Maternal immunomodulation of the offspring's immunological system

Campos, Sylvia Maria Nicolau; Oliveira, Victor De; Lessa, Leonardo; Vita, Melissa; Conceição, Marcia; Andrade, Luiz Antonio Botelho; Teixeira, Gerlinde Agate Platais Brasil

doi:10.1016/j.imbio.2014.07.001

Cited by 10 publications

(15 citation statements)

References 51 publications

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“…In this work, along with the assessment of clinical aspects during the induction of food allergy and gut inflammation, gut histopathology was performed. Similar to previous findings of our group, the results obtained led to an array of histological alterations spanning from normal histology (control animals) to severe inflammation of the duodenum (allergic group) . The pathophysiological changes induced by the inflammatory response led to alterations both in the cell count and in the villus architecture.…”

Section: Discussionsupporting

confidence: 87%

“…Similar to previous findings of our group, the results obtained led to an array of histological alterations spanning from normal histology (control animals) to severe inflammation of the duodenum (allergic group). 19,40,41 The pathophysiological changes induced by the inflammatory response led to alterations both in the cell count and in the villus architecture. No animal presented classical anaphylaxis symptoms, such as convulsions, wheezing, laboured respiration or pilar erecti.…”

Section: Discussionmentioning

confidence: 99%

“…To this day, there is no standard for experimental gut inflammation models, and various research groups developed their own classification scores . Most models induce small intestine inflammation either through pathological agents (eg Heligmosomoides polygyrus ) or using transgenic mice (eg Roquin‐deficient mice), whereas our group targets to replicate the intestinal manifestations observed in humans allergic to food . The use of human classification scores prompted us to establish an equivalent classification system for experimental models of food allergy that is accompanied by small intestine inflammation.…”

Section: Introductionmentioning

confidence: 99%

“…16 Most models induce small intestine inflammation either through pathological agents 17 (eg Heligmosomoides polygyrus) or using transgenic mice 18 (eg Roquin-deficient mice), whereas our group targets to replicate the intestinal manifestations observed in humans allergic to food. 19 The use of human classification scores prompted us to establish an equivalent classification system for experimental models of food allergy that is accompanied by small intestine inflammation. Therefore, the aim of this work was to propose a classification system of intestinal histomorphology for experimental mice, covering quantitative and qualitative aspects, that allows the standardization of the states of normality and a grading of inflammation of the mucosal architecture of the mouse small intestine.…”

Section: Introductionmentioning

confidence: 99%

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A histomorphometric classification system for normal and inflamed mouse duodenum—Quali‐quantitative approach

Silva

Soares

Mattos

et al. 2018

Int J Experimental Path

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Gut-associated intestinal lymphoid tissue, the largest secondary lymphoid organ in the human body, constantly samples antigens from the gut lumen, presenting as a default response the activation of TCD4 FOXP3 regulatory T cells that secrete a profile of anti-inflammatory cytokines maintaining gut homeostasis denominated from an immunological perspective as mucosal tolerance. However, when antigens are sampled in an inflammatory setting, the immune response may either be protective, in the case of harmful pathogens, or cause further inflammatory reactions as in food allergy, inflammatory bowel diseases, coeliac disease or food protein-induced enterocolitis syndrome. Therefore, there is a need for accurate and consistent experimental models. However, a drawback in comparing these models is the lack of a classification system similar to that which is already used for humans. Thus, the aim of this work was to propose a classification system of the small intestinal histomorphology in experimental mice. To do this we used a mouse antigen-specific gut inflammation model developed by our research group. Duodenum sections stained with haematoxylin-eosin and Alcian blue were scanned using the APERIO scanning system and analysed with the ImageScope software. The evaluated parameters were villus area, villus height and width, enterocyte count, mononuclear intra-epithelial leucocyte and goblet cell counts, and architectural and cellular ratios. Food-sensitized animals challenged with a diet containing the corresponding food allergen presented, as for humans, time-dependent shortened and widened villi accompanied by leucocyte infiltrate and loss of goblet cells. With these data, we were able to establish a classification system for experimental intestinal inflammation in mice thus permitting better comparisons among and between experiments than has been possible previously.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A histomorphometric classification system for normal and inflamed mouse duodenum—Quali‐quantitative approach

Silva

Soares

Mattos

et al. 2018

Int J Experimental Path

Self Cite

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“…Previous studies from our group reported the inflammatory milieu of the gut of allergic animals submitted to oral challenge, as well as the normal millieu of the gut of allergic animals that were not submitted to oral challenge (23,24). The analysis performed in these papers used either OM or DM; however, no comparison was performed.…”

Section: Discussionmentioning

confidence: 99%

Comparison between digital and optical microscopy: Analysis in a mouse gut inflammation model

et al. 2017

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Abstract. Virtual microscopy is currently widely used for various purposes, such as teaching, archiving, collaborations and research. Although the cost of this technique has reduced, it continues to be expensive for the majority of laboratories in developing countries. The Graduate Program in Pathology at the Federal Fluminense University (Niterói, Brazil) has acquired equipment for virtual microscopy. However, this novel method faced prejudice, as students and technicians were skeptical about its reliability. Thus, the aim of the current study was to evaluate whether virtual microscopy is a reliable method of analysis for our research. Thus, a mouse gut inflammation model developed by our research group was used in the present study. Analysis was performed using optical microscopy and digital imaging using the APERIO scanning system and the ImageScope ® software. Intestinal epithelial cells (IECs), intra epithelial leucocytes (IEL), and villi number and area were evaluated. No significant differences were observed in villi number, IEC and IEL; however, the villi area was significantly smaller when measured using the computer. Thus, the present study indicates that virtual microscopy is a trustworthy method for research purposes.

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Maternal pomegranate juice attenuates maternal inflammation–induced fetal brain injury by inhibition of apoptosis, neuronal nitric oxide synthase, and NF-κB in a rat model

Ginsberg

Khatib

Saadi

et al. 2018

American Journal of Obstetrics and Gynecology

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Maternal immunomodulation of the offspring's immunological system

Cited by 10 publications

References 51 publications

A histomorphometric classification system for normal and inflamed mouse duodenum—Quali‐quantitative approach

A histomorphometric classification system for normal and inflamed mouse duodenum—Quali‐quantitative approach

Comparison between digital and optical microscopy: Analysis in a mouse gut inflammation model

Maternal pomegranate juice attenuates maternal inflammation–induced fetal brain injury by inhibition of apoptosis, neuronal nitric oxide synthase, and NF-κB in a rat model

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