Mast cells partly contribute to allergic enteritis development: Findings in two different mast cell‐deficient mice

Blanco-Pérez, Frank; González-Menéndez, Irene; Stassen, Michael; Kato, Yoichiro; Laiño, Jonathan Emiliano; Kirberg, Jörg; Krause, Maren; Martella, Manuela; Shibata, Noriyuki; Quintanilla-Martı́nez, Leticia; Feyerabend, Thorsten B.; Rodewald, Hans-Reimer; Galli, Stephen J.; Vieths, Stefan; Scheurer, Stephan; Toda, Masako

doi:10.1111/all.15182

Cited by 2 publications

(7 citation statements)

References 6 publications

(5 reference statements)

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“…After two rounds of sensitization, wild-type BALB/c mice were challenged with an egg white diet (EW diet) or OVA-free diet (OF diet) to induce food allergies. As previously reported, 14,15,19 this resulted in prototypical features of food allergies in the EW group, including decreased body weight (Fig. 1C) and body temperature (Fig.…”

Section: Resultssupporting

confidence: 83%

“…29 To test this, we systematically examine the levels of potential functioning granulocytes, including mast cells, eosinophils, and neutrophils in the mouse ileal mucosa. Consistent with previous reports, 14,30 we found that continuous food challenge raise the accumulation of mast cells in all three EW-fed groups, as assessed by the specific staining of IHC for the antitryptase (Fig. 4A and Fig.…”

Section: Luminal Eatp Promoted Mucosal Neutrophil Accumulationsupporting

confidence: 92%

“…The sensitized protocol was performed according to previous reports with several modifications. 14,15 In brief, after one-week stabilization, mice were intraperitoneally injected 50 μg of ovalbumin (A5503, Sigma-Aldrich) with 1 mg of aluminum potassium sulfate adjuvant (A7210, Sigma-Aldrich) once and then again 2 weeks later. Two weeks after the second sensitization, the mice were fed with an egg white diet (EW diet) containing ovalbumin (OVA) or OVA free diet (OF diet) that used soybean as the source of protein.…”

Section: Food Allergy Modelmentioning

confidence: 99%

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Extracellular adenosine triphosphate skews the T helper cell balance and enhances neutrophil activation in mice with food allergies

Zhang,

Chen,

Yang

et al. 2024

Food Funct.

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Section: Resultssupporting

confidence: 83%

Section: Luminal Eatp Promoted Mucosal Neutrophil Accumulationsupporting

confidence: 92%

Section: Food Allergy Modelmentioning

confidence: 99%

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Extracellular adenosine triphosphate skews the T helper cell balance and enhances neutrophil activation in mice with food allergies

Zhang,

Chen,

Yang

et al. 2024

Food Funct.

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“… 3 The GI mucosa is enriched with mast cells that are one of the primary effector cells of the allergic response. 4 , 5 , 6 They express the high affinity IgE receptor (FcɛRI) that binds allergen‐specific IgE antibodies. Upon exposure to a relevant allergen, the receptor‐bound IgE recognizes the allergen and crosslinks the receptors, activating the cell.…”

Section: Introductionmentioning

confidence: 99%

“…The gastrointestinal (GI) tract plays a central role in FA as the primary site of exposure and immune response to food allergens when oral or mucosal tolerance are not achieved 3 . The GI mucosa is enriched with mast cells that are one of the primary effector cells of the allergic response 4–6 . They express the high affinity IgE receptor (FcɛRI) that binds allergen‐specific IgE antibodies.…”

Section: Introductionmentioning

confidence: 99%

Precision cut intestinal slices, a novel model of acute food allergic reactions

Hung

Çelik

Yin

et al. 2022

Allergy

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Background Food allergy affects up to 10% of the pediatric population. Despite ongoing efforts, treatment options remain limited. Novel models of food allergy are needed to study response patterns downstream of IgE‐crosslinking and evaluate drugs modifying acute events. Here, we report a novel human ex vivo model that displays acute, allergen‐specific, IgE‐mediated smooth muscle contractions using precision cut intestinal slices (PCIS). Methods PCIS were generated using gut tissue samples from children who underwent clinically indicated surgery. Viability and metabolic activity were assessed from 0 to 24 h. Distribution of relevant cell subsets was confirmed using single nucleus RNA sequencing. PCIS were passively sensitized using plasma from peanut allergic donors or peanut‐sensitized non‐allergic donors, and exposed to various stimuli including serotonin, histamine, FcɛRI‐crosslinker, and food allergens. Smooth muscle contractions and mediator release functioned as readouts. A novel program designed to measure contractions was developed to quantify responses. The ability to demonstrate the impact of antihistamines and immunomodulation from peanut oral immunotherapy (OIT) was assessed. Results PCIS viability was maintained for 24 h. Cellular distribution confirmed the presence of key cell subsets including mast cells. The video analysis tool reliably quantified responses to different stimulatory conditions. Smooth muscle contractions were allergen‐specific and reflected the clinical phenotype of the plasma donor. Tryptase measurement confirmed IgE‐dependent mast cell‐derived mediator release. Antihistamines suppressed histamine‐induced contraction and plasma from successful peanut OIT suppressed peanut‐specific PCIS contraction. Conclusion PCIS represent a novel human tissue‐based model to study acute, IgE‐mediated food allergy and pharmaceutical impacts on allergic responses in the gut.

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Mast cells partly contribute to allergic enteritis development: Findings in two different mast cell‐deficient mice

Cited by 2 publications

References 6 publications

Extracellular adenosine triphosphate skews the T helper cell balance and enhances neutrophil activation in mice with food allergies

Extracellular adenosine triphosphate skews the T helper cell balance and enhances neutrophil activation in mice with food allergies

Precision cut intestinal slices, a novel model of acute food allergic reactions

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