Maternal plasma antioxidant status in the first trimester of pregnancy and development of obstetric complications

Ramiro-Cortijo, David; Herrera, Teresa; Rodríguez-Rodríguez, Pilar; Pablo, Ángel L. López de; Calle, María de la; López-Giménez, María Rosario; Urda, Ana Isabel Mora; Gutiérrez-Arzapalo, Perla Yareli; Gómez-Rioja, Rubén; Aguilera, Yolanda; Martín‐Cabrejas, Maria A.; Condezo-Hoyos, Luis; González, M. C.; Montero, Pilar; Moreno‐Jiménez, Bernardo; Arribas, Silvia M.

doi:10.1016/j.placenta.2016.08.090

Cited by 28 publications

(32 citation statements)

References 41 publications

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“…It is well documented that oxidative stress prevails during first trimester of pregnancy due to increased reactive oxygen species that influ-Open Journal of Obstetrics and Gynecology ence a number of functions such as angiogenesis, remodelling of the spiral arteries, cell permeability and vascularisation during embryogenesis and placental development [45]. This oxidative stress has been shown to be compensated by the parallel elevation of antioxidant defences and a defective response in this oxidant stimulus may be an essential component in the aetiology of preeclampsia [46], thus an intricate balance between oxidants and antioxidants is vital in early pregnancy [47].…”

Section: Discussionmentioning

confidence: 99%

Maternal Serum Biochemical Indicators of Trophoblastic Cell and Endothelial Function at First Trimester of Pregnancy

Gadde¹,

Dayanand²,

Sheela³

2018

OJOG

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Background: Preeclampsia is a worldwide pregnancy complication, and early identification of patients with an increased risk is one of the key goals in obstetrics. First trimester screening is crucial over the second trimester for understanding the early onset of the disorder, with basal levels of the biochemical parameters associated with the underlying placentation process. Objective: The study aims to assess the levels of serum biochemical markers in pregnant women at first trimester, to evaluate statistical significance and correlation of the values in support of trophoblastic cell integrity, endothelial function and oxidative stress. Materials and Methods: A longitudinal study was conducted on 86 pregnant women of age group 20 -35 years, Primigravida with singleton pregnancy who visited prenatal check up between 11 -13 weeks of gestation. Maternal sera was collected for screening Placental protein 13 (PP13), Caspase 3, Asymmetric dimethylarginine (ADMA), Nitric oxide (NO) by ELISA. Xanthine oxidase (XO) activity was assayed spectrophotometrically. Calcium and Uric acid (UA) were measured by dry chemistry analyser. Results: The mean ± SD values for mean arterial pressure (MAP) are 108.4 ± 18.9, UA 2.01 ± 0.85, Total oxidant status (TOS) 12.83 ± 5.17, Total antioxidant capacity (TAC) 24.

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Section: Discussionmentioning

confidence: 99%

Maternal Serum Biochemical Indicators of Trophoblastic Cell and Endothelial Function at First Trimester of Pregnancy

Gadde¹,

Dayanand²,

Sheela³

2018

OJOG

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“…Total thiol groups. Plasma thiols were assessed by a modification of the method based on 5,5 -dithiobis (2-nitrobenzoic acid) assay, adapted by us to a microplate reader, as previously described [29]. The absorbance was measured at 412 nm, and thiol content was expressed as nM GSH/mg of protein.…”

Section: Plasma Parameters Of Oxidative Statusmentioning

confidence: 99%

“…Calculation of oxidative status scores. The antioxidant score (Antiox-S) was calculated for each subject taking into account the abovementioned antioxidant parameters in plasma (GSH, total thiol groups, and catalase activity) using the statistical methodology previously described [29]. Similarly, the score related to oxidative damage (Proxy-S) was also calculated from MDA and total carbonyl groups.…”

Section: Plasma Parameters Of Oxidative Statusmentioning

confidence: 99%

“…The present study demonstrates that neonates receiving ARA:DHA 2:1 supplementation increased plasma levels of some endogenous antioxidants and the Antiox-S score. We previously used this parameter in humans with several pathologies associated with oxidative stress, namely, pregnancy complication [29], venous insufficiency [26], and hypertension [40]. This score is useful to assess global antioxidant status when individual parameters are highly variable.…”

