contrast to some degree with those of Bryan et al .…”

Section: Discussionmentioning

confidence: 99%

The quantification of reproductive hormones in the hair of captive adult brown bears and their application as indicators of sex and reproductive state

Cattet

Stenhouse

Janz

et al. 2017

Conservation Physiology

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

The quantification of reproductive hormones in the hair of captive adult brown bears and their application as indicators of sex and reproductive state

Cattet

Stenhouse

Janz

et al. 2017

Conservation Physiology

View full text Add to dashboard Cite

show abstract

“…Some studies that demonstrated significant differences in rodent hair corticosterone following up to 5 weeks of experimental manipulation analyzed only new hair growth [29, 30]. In contrast, other studies that examined hair corticosterone in rodents from old hair growth did not reveal significant differences between experimental groups [32, 48]. Because the complete hair growth cycle in mice is relatively short, approximately 3 weeks [49], we were able to determine whether prior hair removal and collection of new hair growth was necessary to detect changes in HPA activity over the 4 week study period.…”

Section: Discussionmentioning

confidence: 99%

Hair corticosterone measurement in mouse models of type 1 and type 2 diabetes mellitus

Erickson

Browne

Lucki

2017

Physiology & Behavior

View full text Add to dashboard Cite

In diabetes, glucocorticoid secretion increases secondary to hyperglycemia and is associated with an extensive list of disease complications. Levels of cortisol in humans, or corticosterone in rodents, are usually measured as transitory biomarkers of stress in blood or saliva. Glucocorticoid concentrations accumulate in human or animal hair over weeks and could more accurately measure the cumulative stress burden of diseases like chronic diabetes. In this study, corticosterone levels were measured in hair in verified rodent models of diabetes mellitus. To induce type 1 diabetes, C57BL/6J mice were injected with streptozotocin and blood and hair samples were collected 28 days following induction. Leptin receptor deficient (db/db) mice were used as a spontaneous model of type 2 diabetes and blood and hair samples were collected at 8 weeks of age, after the development of hyperglycemia and obesity. Corticosterone levels from serum, new growth hair and total growth hair were analyzed using an enzyme immunoassay. Corticosterone levels in new growth hair and serum were significantly elevated in both models of diabetes compared to controls. In contrast, corticosterone levels in old hair growth did not differ significantly between diabetic and non-diabetic animals. Thus, hair removal and sampling of new hair growth was a more sensitive procedure for detecting changes in hair corticosterone levels induced by periods of hyperglycemia lasting for 4 weeks in mice. These results validate the use of hair to measure long-term changes in corticosterone induced by diabetes in rodent models. Further studies are now needed to validate the utility of hair cortisol as a tool for measuring the stress burden of individuals with diabetes and for following the effects of long-term medical treatments.

show abstract

“…In male OLETF rats, we [20][21][22][23][24] and others [25] demonstrated vascular dysfunction in several arteries. Conversely, in female OLETF rats, although reproductive or metabolic dysfunction were reported [19,26,27], we recently o b s e r v e d t h a t i m p a i r e d e n d o t h e l i u m -d e r i v e d , hyperpolarization-mediated relaxation was impaired in superior mesenteric arteries for this animal model [28]; no study has focused on the relationship between vascular function and nucleotides in female OLETF rats.…”

Section: Introductionmentioning

confidence: 99%

UDP-induced relaxation is enhanced in aorta from female obese Otsuka Long–Evans Tokushima Fatty rats

Kobayashi

Matsumoto

Ando

et al. 2017

Purinergic Signalling

View full text Add to dashboard Cite

Uridine 5′-diphosphate (UDP) plays an important role in controlling vascular tone; however, UDP-mediated response in metabolic syndromes, including obesity and type 2 diabetes in females, remains unclear. In this study, we investigated UDP-mediated response in the aorta of female obese Otsuka Long-Evans Tokushima Fatty (OLETF) rats and control Long-Evans Tokushima Otsuka (LETO) rats. In OLETF rat aortas precontracted by phenylephrine (PE) (vs. LETO), (1) UDP-induced relaxation was increased, whereas acetylcholine (ACh)-induced relaxation was decreased; (2) no UDP-or ACh-induced relaxations were observed in endothelial denudation, whereas UDP-induced small contraction was observed; and (3) N G -nitro-L-arginine [L-NNA, a nitric oxide (NO) synthase inhibitor] eliminated UDP-induced relaxation and small contraction, whereas caused contrasting responses by ACh, including slight relaxations (LETO) and contractions (OLETF). Indomethacin, a cyclooxygenase inhibitor, eliminated the difference in UDP-and ACh-induced relaxations between the groups by increased UDP-induced relaxation in the LETO group and increased ACh-induced relaxation in the OLETF group. MRS2578, a P2Y 6 receptor antagonist, eliminated the difference in UDP-induced relaxations between the groups by decreasing UDP-induced relaxation in the OLETF group. MRS2578 had no effect on UDP-induced contraction in endothelium-denuded aortas. Therefore, these findings demonstrate opposite trends of relaxations by UDP and ACh in OLETF and LETO rat aortas. These differences may be attributed to the imbalance between NO and vasoconstrictor prostanoids upon stimulations. Increased UDP-induced relaxation in OLETF rat aorta may be caused by the activation of endothelial MRS2578-sensitive P2Y 6 receptor.

show abstract

Maternal testosterone and reproductive outcome in a rat model of obesity

Cited by 13 publications

References 38 publications

The quantification of reproductive hormones in the hair of captive adult brown bears and their application as indicators of sex and reproductive state

The quantification of reproductive hormones in the hair of captive adult brown bears and their application as indicators of sex and reproductive state

Hair corticosterone measurement in mouse models of type 1 and type 2 diabetes mellitus

UDP-induced relaxation is enhanced in aorta from female obese Otsuka Long–Evans Tokushima Fatty rats

Contact Info

Product

Resources

About