1987
DOI: 10.1001/archderm.123.2.205
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Mast cell numbers in diffuse scleroderma

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Cited by 49 publications
(42 citation statements)
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“…Although the conclusions reported by those investigators are different from ours, the actual calculated mast cell concentrations in scleroderma skin lesions ranged between 6,000 and 14,300/mm3 in their study, not significantly different from the values reported herein; the difference in interpretation of the results stems from the use of different values for normal skin mast cell concentrations for comparison (mean 4 SD 4,762 * 1,333/mm3 in their study and 7,347 * 2,973/mm3 in ours). Similarly, Nishioka et a1 reported increased mast cell numbers in the papillary dermis of patients with early, but not late, scleroderma (10). Variations in section thickness, tissue fixation, method of staining, and method of counting may explain the differences among the various studies.…”
Section: Discussionmentioning
confidence: 95%
See 1 more Smart Citation
“…Although the conclusions reported by those investigators are different from ours, the actual calculated mast cell concentrations in scleroderma skin lesions ranged between 6,000 and 14,300/mm3 in their study, not significantly different from the values reported herein; the difference in interpretation of the results stems from the use of different values for normal skin mast cell concentrations for comparison (mean 4 SD 4,762 * 1,333/mm3 in their study and 7,347 * 2,973/mm3 in ours). Similarly, Nishioka et a1 reported increased mast cell numbers in the papillary dermis of patients with early, but not late, scleroderma (10). Variations in section thickness, tissue fixation, method of staining, and method of counting may explain the differences among the various studies.…”
Section: Discussionmentioning
confidence: 95%
“…The alkaline phosphatase reaction was developed with fast blue RR (Sigma) at 1 mg/ml in 0.1M Tris buffer, pH 8.2. containing 0.2 mg/ml of naphthol AS-MX phosphate (Sigma) for [10][11][12][13][14][15][16][17][18][19][20] minutes at room temperature in the dark. A blue color developed only in previously unstained MC, cells.…”
Section: Methodsmentioning
confidence: 99%
“…Twenty-two patients with RP and abnormal findings on capillaroscopy were included in this study. There were 1 man and 21 women, with a median age of 43 years (range 20-62) and a median disease duration of 3.5 years (range [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20]. The diagnosis of RP was based on the patients' medical history or on the observation of bilateral, episodic, cold-induced pallor and/or cyanosis of the fingers, or of both the fingers and toes.…”
Section: Methodsmentioning
confidence: 99%
“…They are mainly located in the skin, digestive tract, and lungs, which are the organs that are most frequently affected by SSc (18). In SSc lesions, mast cells are concentrated in sclerotic perivascular areas (19)(20)(21). In vitro experiments have demonstrated that heparin and histamine, which are both mast cell mediators, are able to stimulate proliferation of fibroblasts and collagen synthesis (22,23).…”
Section: Baseline Clinical Featuresmentioning
confidence: 99%
“…The tissue sections (n ϭ 10) were stained with H&E for overall morphological analysis, with May-Giemsa staining or toluidine blue method to detect degranulating mast cells, 18 or with Sirius Red F3BA (0.1% solution in saturated aqueous picric acid) to demonstrate collagen matrix indicating fibrous changes. 19 The progression of perivascular fibrosis was evaluated by comparing the perivascular area to the total vessel area, as described previously.…”
Section: Histopathological Analysismentioning
confidence: 99%