Section: Effect Of Supplementation On Oxidative Statusmentioning

confidence: 99%

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Plasma Oxidative Status in Preterm Infants Receiving LCPUFA Supplementation: A Pilot Study

et al. 2020

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After birth, preterm infants are deficient in arachidonic acid (ARA), docosahexaenoic acid (DHA), and antioxidants, increasing their risk of oxidative stress-related pathologies. The principal aim was to evaluate if supplementation with long-chain polyunsaturated fatty acids (LCPUFAs) improves antioxidant defenses. In total, 21 preterm infants were supplemented with ARA and DHA in a 2:1 ratio (ARA:DHA-S) or with medium-chain triglycerides (MCT-S). Plasma n-3 and n-6 LCPUFAs were measured at birth, postnatal day 28, and 36 weeks of postmenstrual age (36 WPA) by gas chromatography–mass spectroscopy. Plasma antioxidants (glutathione (GSH), catalase, and thiols) and oxidative damage biomarkers (malondialdehyde (MDA), carbonyls) were analyzed at the same time points by spectrophotometry, and scores of antioxidant status (Antiox-S) and oxidative damage (Proxy-S) were calculated. At 36 WPA, linoleic acid (LA) and dihomo-γ-linolenic acid (DGLA) were decreased in ARA:DHA-S compared to the MCT-S group (LA: ARA:DHA-S = 18.54 ± 1.68, MCT-S = 22.80 ± 1.41; p = 0.018; DGLA: ARA:DHA-S = 1.68 ± 0.38, MCT-S = 2.32 ± 0.58; p = 0.018). Furthermore, α-linolenic acid (ALA) was increased in ARA:DHA-S (ARA:DHA-S = 0.52 ± 0.33, MCT-S = 0.22 ± 0.10; p = 0.018). Additionally, LA:DHA ratio was decreased in the ARA:DHA-S compared to control group (ARA:DHA-S = 6.26 ± 2.35, MCT-S = 8.21 ± 2.65; p = 0.045). By the end of supplementation (36 WPA), catalase, thiol groups, and Antiox-S were significantly higher in neonates receiving ARA:DHA-S compared to those receiving MCT-S, with no differences in oxidative stress biomarkers. In conclusion, ARA:DHA supplementation in preterm neonates resulted in an overall improvement in antioxidant to oxidant balance and a decrease in early fatty acid precursors of the n-6 relative to the n-3 pathway. These effects may reduce oxidative stress and inflammation.

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“…We focused on maternal oxidative status, since the maintenance of an adequate balance between reactive oxygen species (ROS) and antioxidants is critical in pregnancy [9], and oxidative stress is linked to maternofetal complications and deficient fetal growth [10,11]. We have previous evidence that a poor antioxidant status in early pregnancy is associated with the development of maternal complications, including preeclampsia, gestational hypertension, and gestational diabetes [12]. Moreover, melatonin is a relevant antioxidant hormone directly acting as a ROS scavenger, also able to increase the expression of several antioxidant enzymes [13], and with important roles in pregnancy [14,15].…”

Section: Introductionmentioning

confidence: 99%

Maternal Antioxidant Status in Early Pregnancy and Development of Fetal Complications in Twin Pregnancies: A Pilot Study

et al. 2020

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Twin pregnancies are increasing due to the rise in mothers’ childbearing age and have a higher risk of fetal growth restriction (FGR) and prematurity. Therefore, early prediction of these events is important. Our aim was to analyze in the first trimester of pregnancy a possible association between antioxidants, including melatonin, in maternal plasma and the development of fetal complications in twin pregnancies. A single-center, prospective, and observational study was performed in 104 twin-pregnant women. A blood sample was extracted between the 9th and the 11th week of gestation, and plasma was obtained. Antioxidants (thiols, reduced glutathione, phenolic compounds, catalase, superoxide dismutase) and oxidative damage biomarkers (carbonyl groups and malondialdehyde) were assessed by spectrophotometry, and global scores were calculated from these parameters (Antiox-S, Prooxy-S). Melatonin and cortisol were evaluated by a competitive immunoassay. In the first trimester of pregnancy, Antiox-S was significantly lower in women who developed FGR compared to those with normal fetal growth; plasma melatonin was significantly lower in women with preterm compared to those with full-term births and exhibited a positive correlation with birth weight. Maternal cortisol showed a negative correlation with birth weight. We conclude that, for twin gestations, maternal plasma antioxidant status and melatonin could be potential biomarkers to be included in algorithms to predict FGR and preterm labor.

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Maternal plasma antioxidant status in the first trimester of pregnancy and development of obstetric complications

Cited by 28 publications

References 41 publications

Maternal Serum Biochemical Indicators of Trophoblastic Cell and Endothelial Function at First Trimester of Pregnancy

Maternal Serum Biochemical Indicators of Trophoblastic Cell and Endothelial Function at First Trimester of Pregnancy

Plasma Oxidative Status in Preterm Infants Receiving LCPUFA Supplementation: A Pilot Study

Maternal Antioxidant Status in Early Pregnancy and Development of Fetal Complications in Twin Pregnancies: A Pilot Study

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